| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
To visualize intracellular ATP levels in physiological conditions, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP. Using the fluorescent ATP probe and the fluorescent Ca^<2+>probe Fura2-AM, we carried out dual measurements of intracellular ATP and Ca^<2+>concentrations of living pancreatic beta-cell lines in the physiological conditions. And we also generated the transgenic (Tg) mouse expressing ATP probe protein in pancreatic beta-cells. We are now carrying out several functional experiments using the Tg mouse.
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