Role for γδT lymphocytes in autoimmune bone marrow failure syndrome
| Project/Area Number |
22591024
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAWADA Kenichi 秋田大学, 医学部, 教授 (90226069)
FUJISHIMA Naohito 秋田大学, 医学部, 講師 (70422152)
FUJISHIMA Masumi 秋田大学, 医学部, 臨床系医員 (80422153)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 赤芽球癆 / 後天性 / 慢性 / γδT細胞 / CDR3 / 骨髄不全症候群 |
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| Research Abstract |
We examined the γδ T cell receptor repertoire in 19 pure red cell aplasia (PRCA) patients and found that there was a difference in γδ T-cell repertoires between PRCA patients and healthy donors. We observed an increase in Vδ1 γδ T cells and a decrease in Vδ2 T cells in PRCA patients. An increase of Vδ1 γδ T cells was not found in aplastic anemia patients. CDR3.1 size distribution patterns were skewed in 9 out of 13 PRCA patients examined, although the skewing was also observed in 7 out of 10 healthy individuals. No significant changes were present in CDR3.1 size distribution between PRCA patients and healthy donors. Moreover, no apparent consensus amino acid motifs were identified in PRCA patients. Expansion of Vδ1 T cells and depletion of Vδ2 T cells are unique features for chronic acquired PRCA but expansion of Vδ1 T cells does not seem to be the consequence of CDR3-dependent selection. We conclude that clonal expansion of Vδ1 T cells is not a general feature for chronic acquired PRCA.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] CDR3-independent expansion of Vδ1 T lymphocytes in acquired chronic pure red cell aplasia2013
Author(s)
Michishita Y, Hirokawa M, Fujisima N, Abe Y, Fujishima M, Guo Y-M, Ubukawa K, Liu J, Yoshioka T, Kameoka Y, Saitoh H, Tagawa H, Takanashi N, Sawada K
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Journal Title
Immunol Lett
Volume: 150
Pages: 23-29
Related Report
Peer Reviewed
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[Journal Article] Enucleation of human erythroblasts involves nonmuscle myosin IIB2012
Author(s)
Ubukawa K, Guo YM, Takahashi M, Hirokawa M, Michishita Y, Nara M, Tagawa H, Takahashi N, Komatsuda A, Nunomura W, Takakuwa Y, Sawada K
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Journal Title
Blood
Volume: 119
Pages: 1036-1044
Related Report
Peer Reviewed
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[Journal Article] Age-associated alteration of γδ T cell repertoire and different profiles of activation-induced death of V1 and Vδ2 T cells.2011
Author(s)
Michishita Y, Hirokawa M, Guo YM, Abe Y, Liu J, Ubukawa K, Fujishima N, Fujishima M, Yoshioka, T Kameoka Y, Saito H, Tagawa H, Takahashi N, Sawada K
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Journal Title
Int J Hematol
Volume: 94
Pages: 230-240
Related Report
Peer Reviewed
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[Journal Article] CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells2010
Author(s)
Guo YM, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao W, Sawada K
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Journal Title
Blood
Volume: 115
Pages: 4569-4579
Related Report
Peer Reviewed
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[Journal Article] Delayed addition of tumor necrosis factor (TNF) antagonists inhibits the generation of CD11c+ dendritic cells derived from CD34+ cells exposed to TNF-α.2010
Author(s)
Guo YM, Hirokawa M, Takahashi N, Fujishima M, Fujishima N, Komatsuda A, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Hebiguchi M, Xiao W, Sawada K
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Journal Title
Int J Hematol
Volume: 91
Pages: 61-68
NAID
Related Report
Peer Reviewed
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[Presentation] Efficacy and long-term outcome of intervention for pure red cell aplasia (PRCA) following allogeneic hematopoietic stem cell transplantation from major ABO-incompatible donors2011
Author(s)
Hirokawa M, Sawada K, Fujishima N, Fukuda T, Akiyama H, Imamura M, Adachi S, Urabe A, Okamoto S, Suzuki R, Tanimoto M, Nakao S, ugita M, Morishima Y, Sakamaki H, Ozawa K
Organizer
16th Congress of European Hematology Association
Place of Presentation
London
Related Report
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[Presentation] CDR3-independent expansion of Vδ1 γδ T lymphocytes and depletion of Vδ2 T cells are unique features in acquired pure red cell aplasia2011
Author(s)
Michishita Y, Hirokawa M, Fujishima N, Abe Y, Fujishima M, Guo Y-M, Ubukawa K, Liu J, Yoshioka T, Kameoka Y, Saitoh H, Tagawa H, Takahashi N, Sawada K
Organizer
53rd American Society of Hematology Annual Meeting and Exposition
Place of Presentation
San Diego
Related Report
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[Presentation] Alteration of γδ T-cell repertoire in acquired chronic pure red cell aplasia2010
Author(s)
Hirokawa M, Michishita Y, Fujishima N, Fujishima M, Guo YM, Kameoka Y, Saitoh H, Tagawa H, Takahashi N, Sawada K
Organizer
1^<st> JSH International Symposium 2010
Place of Presentation
Akita, Japan
Related Report
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[Presentation] CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells2010
Author(s)
Guo Y-M, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao WG, Sawada K
Organizer
15^<th> Congress of the European Hematology Association
Place of Presentation
Barcelona
Related Report
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