Identification of target genes in insulin signaling of arcuate nucleus by Translating Ribosome Affinity Purification (TRAP)

• Project/Area Number: 22590995
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Keio University
• Principal Investigator: NAKAE Jun 慶應義塾大学, 医学部, 特任准教授 (00344573)
• Co-Investigator(Kenkyū-buntansha): KAWANO Yoshinaga 慶應義塾大学, 医学部, 助教 (80571463)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エネルギー / 糖質代謝異常 / AGRPニューロン / TRAP法 / 遺伝子発現解析 / 糖尿病 / シグナル伝達 / マイクロアレイ / PDK1 / Foxo1 / 遺伝子発現 / リボソーム蛋白

Research Abstract

The purpose of this study is to analyze geneexpression profiling in a neuron-specific manner. We tried to establish Translating Ribosome Affinity Purification (TRAP) method. We tried to generate transgenic mice, in which the fusion protein of EGFP and N-t

Research Products

• [Journal Article] Novel repressor regulates insulin sensitivity through interaction with Foxo1 (2012)
  • Author(s): Nakae J, Cao Y, Hakuno F, Takemori H, Kawano Y, Sekioka R, Abe T, Kiyonari H, Tanaka T, Sakai J, Takahashi S, Itoh H
  • Journal Title: EMBO J Volume: 31 Pages: 2275-95
  • Peer Reviewed
• [Journal Article] Loss of PDK1-Foxo1 signaling in myeloid Cells predisposes to adipose tissue inflammation and insulin resistance. (2012)
  • Author(s): Kawano Y, Nakae J, Watanabe N, Fujisaka S, Iskandar K, Sekioka R, Hayashi Y, Tobe K, Kasuga M, Noda T, Yoshimura A, Onodera M, Itoh H
  • Journal Title: Diabetes Volume: 61 Pages: 1935-1948
  • Peer Reviewed
• [Journal Article] Overexpression of FoxO1 in the Hypothalamus and Pancreas Causes Obesity and Glucose Intolerance (2012)
  • Author(s): Kim HJ, Kobayashi M, Sasaki T, Kikuchi O, Amano K, Kitazumi T, Lee YS, Yokota-Hashimoto H, Susanti VY, Kitamura YI, Nakae J, Kitamura T
  • Journal Title: Endocrinology Volume: 153 Pages: 659-71
  • Peer Reviewed
• [Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice (2012)
  • Author(s): Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T, Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, K
  • Journal Title: PLoS One Volume: 7(5)
  • Peer Reviewed
• [Journal Article] Loss of PDK1-Foxo1 signaling in myeloid Cells predisposes to adipose tissue inflammation and insulin resistance (2012)
  • Author(s): Kawano Y, Nakae J, Watanabe N, Fujisaka S, Iskandar K, Sekioka R, Hayashi Y, Tobe K, Kasuga M, Noda T, Yoshimura A, Onodera M, Itoh H
  • Journal Title: Diabetes Volume: 61 Issue: 8 Pages: 1935-1948
  • DOI: 10.2337/db11-0770
  • Peer Reviewed
• [Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice (2012)
  • Author(s): Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, et al.
  • Journal Title: PLoS One Volume: 7 Issue: 5 Pages: e37803-e37803
  • DOI: 10.1371/journal.pone.0037803
  • Peer Reviewed
• [Journal Article] Overexpression of Foxo1 in the hypothalamus and pancreas causes obesity and glucose intolerance (2012)
  • Author(s): Kim HJ, Kobayashi M, Sasaki T, Kikuchi O, Amano K, Kitazumi T, Lee YS, Yokota-Hashimoto H, Susanti VY, Kitamura YI, Nakae J, Kitamura T
  • Journal Title: Endocrinology Volume: 153 Pages: 659-671
  • Peer Reviewed
• [Journal Article] PDK1-Foxo1 in Agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure (2011)
  • Author(s): Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y, Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H, Kangawa K, Itoh H, Noda T, Kasuga M, Nakae J
  • Journal Title: PLoS ONE Volume: 6 Issue: 4 Pages: 2100-2112
  • DOI: 10.1371/journal.pone.0018324
  • Peer Reviewed
• [Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. (2011)
  • Author(s): Shimizu N,Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, and Tanaka H.
