Functional analysis of the novel intestinal hormone, IBCAP withincretin-like activity

• Project/Area Number: 22590992
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Saitama Medical University
• Principal Investigator: TOYOSHIMA Hideo 埼玉医科大学, 医学部, 客員准教授 (20197966)
• Co-Investigator(Renkei-kenkyūsha): OKAZAKI Yasushi 埼玉医科大学, 医学部, 教授 (80280733) ; YOKOO Tomotaka 埼玉医科大学, 医学部, 助教 (80400688)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖尿病 / 消化管ホルモン / 新規分泌タンパク

Research Abstract

We identified a gene of IBCAP with an incretin-like activity. We developed to assessment of IBCAP activity as an indexing of beta-cells proliferation, and we identified candidate of sequences activated peptides of IBCAP gene. Also, transgenic mice over-expressing IBCAP was demonstrated to have increased amount of pancreatic β-cells, whereas gene-knockouts of the factor resulted in a marked decrease of pancreatic islets. Our findings strongly suggested IBCAP as a major player in the control of the amount of pancreatic islets and β-cells. Our results demonstrated that we assume that IBCAP can be another potential therapeutic target for diabetes.

Research Products

• [Journal Article] Expression of IBCAP (intestine-derived beta-cell augmenting promoter), an intestine specific secretory factor, induces insulin positive cells in the liver (2012)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Journal Title: Diabetologia Volume: 55(Suppl1) Issue: S1 Pages: 1-538
  • DOI: 10.1007/s00125-012-2688-9
  • Peer Reviewed
• [Journal Article] Central diabetes insipidus and hypothalamic type of hypopituitarism associated with atypical location of Rathke's cleft cyst (2012)
  • Author(s): Asano T, Aoki A, Sasaki M, Ikoma A, Toyoshima H, Kawakami M, Fujisawa I, Ishikawa SE
  • Journal Title: Intern Med Volume: 51 Pages: 189-94
  • NAID: 130002062056
  • Peer Reviewed
• [Journal Article] Expression of IBCAP (intestine-derived beta-cell augmenting promoter), an intestine specific secretory factor, induces insulin positive cells in the liver. (2012)
  • Author(s): Tomotaka YOKOO, Kazuhisa WATANABE, Kaoruko Tada IIDA, Hiroaki SUZUKI, Hitoshi SHIMANO, Masanobu KAWAKAMI, Nobuhiro YAMADA, Yasushi OKAZAKI, Hideo TOYOSHIMA
  • Journal Title: Diabetologia Volume: 55 Suppl 1
  • Peer Reviewed
• [Journal Article] IBCAP, Intestine- specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Yokoo T, Suzuki H, Shimano H, Watanabe K, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Journal Title: Diabetes Volume: 60(Suppl1) Issue: Supplement_1 Pages: A521-A548
  • DOI: 10.2337/db11-1929-2041
  • Peer Reviewed
• [Journal Article] Ectopic ACTH Syndrome Associated with Large Cell Neuroendocrine Carcinoma of the Thymus (2011)
  • Author(s): Saito T, Kimoto M, Nakai S, Ikoma A, Toyoshima H, Kawakami M, Nokubi M, Ishikawa SE
  • Journal Title: Internal Medicine Volume: 50 Issue: 14 Pages: 1471-1475
  • DOI: 10.2169/internalmedicine.50.5160
  • NAID: 130000969424
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] IBCAP, Intestine-specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Yokoo T, Suzuki H, Shimano H, Watanabe K, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Journal Title: Diabetes Volume: 60 Suppl 1
  • Peer Reviewed
• [Journal Article] Bilateral Aldosteronoma Associated with Secondary Aldosteronism in a Chronic Hemodialysis Subject (2010)
  • Author(s): Ikoma A, Saito T, Murata M, Toyoshima H, Nakamura Y, Kawakami M, Sasano H, Ishikawa SE
  • Journal Title: Internal Medicine Volume: 49 Issue: 11 Pages: 1017-1021
  • DOI: 10.2169/internalmedicine.49.2808
  • NAID: 130000251581
