Roles of KATP channel/insulin signaling in the hypothalamus on the regulation of glucose homeostasis

• Project/Area Number: 22590970
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Chiba University
• Principal Investigator: MIKI Takashi 千葉大学, 大学院・医学研究院, 教授 (50302568)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エネルギー / 糖質代謝異常 / 視床下部 / KATPチャネル / インスリン / K_<ATP>チャネル / 脂肪組織

Research Abstract

KATP channel is suggested to regulate cellular excitability of various cell types by acting as a metabolic sensor. Especially, the channel in the hypothalamus, the metabolic center in the body, plays an important role in metabolic regulation of insulin targeted tissues. In the present study, we found that insulin signaling in the adipose tissues is modulated exogenously by the brain KATP channel and that KATP channel is involved in the glucose sensing in several nuclei in the brain stem as well as the hypothalamus.

Research Products

• [Journal Article] A case of Type 1 diabetes with nocturnal hypoglycemia after desensitization therapy for insulin allergy (2013)
  • Author(s): Kitamoto, T., Sakurai, K., Tachibana K., Yokoh H., Ishikawa, K., Miki, T., Yokote K
  • Journal Title: Diabetes Care Volume: (in press)
  • Peer Reviewed
• [Journal Article] Refeeding with a standard diet after a 48-h fast elicits an inflammatory response in the mouse liver (2013)
  • Author(s): Oarada M, Miki T, Kohno S, Sakai K, Nikawa T, Yoneyama M, Gonoi T.
  • Journal Title: J Nutr Biochem Volume: (in press) Issue: 7 Pages: 1314-1323
  • DOI: 10.1016/j.jnutbio.2012.10.006
  • Peer Reviewed
• [Journal Article] A case of Type 1 diabetes with nocturnal hypoglycemia after desensitization therapy for insulin allergy. (2013)
  • Author(s): Kitamoto, T
  • Journal Title: Diabetes Care Volume: in press
• [Journal Article] A novel function of Noc2 in agonist-induced intracellular Ca2+ increase during zymogen-granule exocytosis in pancreatic acinar cells. (2012)
  • Author(s): Ogata, S., Miki, T., Seino, S., Tamai, S., Kasai, H. & Nemoto, T.
  • Journal Title: PLoS ONE Volume: e37048 Issue: 5 Pages: 1-9
  • DOI: 10.1371/journal.pone.0037048
  • Peer Reviewed
• [Journal Article] Glucagon-like peptide-1 secretion by direct stimulation of L cells with luminal sugar versus non-nutritive sweetener (2012)
  • Author(s): Sakurai, K., Lee, EY., Morita, A., Kimura, S., Kawamura, H., Kasamatsu, A.,Shiiba, M., Yabe, D., Yokote,K., Miki, T
  • Journal Title: J Diabetes Invest Volume: 3 Issue: 2 Pages: 156-63
  • DOI: 10.1111/j.2040-1124.2011.00163.x
  • Peer Reviewed
• [Journal Article] Beneficial effects of ventromedial hypothalamus (VMH) lesioning on functionand morphology of the liver after hepatectomy in rats (2011)
  • Author(s): Lee, EY., Inoue, S., Senoo, A., Shimizu, H., Suzuki Y., Ishizuka, N., Imazeki, N., Sasaki, K., Kako, M., Osaka, T., Miki, T.
  • Journal Title: Brain Res Volume: 1421 Pages: 82-9
  • DOI: 10.1016/j.brainres.2011.09.012
  • Peer Reviewed
• [Journal Article] Pancreatic β-cells are generated by neogenesis from non-β-cells after birth (2011)
  • Author(s): Nakamura, K., Minami, K., Tamura, K., Iemoto, K., Miki,T., Seino, S.
  • Journal Title: Biomed Res Volume: 3 Pages: 167-74
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21551953
  • Peer Reviewed
• [Journal Article] Inhibition of ATP-Sensitive K(+) Channels and L-Type Ca(2+) Channels by Amiodarone Elicits Contradictory Effect on Insulin Secretion in MIN6 Cells (2011)
  • Author(s): Nishida, A., Takizawa, T., Matsumoto, A., Miki,T., Seino, S., Nakaya, H.
  • Journal Title: J Pharmacol Sci Volume: 116(1) Pages: 73-80
  • NAID: 10029894018
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21512308
  • Peer Reviewed