  • Journal Title: Cell Metab Volume: 13 Pages: 170-82
  • Peer Reviewed
• [Journal Article] Constitutive Expression of Insulin Receptor Substrate (IRS)-1 Inhibits Myogenic Differentiation through Nuclear Exclusion of Foxo1 in L6 Myoblasts. (2011)
  • Author(s): Hakuno F, Yamauchi Y, Kaneko G, Yoneyama Y, Nakae J, ChidaK, KadowakiT, Yamanouchi K, NishiharaM, TakahashiSI.
  • Journal Title: PLoS ONE2011 Volume: 6(10)
  • Peer Reviewed
• [Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle (2011)
  • Author(s): Shimizu N, Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H
  • Journal Title: Cell Metab Volume: 13 Pages: 170-182
  • Peer Reviewed
• [Journal Article] Constitutive expression of insulin receptor substrate (IRS)-1 inhibits myogenic differentiation through nuclear exelusion of Foxo1 in L6 myoblasts (2011)
  • Author(s): Hakuno F, Yamauchi Y, Kaneko G, Yoneyama Y, Nakae J, Chida K, Kadowaki T, Yamanouchi K, Nishihara M, Takahashi S
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. (2011)
  • Author(s): Shimizu N, Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H.
  • Journal Title: Cell Metab Volume: 13 Pages: 170-182
  • Peer Reviewed
• [Journal Article] PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure. (2011)
  • Author(s): Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y, Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H, Kangawa K, Itoh H, Noda T, Kasuga M, Nakae J.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] PDK1/FoxO1 pathway in POMC neurons regulates Pomc expression and food intake. (2010)
  • Author(s): Iskandar K, Cao Y, Hayashi Y, Nakata M, Takano E, Yada T, Zhang C, Ogawa W M, Oki M, Chua S Jr, Itoh H, Noda T, Kasuga M, Nakae J.
  • Journal Title: Am J Physiol Endocrinol Metab Volume: 298
  • Peer Reviewed
• [Journal Article] Deacetylation of FoxO by Sirt1 plays an essential role in mediating starvation-induced autophagy in cardiac myocytes. (2010)
  • Author(s): Hariharan N, Maejima Y, Nakae J, Paik J, Depinho RA, Sadoshima J
  • Journal Title: Circ Res Volume: 107 Pages: 1470-82
  • Peer Reviewed
• [Journal Article] PDK1/FoxO1 pathway in POMC neurons regulates Pomc expression and food intake. (2010)
  • Author(s): Iskandar K, Cao Y, Hayashi Y, Nakata M, Takano E, Yada T, Zhang C, Ogawa W M, Oki M, Chua S Jr, Itoh H, Noda T, Kasuga M, Nakae J.
  • Journal Title: Am J Physiol Endocrinol Metab. Volume: 298
  • Peer Reviewed
• [Journal Article] Deacetylation of FoxO by Sirt1 plays an essential role in mediating starvation-induced autophagy in cardiac myocytes. (2010)
  • Author(s): Hariharan N, Maejima Y, Nakae J, Paik J, Depinho RA, Sadoshima J.
  • Journal Title: Circ Res. Volume: 107 Pages: 170-182
  • Peer Reviewed
• [Presentation] マウス膵β 細胞におけるFCoR/Foxo1 CoRepressorはインスリン分泌を調節しうる. (2012)
  • Author(s): 小谷紀子、中江 淳、川野義長、伊藤 裕
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-12
• [Presentation] マウス高脂肪食負荷による肝臓および腸管の慢性炎症に与える影響の検討. (2012)
  • Author(s): 川野義長、中江 淳、伊藤 裕
  • Organizer: 第33回日本肥満学会.
  • Place of Presentation: 京都
  • Year and Date: 2012-10-12
• [Presentation] 弓状核AgRPニューロン特異的PDK1欠損マウスの骨代謝異常 (2012)
  • Author(s): 中田正範、笹沼秀幸、中江 淳、矢田俊彦
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-11
• [Presentation] 糖・エネルギー代謝調節におけるNEWCOMERS. (2012)
  • Author(s): 中江 淳
  • Organizer: 第46回日本小児内分泌学会学術集会 教育セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-28
• [Presentation] 3-Phosphoinositide-Dependent Protein Kinase 1 (Pdk1) Regulates Macrophage Migration, Polarization, and Insulin Sensitivity. (2012)
  • Author(s): Kawano Y, Nakae J, Itoh H
  • Organizer: 72nd Scientific Sessions, American Diabetes Association.
