Generation of tumor-bearing humanized mice that express human cytokine

• Project/Area Number: 22591027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: National Hospital Organization Nagoya Medical Center
• Principal Investigator: SAITO Toshiki 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), 名古屋医療センター臨床研究センター, 室長 (50557238)
• Research Collaborator: KUMAGAI Katsuyoshi 東京医科大学, 医学部, 助教 (20567911) ; HIRANO Naoto トロント大学, 免疫学, 准教授
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌 / 動物 / 免疫学

Research Abstract

First, we obtained fertilized eggs efficiently by means of in vitro fertilization (IVF); then, we attempted to generate CAG-EGFP Tg mice on an NOD/SCID background, finding that delayed timing of the microinjection after the IVF improved the time to development of the two-cell-stage embryos and the obtainment of newborns. We successfully generated Tg mice and confirmed the germ-line transmission in the offspring. In conclusion, we established a novel system for directly generating transgenic mice on an NOD/SCID background.

Research Products

• [Journal Article] Notch-dependent and -independent regulation of mature B cell lineage fate and humoral immune response by the LRF transcription factor homodimer (2011)
  • Author(s): Nagisa Sakurai
  • Journal Title: Journal of Clinical Investigation Volume: 121 Issue: 7 Pages: 2583-2598
  • DOI: 10.1172/jci45682
  • Peer Reviewed
• [Journal Article] In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. (2011)
  • Author(s): Fujisaki J, Wu J, Carlson AL, Silberstein L, Putheti P, Larocca R, Gao W, Saito TI, Lo Celso C, Tsuyuzaki H, Sato T, Cote D, Sykes M, Strom TB, Scadden DT, Lin CP
  • Journal Title: Nature Volume: 474 Issue: 7350 Pages: 216-219
  • DOI: 10.1038/nature10160
  • Peer Reviewed
• [Journal Article] Generation of Transgenic Mice on an NOD/SCID Background Using the Conventional Microinjection Technique. (2011)
  • Author(s): Saito TI, Kumagai K, Kubota N, Sasako T, Takizawa R, Sudo K, Kurokawa M, Kadowaki T.
  • Journal Title: Biol Reprod Volume: 84(4) Issue: 4 Pages: 682-8
  • DOI: 10.1095/biolreprod.110.087106
  • Peer Reviewed
• [Journal Article] Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine. (2011)
  • Author(s): Sakata-Yanagimoto M, Sakai T, Miyake Y, Saito TI, Maruyama H, Morishita Y, Nakagami-Yamaguchi E, Kumano K, Yagita H, Fukayama M, Ogawa S, Kurokawa M, Yasutomo K, Chiba S.
  • Journal Title: Blood. Volume: 117 Pages: 128-134
  • Peer Reviewed
• [Journal Article] Persistence of donor-derived protein in host myeloid cells after induced rejection of engrafted allogeneic bone marrow cells. (2010)
  • Author(s): Saito TI, Fujisaki J, Carlson AL, Lin CP, Sykes M.
  • Journal Title: Exp Hematol. Volume: 38 Pages: 333-339
  • Peer Reviewed
• [Journal Article] Invariant NKT cells are required for antitumor responses induced by host-versus-graft responses. (2010)
  • Author(s): Saito TI, Li HW, Sykes M.
  • Journal Title: J Immunol. Volume: 185 Pages: 2099-2105
  • Peer Reviewed