• [Journal Article] Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice (2011)
  • Author(s): Iwanaga, T., Miki,T., Takahashi- Iwanaga, H.
  • Journal Title: Biomedical Research Volume: 32 Issue: 1 Pages: 73-81
  • DOI: 10.2220/biomedres.32.73
  • NAID: 130004903819
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Inhibition of ATP-sensitive K^+ channels and L-type Ca^<2+> channels by a miodarone elicits contradictory effect on insulin secretion in MIN6 cells (2011)
  • Author(s): Nishida A, et al.
  • Journal Title: J Pharmacol Sci Volume: 116 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Pancreatic β-cells are generated by neogenesis from non-β-cells after birth (2011)
  • Author(s): Nakamura K, et al.
  • Journal Title: Biomed Res Volume: 3 Pages: 167-174
  • Peer Reviewed
• [Journal Article] Beneficial effects of ventromedial hypothalamus (VMH) lesioning on function and morphology of the liver after hepatectomy in rats (2011)
  • Author(s): Lee EY, et al.
  • Journal Title: Brain Res Volume: 1421 Pages: 82-89
  • Peer Reviewed
• [Journal Article] Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenophysis of mice. (2011)
  • Author(s): Iwanaga, T., et al.
  • Journal Title: Biomedical Research Volume: 32 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Establishment of new clonal pancreatic β-cell lines (MIN6-K) useful for study of incretin/cAMP signaling (2010)
  • Author(s): Iwasaki, M., Minami K., Shibasaki, T., Miki,T., Jun-ichi Miyazaki, J-I., Seino, S.
  • Journal Title: J Diabetes Invest Volume: 4 Issue: 4 Pages: 137-142
  • DOI: 10.1111/j.2040-1124.2010.00026.x
  • Peer Reviewed
• [Journal Article] Rim2a determines docking and priming states in insulin granule exocytosis (2010)
  • Author(s): Yasuda T, Shibasaki T, Minami K, Takahashi H, Mizoguchi A, Uriu Y, Numata T, Mori Y, Miyazaki JI, Miki T & Seino S
  • Journal Title: Cell Metab. Volume: 12 Issue: 2 Pages: 117-129
  • DOI: 10.1016/j.cmet.2010.05.017
  • Peer Reviewed
• [Journal Article] Rim2 α determines docking and priming states in insulin granule exocytosis. (2010)
  • Author(s): Yasuda, T., et al.
  • Journal Title: Cell Metab Volume: 12(2) Pages: 117-129
  • Peer Reviewed
• [Journal Article] Establishment of new clonal pancreatic fl -cell lines(MIN6-K)useful for study of incretin/ cAMP signaling. (2010)
  • Author(s): Iwasaki, M., et al.
  • Journal Title: J Diabetes Invest Volume: 4 Pages: 137-142
  • Peer Reviewed
• [Presentation] 腸管内分泌L細胞 からの糖によるGLP-1 (2013)
  • Author(s): Takashi Miki
  • Organizer: 分泌メカニズム第90回日本生理学会
  • Place of Presentation: 東京
• [Presentation] SDTラットでは膵島内のVEGFシグナル亢進がβ細胞を障害し糖尿病を引き起こす (2013)
  • Author(s): 向英里
  • Organizer: 第27回 日本糖尿病・肥満動物学会年次学術集会
  • Place of Presentation: 東京
• [Presentation] 腸管内分泌Ｌ細胞からの糖によるＧＬＰ-1分泌メカニズム (2013)
  • Author(s): Takashi Miki
  • Organizer: 第90回日本生理学会
  • Place of Presentation: 東京
• [Presentation] Enhanced VEGF signaling in islets contributes to β-cell injury and consequential diabetes in SDT rats (2012)
  • Author(s): Mukai, E., Ohta, T., Kawamura, H., Lee, E.Y., Morita, A., Inagaki, N., Iwanaga, T., Miki, T.
  • Organizer: 9th IDF-WPR Congress & 4th AASD Scientific Meeting
  • Place of Presentation: Kyoto, Japan
• [Presentation] Increased gluconeogenesis from glycerol plays a critical role in developing diabetes under high fat diet (2012)
  • Author(s): Lee, EY., Sakurai, K., Toda,C., Yokote, K., Minokoshi, Y, Miki, T.
  • Organizer: the 9th IDF-WPR Congress & 4th AASD Scientific Meeting
  • Place of Presentation: Kyoto, Japan
• [Presentation] Increased gluconeogenesis from glycerol plays a critical role in developing diabetes under high fat diet. (2012)
  • Author(s): Lee, EY.