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2012-06-11
• [Presentation] 新規Foxo1 CoRepressor (FCoR/P13)は膵α細胞において、インスリン分泌を調節しうる (2012)
  • Author(s): 中江 淳、小谷紀子、川野義長、伊藤 裕
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-17
• [Presentation] マクロファージにおける 3-phosphoinositide dependent protein kinase 1 (Pdk1)は生体内の糖代謝調節 に重要である (2012)
  • Author(s): 川野義長、中江 淳、春日雅人、伊藤 裕
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-17
• [Presentation] 脂肪組織マクロファージにおける3-phosphoinositide-dependent protein kinase 1 (Pdk1)の生理的役 割の検討 (2012)
  • Author(s): 川野義長、中江 淳、春日雅人、伊藤 裕
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-04-19
• [Presentation] oxo1 in Adipose Tissue Macrophages Induces Ccr2Expression and Insulin Resistance. (2011)
  • Author(s): Kawano Y, Sekioka R, Itoh H, Nakae J
  • Organizer: 71st Scientific Sessions, American Diabetes Association
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2011-06-24
• [Presentation] Foxo1 in Adipose Tissue Macrophages Induces Ccr2 Expression and Insulin Resistance (2011)
  • Author(s): Kawano Y, Sekioka R, Itoh H, Nakae J
  • Organizer: 71st Scientific Sessions, American Diabetes Association
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2011-06-24
• [Presentation] マクロファージにおけるFoxo1はCcr2発現を誘導しインスリン抵抗性を惹起する. (2011)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤 裕、中江 淳
  • Organizer: 第54回日本糖尿病学会年次学術集会.
  • Place of Presentation: 札幌、ロイトン札幌
  • Year and Date: 2011-05-21
• [Presentation] マクロファージにおけるFoxo1はCcr2発現を誘導しインスリン抵抗性を惹起する (2011)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤裕、中江淳
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌、ロイトン札幌
  • Year and Date: 2011-05-21
• [Presentation] 脂肪組織におけるFoxo1の過剰発現の糖・エネルギー代謝への影響の検討 (2011)
  • Author(s): 関岡理沙、川野義長、伊藤 裕、中江 淳
  • Organizer: 第54回日本糖尿病学会年次学術集会.
  • Place of Presentation: 札幌、ロイトン札幌
  • Year and Date: 2011-05-19
• [Presentation] 脂肪組織におけるFoxo1の過剰発現の糖・エネルギー代謝への影響の検討 (2011)
  • Author(s): 関岡理沙、川野義長、伊藤裕、中江淳
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌、ロイトン札幌
  • Year and Date: 2011-05-19
• [Presentation] 脂肪組織浸潤マクロファージにおけるFoxo1の役割の検討 (2011)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤 裕、中江 淳.
  • Organizer: 第84回日本内分泌学会学術総会.
  • Place of Presentation: 神戸、神戸国際会議場
  • Year and Date: 2011-04-22
• [Presentation] 脂肪組織におけるFoxo1の過剰発現は耐糖能悪化を惹起する. (2011)
  • Author(s): 関岡理沙、川野義長、伊藤 裕、中江 淳.
  • Organizer: 第84回日本内分泌学会学術総会.
  • Place of Presentation: 神戸、神戸国際会議場
  • Year and Date: 2011-04-22
• [Presentation] 脂肪組織浸潤マクロファージにおけるFoxo1の役割の検討 (2011)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤裕、中江淳
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸、神戸国際会議場
  • Year and Date: 2011-04-22
• [Presentation] 脂肪組織におけるFoxo1の過剰発現は耐糖能悪化を惹起する (2011)
  • Author(s): 関岡理沙、川野義長、伊藤裕、中江淳
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸、神戸国際会議場
  • Year and Date: 2011-04-22
• [Presentation] Overexpression of FoxO1 in adipose tissue macrophages induces insulin resistance. (2010)
  • Author(s): Nakae J, Kawano Y, Sekioka R, Fujisaka S, Tobe K, Itoh H.
