Real-time measurement of intracellular ATP concentration using novel fluorescent ATP probe.
| Project/Area Number |
22590977
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Hiromi 京都大学, 生命科学研究科, 特定准教授 (20422545)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エネルギー / 糖質代謝異常 / ATP / K_ATP チャネル / 糖尿病 / KATPチャネル / 糖代謝 / K_<ATP>チャネル |
|---|
| Research Abstract |
To visualize intracellular ATP levels in physiological conditions, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP. Using the fluorescent ATP probe and the fluorescent Ca^<2+>probe Fura2-AM, we carried out dual measurements of intracellular ATP and Ca^<2+>concentrations of living pancreatic beta-cell lines in the physiological conditions. And we also generated the transgenic (Tg) mouse expressing ATP probe protein in pancreatic beta-cells. We are now carrying out several functional experiments using the Tg mouse.
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Report
(4 results)
Research Products
(93 results)
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[Journal Article] Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction2013
Author(s)
Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K, Inagaki N
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Journal Title
Diabetes
Volume: 62
Issue: 6
Pages: 1996-2003
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcriptional regulatory factor X6 (Rfx6) increases gastric inhibitory polypeptide (GIP) expression in enteroendocrine K-cells and is involved in GIP hypersecretion in high fat diet-induced obesity.2013
Author(s)
Suzuki K, Harada N, Yamane S, Nakamura Y, Sasaki K, Nasteska D, Joo E, Shibue K, Harada T, Hamasaki A, Toyoda K, Nagashima K, Inagaki N.
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Journal Title
J Biol Chem.
Volume: 288
Issue: 3
Pages: 1929-1938
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] GCKR mutations in Japanese families with clustered type 2 diabetes2011
Author(s)
Tanaka D, Nagashima K, Sasaki M, Yamada C, Funakoshi S, Akitomo K, Takenaka K, Harada K, Koizumi A, M, Inagaki N
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Journal Title
Mol. Genet. Metab
Volume: 102
Issue: 4
Pages: 453-460
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Molecular analyses of Japanese patients with neonatal diabetes2012
Author(s)
Nagashima K, Yorifuji T, Sasaki M, Tanaka D, Ogura K, Sato H, Tahara Y, Yamano G, Sato Y, Sugizaki K, Ogura M, Inagaki N
Organizer
9th International Diabetes Federation Western Pacific Region Congress/ 4th Scientific Meeting of the Asian Association for the Study of Diabetes
Place of Presentation
Kyoto
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