| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The purpose of our study was to clarify whether S-nitrosylation induced excessive mitochondrial fission and fragmentation in the brain. We stereotactically administered glyceryl trinitrate in the mouse brain of both triple transgenic (3xTg) and wild type (Wt) mice, and measured SNO-Drp1 by biotin switch assay and compared mitochondrial morphology by deconvolution fluorescence microscopy using mito-DsRed2 staining. SNO-Drp1 formation was increased in a dose-dependent manner induced by glyceryl trinitrate, and this phenomenon was more prominent in 3xTg than in Wt mice. Mito-DsRed2 staining showed excessive mitochondrial fission and fragmentation in the brain of 3xTg but not prominently in the brain of Wt mice. These results suggest that β-amyloid may cause excessive mitochondrial fragmentation and mitochondrial dysfunction by induction of excessive SNO-Drp1 production.
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