  • Organizer: FASEB Summer Research Conferences. AMPK : Central Regulatory System in Metaholism & Growth.
  • Place of Presentation: 京都
  • Year and Date: 2010-10-04
• [Presentation] マクロファージにおけるFoxO1はCcr2発現を誘導しインスリン抵抗性を惹起しうる (2010)
  • Author(s): 川野 義長、関岡 理沙、藤坂 志帆、戸辺 一之、伊藤 裕、中江 淳
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋
  • Year and Date: 2010-10-02
• [Presentation] マクロファージにおけるFox01はCcr2発現を誘導しインスリン抵抗性を惹起しうる (2010)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤裕、中江淳
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋
  • Year and Date: 2010-10-02
• [Presentation] 脂肪組織マクロファージにおけるFoxO1の機能解析 (2010)
  • Author(s): 川野 義長、関岡 理沙、藤坂 志帆、戸辺 一之、伊藤 裕、中江 淳.
  • Organizer: 第15回アデイポサイエンス研究会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-21
• [Presentation] 脂肪組織マクロファージにおけるFox01の機能解析 (2010)
  • Author(s): 川野義長、関岡理沙、藤坂志帆、戸辺一之、伊藤裕、中江淳
  • Organizer: 第15回アデイポサイエンス研究会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-21
• [Presentation] ノックアウトマウスを用いたFoxO1結合蛋白P13の生理作用の検討. (2010)
  • Author(s): 中江 淳、川野 義長、関岡 理沙、伊藤 裕
  • Organizer: 第53回日本糖尿病学会年次学術集会.
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-27
• [Presentation] ノックアウトマウスを用いたFoxO1結合蛋白P13の生理作用の検討 (2010)
  • Author(s): 中江淳、川野義長、関岡理沙、伊藤裕
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-27
• [Presentation] 3-Phosphoinositide-Dependent Protein Kinase 1 (Pdk1) Regulates Macrophage Migration, Polarization, and Insulin Sensitivity
  • Author(s): Kawano Y, Nakae J, Itoh H
  • Organizer: 72nd Scientific Sessions, American Diabetes Association
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] 脂肪組織マクロファージにおける3-phosphoinositide-dependent protein kinase 1 (Pdk1)の生理的役割の検討
  • Author(s): 川野義長、中江 淳、春日雅人、伊藤 裕
  • Organizer: 第85回日本内分泌学会学術総会口演
  • Place of Presentation: 名古屋
• [Presentation] 新規Foxo1 CoRepressor (FCoR/P13)は膵α細胞において、インスリン分泌を調節しうる
  • Author(s): 中江 淳、小谷紀子、川野義長、伊藤 裕
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
• [Presentation] マクロファージにおける3-phosphoinositide dependent protein kinase 1 (Pdk1)は生体内の糖代謝調節に重要である
  • Author(s): 川野義長、中江 淳、春日雅人、伊藤 裕
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
• [Presentation] マウス膵β細胞におけるFCoR/Foxo1 CoRepressorはインスリン分泌を調節しうる
  • Author(s): 小谷紀子、中江 淳、川野義長、伊藤 裕
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
• [Presentation] マウス高脂肪食負荷による肝臓および腸管の慢性炎症に与える影響の検討
  • Author(s): 川野義長、中江 淳、伊藤 裕
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
• [Presentation] 弓状核AgRPニューロン特異的PDK1欠損マウスの骨代謝異常
  • Author(s): 中田正範、笹沼秀幸、中江 淳、矢田俊彦
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
• [Presentation] 糖・エネルギー代謝調節におけるNEWCOMERS
  • Author(s): 中江 淳
  • Organizer: 第46回日本小児内分泌学会学術集会 教育セミナー
  • Place of Presentation: 大阪
  • Invited
• [Book] インスリン作用とFoxo1.糖尿病ナビゲーター (2010)
  • Author(s): 中江淳、伊藤裕
  • Total Pages: 4
  • Publisher: メデイカルレビュー社