  • Organizer: 9th IDF-WPR Congress & 4th AASD Scientific Meeting
  • Place of Presentation: Kyoto
• [Presentation] Enhanced VEGF signaling in islets contributes to β-cell injury and consequential diabetes in SDT rats. (2012)
  • Author(s): Mukai, E.
  • Organizer: 9th IDF-WPR Congress & 4th AASD Scientific Meeting
  • Place of Presentation: Kyoto
• [Presentation] Effect of an alpha-glucosidase inhibitor acarbose on glucagon-like peptide-1 secretion and postprandial lipid profile (2011)
  • Author(s): Sakurai, K, et al.
  • Organizer: AASD2011 3rd Annual Meeting
  • Place of Presentation: Bijing, China
  • Year and Date: 2011-07-22
• [Presentation] 誘導性Cre/loxPシステムを用いた膵β細胞の運命追跡 (2011)
  • Author(s): 田村香楠子, 他
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-20
• [Presentation] 健常日本人におけるGLP-1分泌動態の検討 (2011)
  • Author(s): 櫻井健一, 他
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-19
• [Presentation] Glucagon-like peptide 1 (GLP-1)分泌におけるNoc2の役割 (2011)
  • Author(s): 岸本亜野, 他
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-19
• [Presentation] Effect of an alpha-glucosidase inhibitor acarbose on glucagon-like peptide-1 secretion and postprandial lipid profile (2011)
  • Author(s): Sakurai, K., Lee, EY, Morita, A., Yabe, D., Yokote, K., Miki, T.
  • Organizer: AASD2011 3rd Annual Meeting
  • Place of Presentation: Beijing, China
• [Presentation] Cellular mechanism for GLP-1 specific insulinotropic action in pancreatic β-cells (2010)
  • Author(s): Takashi Miki, Susumu Seino
  • Organizer: 第53回日本糖尿病学会年次学術集 会.(糖尿病 53, S37, 2010)
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-28
• [Presentation] Cellular mechanism for GLP-1 specic insulinotropic action in pancreatic β-cell. (2010)
  • Author(s): Takashi Miki, Susumu Seino
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-28
• [Presentation] 膵β細胞内グルコース代謝とcAMPシグナルとの相互作用 (2010)
  • Author(s): 岩崎真宏, 南幸太郎, 柴崎忠雄, 中山泰宗, 原田和生, 馬場健史, 三木隆司, 宮崎純一, 福崎英一郎, 清野進
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-27
• [Presentation] インスリン顆粒の開口放出機構におけるRim2αの役割 (2010)
  • Author(s): 安田貴雄, 柴崎忠雄, 三木隆司, 高橋晴美, 宮崎純一, 南幸太郎, 清野進
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-27
• [Book] GLP-1分泌に関する最新の知 (2013)
  • Author(s): 三木隆司
  • Publisher: Progress in Medicine 33
• [Book] インクレチン関連薬の現状と課題 (2013)
  • Author(s): 三木隆司
  • Total Pages: 222
  • Publisher: ライフサイエンス社
• [Book] 各栄養素による膵β細胞におけるインスリン分泌と代謝制御 (2012)
  • Author(s): 三木隆司
  • Publisher: 内分泌糖尿病代謝内科
• [Book] 栄養素の受容・情報伝達と代謝制御 (2012)
  • Author(s): 三木隆司
  • Total Pages: 384
  • Publisher: 科学評論社
• [Book] 京都廣川書店 (2011)
  • Author(s): 三木隆司
  • Publisher: 細胞内外のATPセンサーであるATP感受性K+チャネルとATP受容体.トランスポートソームの世界-膜輸送研究の源流から未来へ-
• [Book] 肝臓の輸送機能と代謝機能.ギャノング生理学原書23版,岡田泰伸監訳 (2011)
  • Author(s): 三木隆司
  • Publisher: 丸善
• [Book] 転写因子Dmbx1欠損マウスのやせの病態解析 (2011)
  • Author(s): 李恩瑛,三木隆司
  • Publisher: Adipo science 7
• [Book] ギャノング生理学 肝臓の輸送機能と代謝機能原書23版(岡田泰伸監訳) (2011)
  • Author(s): 三木隆司
  • Total Pages: 838
  • Publisher: 丸善
• [Book] トランスポートソームの世界-膜輸送研究の源流から未来へ-細胞内外のATPセンサーであるATP感受性K^+チャネルとATP受容体 (2011)
  • Author(s): 三木隆司
  • Total Pages: 471
  • Publisher: 京都廣川書店
• [Book] Adipo science転写因子Dmbx1欠損マウスのやせの病態解析 (2011)
  • Author(s): 李恩瑛、三木隆司
  • Publisher: フジメディカル出版
• [Book] ギャノング生理学原書23版 (2011)
  • Author(s): 三木隆司
  • Total Pages: 838
  • Publisher: 丸善株式会社
• [Book] GLP-1の血糖降下作用のメカニズムは? (2010)
  • Author(s): 三木隆司
  • Publisher: 肥満と糖尿病9
• [Book] 肥満と糖尿病9 (2010)
  • Author(s): 三木隆司
  • Total Pages: 144
  • Publisher: 株式会社丹水社
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/physiol/index.html
• [Remarks] 千葉大学大学院医学研究院代謝生理学ホームページ
  • URL: http://www.m.chiba-u.ac.jp/class/physiol/
• [Remarks] 研究室ホームページ
  • URL: http://www.m.chiba-u.ac.jp/class/physiol/
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/physiol/