Establishment and analysis of novel animal models for neuromyelitis optica using aquaporin-4 knockout mice

• Project/Area Number: 22590940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Keio University
• Principal Investigator: ABE Yoichiro 慶應義塾大学, 医学部, 講師 (10317331)
• Co-Investigator(Kenkyū-buntansha): IKESHIMA Hiroko 慶應義塾大学, 医学部, 特任講師 (60265783)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 神経病態免疫学 / 脳神経疾患 / 免疫学 / 動物モデル / アストロサイト / アクアポリン / 視神経脊髄炎 / 多発性硬化症

Research Abstract

To establish mouse models for neuromyelitis optica, we developed two different kinds of high affinity monoclonal antibodies against the extracellular domains of mouse aquaporin-4 using the baculovirus display method. In addition, to express exogenous gene

Research Products

• [Journal Article] A novel monoclonal antibody against the C-terminal region of aquaporin-4. (2013)
  • Author(s): Julia Ramadhanti, Ping Huang, Osamu Kusano-Arai, Hiroko Iwanari, Toshiko Sakihama, Tasturo Misu, Kazuo Fujihara, Takao Hamakubo, Masato Yasui, and Yoichiro Abe
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4. (2013)
  • Author(s): Kaori Miyazaki, Yoichiro Abe, Hiroko Iwanari, Yota Suzuki, Takahiro Kikuchi, Takashi Ito, Jungo Kato, Osamu Kusano-Arai, Toshiyuki Takahashi, Shuhei Nishiyama, Hiroko Ikeshima-Kataoka
  • Journal Title: J. Neuroimmunol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin (2013)
  • Author(s): Hiroko Ikeshima-Kataoka, Yoichiro Abe, Takaya Abe, and Masato Yasui.
  • Journal Title: Mol. Cell. Neurosci. Volume: 56 Pages: 65-75
  • Peer Reviewed
• [Journal Article] Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin (2013)
  • Author(s): Ikeshima-Kataoka H.
  • Journal Title: Mol. Cell. Neurosci. Volume: 未定 Pages: 65-75
  • DOI: 10.1016/j.mcn.2013.02.002
  • Peer Reviewed
• [Journal Article] Establishment of Monoclonal Antibodies Against the Extracellular Domain That Block Binding of NMO-IgG to AQP4 (2013)
  • Author(s): Miyazaki K.
  • Journal Title: J. Neuroimmunol. Volume: 未定 Issue: 1-2 Pages: 107-16
  • DOI: 10.1016/j.jneuroim.2013.03.003
  • Peer Reviewed
• [Journal Article] antibody against the C-terminal region of aquaporin-4 (2013)
  • Author(s): Ramadhanti J.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 未定
  • Peer Reviewed
• [Journal Article] An astrocyte-specific enhancer of aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements. (2012)
  • Author(s): Yoichiro Abe, Hiroko Ikeshima-Kataoka
  • Journal Title: J. Neurochem. Volume: 120 Pages: 899-912
  • Peer Reviewed
• [Journal Article] An astrocyte-specific enhancer of aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements (2012)
  • Author(s): Abe Y., Ikeshima-Kataoka H., et al
  • Journal Title: J.Neurochem. Volume: 120 Pages: 899-912
  • Peer Reviewed
• [Journal Article] Curcumin and genistein additively potentiate G551D-CFTR (2011)
  • Author(s): Yu Y-C, Miki H, Nakamura Y, Hanyuda A, Matsuzaki Y, Abe Y, et al
  • Journal Title: J.Cyst.Fibros Volume: 10 Pages: 243-253
  • Peer Reviewed
• [Presentation] Development of monoclonal antibodies against the extracellular domains of mouse AQP4 and its application to elucidating the disease mechanism of neuromyelitis optica. (2013)
  • Author(s): A b e , Y., Iwanari, H., Arai, O., Ramadhanti, J., Huang, P., Miyauchi, T., Kurihara, N., Miyazaki, K., Ikeshima-Kataoka, H., Sakihama, T., Hamakubo, T, and Yasui, M.
  • Organizer: The 86th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Fukuoka
• [Presentation] Development of monoclonal antibodies against the extracellular domains of mouse AQP4 and its application to elucidating the disease mechanism of neuromyelitis optica (2013)
  • Author(s): 阿部陽一郎
  • Organizer: 第８６回日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] アクアポリン4 M23プロモーターのアストロサイトに置ける転写調節 (2012)
  • Author(s): 阿部陽一郎、池島宏子
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2012-03-16
• [Presentation] マウス損傷脳のグリア細胞活性化におけるアクアポリン4の機能解析 (2012)
  • Author(s): 池島宏子、阿部陽一郎
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Regulation and in vivo function of square array formation of aquaporin-4. (2012)
  • Author(s): A b e , Y., Goda, W., Ramadhanti, J., Ikeshima-Kataoka, H., and Yasui, M.
  • Organizer: The 35th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
• [Presentation] Transcriptional regulation of the mouse aquaporin-4 M23 promoter in astrocytes. (2012)
  • Author(s): Abe, Y., Ikeshima-Kataoka, H., Goda, W., and Yasui, M.
  • Organizer: The 85th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Kyoto
• [Presentation] Development of monoclonal antibodies that block binding of NMO-IgG to AQP4. (2012)
  • Author(s): Miyazaki, K., Abe, Y., Hamakubo, T., Fujihara, K., and Yasui, M.
  • Organizer: The 85th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Kyoto
• [Presentation] Regulation and in vivo function of square array formation of aquaporin-4. (2012)
  • Author(s): 阿部陽一郎
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] A consensus sequence of POU transcription factors ins indispensable for transcriptional regulation of mouse aquaporin-4 gene in astrocytes (2011)
  • Author(s): 阿部陽一郎、池島宏子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] アストロサイトにおけるマウスアクアポリン4遺伝子の転写に関与するシスーエレメントの同定 (2011)
  • Author(s): 阿部陽一郎、池島宏子
  • Organizer: 第84回薬理学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-03-22
• [Presentation] A consensus sequence of POU transcription factors is indispensable for transcriptional regulation of mouse aquaporin-4 gene in astrocytes. (2011)
  • Author(s): Abe, Y., Ikeshima-Kataoka, H., Goda, W., and Yasui, M.
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
• [Presentation] Identification of cis-element involved in transcriptional regulation of mouse aquaporin-4 gene in astrocytes. (2011)
  • Author(s): A b e , Y., Ikeshima-Kataoka, H., and Yasui, M.
  • Organizer: The 84th Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
• [Remarks]
  • URL: http://www.pharm.med.keio.ac.jp/index.html
• [Remarks] 慶應義塾大学医学部薬理学教室
  • URL: http://www.pharm.med.keio.ac.jp/index.html