Identification of target genes in insulin signaling of arcuate nucleus by Translating Ribosome Affinity Purification (TRAP)
Project/Area Number |
22590995
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Keio University |
Principal Investigator |
NAKAE Jun 慶應義塾大学, 医学部, 特任准教授 (00344573)
|
Co-Investigator(Kenkyū-buntansha) |
KAWANO Yoshinaga 慶應義塾大学, 医学部, 助教 (80571463)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | エネルギー / 糖質代謝異常 / AGRPニューロン / TRAP法 / 遺伝子発現解析 / 糖尿病 / シグナル伝達 / マイクロアレイ / PDK1 / Foxo1 / 遺伝子発現 / リボソーム蛋白 |
Research Abstract |
The purpose of this study is to analyze geneexpression profiling in a neuron-specific manner. We tried to establish Translating Ribosome Affinity Purification (TRAP) method. We tried to generate transgenic mice, in which the fusion protein of EGFP and N-t
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Novel repressor regulates insulin sensitivity through interaction with Foxo12012
Author(s)
Nakae J, Cao Y, Hakuno F, Takemori H, Kawano Y, Sekioka R, Abe T, Kiyonari H, Tanaka T, Sakai J, Takahashi S, Itoh H
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Journal Title
EMBO J
Volume: 31
Pages: 2275-95
Related Report
Peer Reviewed
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[Journal Article] Loss of PDK1-Foxo1 signaling in myeloid Cells predisposes to adipose tissue inflammation and insulin resistance.2012
Author(s)
Kawano Y, Nakae J, Watanabe N, Fujisaka S, Iskandar K, Sekioka R, Hayashi Y, Tobe K, Kasuga M, Noda T, Yoshimura A, Onodera M, Itoh H
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Journal Title
Diabetes
Volume: 61
Pages: 1935-1948
Related Report
Peer Reviewed
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[Journal Article] Overexpression of FoxO1 in the Hypothalamus and Pancreas Causes Obesity and Glucose Intolerance2012
Author(s)
Kim HJ, Kobayashi M, Sasaki T, Kikuchi O, Amano K, Kitazumi T, Lee YS, Yokota-Hashimoto H, Susanti VY, Kitamura YI, Nakae J, Kitamura T
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Journal Title
Endocrinology
Volume: 153
Pages: 659-71
Related Report
Peer Reviewed
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[Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice2012
Author(s)
Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T, Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, K
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Loss of PDK1-Foxo1 signaling in myeloid Cells predisposes to adipose tissue inflammation and insulin resistance2012
Author(s)
Kawano Y, Nakae J, Watanabe N, Fujisaka S, Iskandar K, Sekioka R, Hayashi Y, Tobe K, Kasuga M, Noda T, Yoshimura A, Onodera M, Itoh H
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Journal Title
Diabetes
Volume: 61
Issue: 8
Pages: 1935-1948
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice2012
Author(s)
Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, et al.
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Journal Title
PLoS One
Volume: 7
Issue: 5
Pages: e37803-e37803
DOI
Related Report
Peer Reviewed
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[Journal Article] Overexpression of Foxo1 in the hypothalamus and pancreas causes obesity and glucose intolerance2012
Author(s)
Kim HJ, Kobayashi M, Sasaki T, Kikuchi O, Amano K, Kitazumi T, Lee YS, Yokota-Hashimoto H, Susanti VY, Kitamura YI, Nakae J, Kitamura T
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Journal Title
Endocrinology
Volume: 153
Pages: 659-671
Related Report
Peer Reviewed
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[Journal Article] PDK1-Foxo1 in Agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure2011
Author(s)
Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y, Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H, Kangawa K, Itoh H, Noda T, Kasuga M, Nakae J
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Journal Title
PLoS ONE
Volume: 6
Issue: 4
Pages: 2100-2112
DOI
Related Report
Peer Reviewed
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[Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.2011
Author(s)
Shimizu N,Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, and Tanaka H.
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Journal Title
Cell Metab
Volume: 13
Pages: 170-82
Related Report
Peer Reviewed
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[Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle2011
Author(s)
Shimizu N, Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H
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Journal Title
Cell Metab
Volume: 13
Pages: 170-182
Related Report
Peer Reviewed
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[Journal Article] Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.2011
Author(s)
Shimizu N, Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H.
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Journal Title
Cell Metab
Volume: 13
Pages: 170-182
Related Report
Peer Reviewed
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[Journal Article] PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure.2011
Author(s)
Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y, Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H, Kangawa K, Itoh H, Noda T, Kasuga M, Nakae J.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] PDK1/FoxO1 pathway in POMC neurons regulates Pomc expression and food intake.2010
Author(s)
Iskandar K, Cao Y, Hayashi Y, Nakata M, Takano E, Yada T, Zhang C, Ogawa W M, Oki M, Chua S Jr, Itoh H, Noda T, Kasuga M, Nakae J.
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Journal Title
Am J Physiol Endocrinol Metab
Volume: 298
Related Report
Peer Reviewed
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[Journal Article] PDK1/FoxO1 pathway in POMC neurons regulates Pomc expression and food intake.2010
Author(s)
Iskandar K, Cao Y, Hayashi Y, Nakata M, Takano E, Yada T, Zhang C, Ogawa W M, Oki M, Chua S Jr, Itoh H, Noda T, Kasuga M, Nakae J.
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Journal Title
Am J Physiol Endocrinol Metab.
Volume: 298
Related Report
Peer Reviewed
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