Snapshot peptidomics for bioactive peptide discovery
| Project/Area Number |
22591023
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
SASAKI Kazuki 独立行政法人国立循環器病研究センター, 研究所, 室長 (80260313)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 質量分析 / 生理活性ペプチド / ペプチドミクス / 内分泌学 / 神経内分泌腫瘍 / 視床下部 / 分泌顆粒 / 内分泌腫瘍 |
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| Research Abstract |
A new strategy for discovering biologically active peptides has been developed. It involves mass spectrometry-based profiling of secretory peptides released from cells with secretory granules. Selected human cell lines of endocrine origin were analyzed for their secretory peptide profiles using liquid chromatography-tandem mass spectrometry. A large set of secretory peptide sequences was used to identify processing sites of secretory proteins. Candidates for biologically active peptides were selected from the peptide processing maps and tested on selected bioassay systems. This approach has resulted in the identification of previously uncharacterized biologically active peptides, designated NERP-3 and NERP-4, which raise intracellular calcium levels in hypothalamus and pituitary tissues. Furthermore, it was found that NERP-3 stimulates vasopressin release from an in vitro preparation of the hypothalamic supraoptic nucleus, where vasopressin neurons reactive to NERP-3 were identified. This peptidomics approach has the potential to identify biologically active peptides that are beyond the reach of conventional strategies.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro.2012
Author(s)
Moin AS, Yamaguchi H, Rhee M, Kim JW, Toshinai K, Zaved Waise TM, Kaznin F, Matsuo T, Sasaki K, Minamino N, Yoon KH, Nakazato M.
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Journal Title
Biochem Biophys Res Commun.
Volume: 428
Pages: 512-7
Related Report
Peer Reviewed
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[Journal Article] Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro.2012
Author(s)
Moin AS, Yamaguchi H, Rhee M, Kim JW, Toshinai K, Waise TM, Naznin F, Matsuo T, Sasaki K, Minamino N, Yoon KH, Nakazato M
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Journal Title
Biochem Biophys Res Commun
Volume: 428
Issue: 4
Pages: 512-517
DOI
Related Report
Peer Reviewed
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[Journal Article] Distribution of neuroendocrine regulatory peptide-1 and -2, and proteolytic processing of their precursor VGF protein in the rat.2010
Author(s)
Mishiro-Sato E, Sasaki K, Matsuo T, Kageyama H, Yamaguchi H, Date Y, Matsubara M, Ishizu T, Yoshizawa-Kumagaye K, Satomi Y, Takao T, Shioda S, Nakazato M, Minamino N.
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Journal Title
J Neurochem
Volume: 114
Pages: 1097-106
Related Report
Peer Reviewed
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