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Toward understanding the glucose- -cells from analysis of glucose-responsive MafA kinase

Research Project

Project/Area Number 22590982
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionNara Institute of Science and Technology

Principal Investigator

KATAOKA Kohsuke  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (20262074)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエネルギー・糖質代謝異常・細胞内シグナル伝達・遺伝子発現制御 / タンパク質リン酸化 / 遺伝子発現調節 / 糖尿病 / 細胞内局在 / タンパク質 / リン酸化 / 遺伝子発現制御 / インスリン / 転写調節因子複合体
Research Abstract

We have found that multiple phosphorylation events on MafA transcription factor by GSK3 and MaCK are required for its function in β-cells through enhancing DNA-binding and Beta2 association. Furthermore, We demonstrated that decreased MafA phosphorylation is one of the main causes of β-cell dysfunction in type II diabetes. We also identified a small molecule inhibitor that mimics endogenous MafA de-phosphorylation in β-cells. These findings led to understanding of molecular mechanisms of β-cell function and dysfunction.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (4 results)

  • [Journal Article] ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription2011

    • Author(s)
      Song-iee Han, Kunio Yasuda, Kohsuke Kataoka
    • Journal Title

      J Biol Chem

      Volume: 286(12) Issue: 12 Pages: 10449-56

    • DOI

      10.1074/jbc.m110.209510

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteasome activator PA28γ stimulates degradation of GSK3-phosphorylated insulin transcription activator MAFA2011

    • Author(s)
      Kanai, K., S. Aramata, S. Katakami, K. Yasuda, and K. Kataoka
    • Journal Title

      J. Mol. Endocrinol

      Volume: 47 Issue: 1 Pages: 119-127

    • DOI

      10.1530/jme-11-0044

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MafB interacts with Gcm2 and regulates parathyroid hormone expression and parathyroid development2011

    • Author(s)
      Kamitani-Kawamoto A, Hamada M, Moriguchi T, Miyai M, Hitoshi S, Ikenaka K, Hosoya T, Hotta Y, Takahashi S, Kataoka K
    • Journal Title

      J Bone Mineral Res

      Volume: 26 Issue: 10 Pages: 2463-2472

    • DOI

      10.1002/jbmr.458

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and Beta2, and activates insulin gene transcription.2011

    • Author(s)
      S.-i.Han
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 10449-10456

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insulin transactivator MafA regulates intra-thymic expression of insulin and affects susceptibility to type I diabetes2010

    • Author(s)
      Noso, S., K. Kataoka, Y. Kawabata, N. Babaya, Y. Hiromine, K. Yamaji, Y. Fujisawa, S. Aramata, T. Kudo, S. Takahashi, and H. Ikegami
    • Journal Title

      Diabetes

      Volume: 59 Pages: 2579-2587

    • Related Report
      2012 Final Research Report
  • [Journal Article] SUMOylation negatively regulates transcriptional and oncogenic activities of MafA2010

    • Author(s)
      Kanai K., H.M. Reza, A. Kamitani, Y. Hamazaki, S.-i. Han, K. Yasuda, and K. Kataoka
    • Journal Title

      Genes Cells

      Volume: 15 Pages: 971-982

    • Related Report
      2012 Final Research Report
  • [Journal Article] SUMOylation negatively regulates transcriptional and oncogenic activities of MafA.2010

    • Author(s)
      K.Kanai
    • Journal Title

      Genes to Cells.

      Volume: 15 Pages: 971-982

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insulin transactivator MafA regulates intra-thymic expression of insulin and affects susceptibility to type I diabetes.2010

    • Author(s)
      S.Noso
    • Journal Title

      Diabetes

      Volume: 59 Pages: 2579-2587

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Phosphorylations regulate transcriptional and non-transcriptional activity of MafA in oncogenic transformation2010

    • Author(s)
      K. Kanai
    • Organizer
      9thEMBL Conference Transcription and Chromatin
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2010-08-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Phosphorylations regulate transcriptional and non-transcriptional activity of MafA in oncogenic transformation.2010

    • Author(s)
      K.Kanai
    • Organizer
      9^<th> EMBL Conference Transcription and Chromatin
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2010-08-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] リン酸化による MafA の転写活性化能および新規の活性の制御機構の解明2010

    • Author(s)
      金井賢一
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸化によるMafAの転写活性化能および新規の活性の制御機構の解明2010

    • Author(s)
      金井賢一
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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