Generation of gene targeted rat models with obesity and lipodystrophy for elucidating the mechanism of leptin action
| Project/Area Number |
22591010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kyoto University |
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Principal Investigator |
ABE Megumi 京都大学, 医学研究科, 助教 (20568688)
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| Co-Investigator(Kenkyū-buntansha) |
EBIHARA Ken 京都大学, 医学研究科, 准教授 (70362514)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖尿病 / レプチン / 動物モデル / 遺伝子変異ラット / セイピン / PPARγ |
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| Research Abstract |
We generated and characterized a leptin deficient Lep^<mkyo>/Lep^<mkyo> rat that had a nonsense mutation in leptin gene and seipin knockout rat by the method using ENU mutagenesis. TZDs differentially regulated hepatic peroxisome proliferator-activated receptor gamma (PPARg)mRNA expression and body fat distribution between Lep^<ob>/Lep^<ob> mice and Lepmkyo/Lepmkyorats. Weidentified 8 genes those primarily respond to leptin in the liver commonly in rat and mouse. We detected that seipin deficient rat were infertile due to azoospermia and their spatial working memory is impairedalong with brain weight decrease
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Report
(4 results)
Research Products
(37 results)