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Transgenic mice expressing an intestine-specific secretory protein, IBCAP, demonstrates pancreatic beta cell augmenting activity (2010)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Ishikawa SE, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Journal Title: Diabetologia Volume: 53(Suppl1)
  • Peer Reviewed
• [Journal Article] Transgenic Mice Expressing an Intestine-Specific Secretory Protein, IBCAP (Formerly CF266), Demonstrates Pancreatic β-Cell Augmenting Activity (2010)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Ishikawa SE, Kawakami M, Yamada N, Toyoshima H
  • Journal Title: Endocrine Reviews Volume: 31(3) Suppl 1
  • URL: http://www.endojournals.org/site/abstracts/P1-1_to_P1-729.pdf
  • Peer Reviewed
• [Journal Article] Transgenic Mice Expressing an Intestine-Specific Secretory Protein, IBCAP (Formerly CF266), Demonstrates Pancreatic ss-Cell Augmenting Activity. (2010)
  • Author(s): Tomotaka YOKOO
  • Journal Title: Endocrine Reviews Volume: 31(3)Suppl 1
  • Peer Reviewed
• [Journal Article] Transgenic mice expressing an intestine-specific secretory protein, IBCAP, demonstrates pancreatic beta cell augmenting activity (2010)
  • Author(s): Tomotaka YOKOO
  • Journal Title: Diabetologia Volume: 53(Suppl1)
  • Peer Reviewed
• [Presentation] Expression of IBCAP (intestine-derived beta-cell augmenting promoter), an intestine specific secretory factor, induces insulin positive cells in the liver (2012)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Organizer: 48th EASD Annual Meeting
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2012-10-02
• [Presentation] Expression of IBCAP (intestine-derived beta-cell augmenting promoter), an intestine specific secretory factor, induces insulin positive cells in the liver. (2012)
  • Author(s): Tomotaka YOKOO, Kazuhisa WATANABE, Kaoruko Tada IIDA, Hiroaki SUZUKI, Hitoshi SHIMANO, Masanobu KAWAKAMI, Nobuhiro YAMADA, Yasushi OKAZAKI, Hideo TOYOSHIMA
  • Organizer: 第48回 欧州糖尿病学会議 (EASD)
  • Place of Presentation: Messe Berlin（ドイツ、ベルリン）
• [Presentation] Effect of IBCAP, a Novel Intestine-specific Secretory Protein, on Pancreatic β-cell Differentiation. (2012)
  • Author(s): Tomotaka YOKOO, Kazuhisa WATANABE, Kaoruko Tada IIDA, Hiroaki SUZUKI, Hitoshi SHIMANO, Nobuhiro YAMADA, Yasushi OKAZAKI, Hideo TOYOSHIMA
  • Organizer: 第10回 RCGMフロンティア国際シンポジウム
  • Place of Presentation: 埼玉医科大学（埼玉県日高市）
• [Presentation] Demonstration of IBCAP as a Novel Intestine- specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Organizer: 第34回 日本分子生物学学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-14
• [Presentation] Demonstration of IBCAP as a Novel Inte stine-specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Tomotaka Yokoo
  • Organizer: 第34回日本分子生物学学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-12-14
• [Presentation] IBCAP; discovery of another intestinal hormone with beta cell augmenting activity (2011)
  • Author(s): Toyoshima H [invited speaker]
  • Organizer: The 2011 International Conference on Diabetes and Metabolism
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-11-11
• [Presentation] IBCAP ; discovery of another intestinal hormone with beta cell augmenting activity (2011)
  • Author(s): Hideo Toyoshima
  • Organizer: The 2011 International Conference on Diabetes and Metabolism
  • Place of Presentation: Seoul (Korea)(招待講演)
  • Year and Date: 2011-11-11
• [Presentation] 膵β細胞増加促進作用を持つ新規消化管ホルモンIBCAPの解析 (2011)
  • Author(s): 横尾友隆
  • Organizer: 第9回RCGMフロンティアシンポジウム
  • Place of Presentation: 埼玉県日高市
  • Year and Date: 2011-11-03
• [Presentation] 新規消化管ホルモン因子IBCAPによる膵β細胞増加作用の解析 (2011)
  • Author(s): 横尾友隆、渡邊和寿、飯田薫子、鈴木浩明、島野仁、石川三衛、川上正舒、山田信博、岡崎康司、豊島秀男
  • Organizer: 第26回 日本糖尿病合併症学会
  • Place of Presentation: さいたま市
  • Year and Date: 2011-10-14
• [Presentation] 新規消化管ホルモン因子IBCAPによる膵β細胞増加作用の解析 (2011)
  • Author(s): 横尾友隆
  • Organizer: 第26回日本糖尿病合併症学会
  • Place of Presentation: 埼玉県さいたま市
  • Year and Date: 2011-10-14
• [Presentation] IBCAP, Intestine-specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Ishikawa SE, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Organizer: The 71st Scientific Sessions of America Diabetes Association
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-06-27
• [Presentation] IBCAP, Intestine-specific Secretory Protein with Incretin-like Activity (2011)
  • Author(s): Tomotaka Yokoo
  • Organizer: The 71^<st> Scientific Sessions of America Diabetes Association
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2011-06-27
• [Presentation] 膵 β 細胞増加作用を持つ新規消化管特異的分泌タンパクIBCAPの機能解析 (2011)
  • Author(s): 横尾友隆、渡邊和寿、飯田薫子、鈴木浩明、島野仁、石川三衛、川上正舒、山田信博、岡崎康司、豊島秀男
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-05-19
• [Presentation] 膵β細胞増加作用を持つ新規消化管特異的分泌タンパクIBCAPの機能解析 (2011)
  • Author(s): 横尾友隆
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2011-05-19
• [Presentation] 新規消化管特異的分泌因子IBCAPの機能解析および糖尿病治療への可能性 (2011)
  • Author(s): 豊島秀男 [招待演者]、横尾友隆、鈴木浩明、石川三衛、川上正舒、島野仁、山田信博、岡崎康司
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-04-22
• [Presentation] 新規消化管特異的分泌因子IBCAPの機能解析および糖尿病治療への可能性 (2011)
  • Author(s): 豊島秀男
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 兵庫県神戸市(招待講演)
  • Year and Date: 2011-04-22
• [Presentation] インスリン分泌刺激作用を持つ新規消化管特異的分泌タンパクIBCAPの機能解析 (2010)
  • Author(s): 横尾友隆
  • Organizer: 第8回 RCGMフロンティア国際シンポジウム
  • Place of Presentation: 埼玉医科大学(埼玉県日高市)
  • Year and Date: 2010-11-03
• [Presentation] Transgenic mice expressing an intestine-specific secretory protein, IBCAP, demonstrates pancreatic beta cell augmenting activity (2010)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Ishikawa SE, Kawakami M, Yamada N, Okazaki Y, Toyoshima H
  • Organizer: 46th EASD Annual Meeting
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-22
• [Presentation] Transgenic mice expressing an intestine-specific secretory protein, IBCAP, demonstrates pancreatic beta cell augmenting activity. (2010)
  • Author(s): Tomotaka YOKOO
  • Organizer: 第46回 欧州糖尿病学会議(EASD)
  • Place of Presentation: STOCKHOLMSMASSAN(ストックホルム)
  • Year and Date: 2010-09-22
• [Presentation] Transgenic Mice Expressing an Intestine-Specific Secretory Protein, IBCAP (Formerly CF266), Demonstrates Pancreatic β-Cell Augmenting Activity (2010)
  • Author(s): Yokoo T, Watanabe K, Iida KT, Suzuki H, Shimano H, Ishikawa SE, Kawakami M, Yamada N, Toyoshima H
  • Organizer: ENDO 2010: The 92nd Annual Meeting & Expo
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-06-19
• [Presentation] Transgenic Mice Expressing an Intestine-Specific Secretory Protein, IBCAP (Formerly CF266), Demonstrates Pancreatic ss-Cell Augmenting Activity. (2010)
  • Author(s): Tomotaka YOKOO
  • Organizer: 第92回 米国内分泌学会年次集会(ENDO 2010)
  • Place of Presentation: San Diego Convention Center(サンディエゴ)
  • Year and Date: 2010-06-19
• [Patent(Industrial Property Rights)] 膵臓内分泌細胞の生産方法 (2013)
  • Inventor(s): 豊島秀男、岡崎康司、横尾友隆、菅原泉
  • Industrial Property Rights Holder: 埼玉医科大学
  • Filing Date: 2013-05-25
• [Patent(Industrial Property Rights)] 膵臓内分泌細胞の生産方法 (2012)
  • Inventor(s): 豊島秀男、岡﨑康司、横尾友隆、菅原泉
  • Industrial Property Rights Holder: 埼玉医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-120281
  • Filing Date: 2012-05-25