Generation of tumor-bearing humanized mice that express human cytokine
Project/Area Number |
22591027
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Hematology
|
Research Institution | National Hospital Organization Nagoya Medical Center |
Principal Investigator |
SAITO Toshiki 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), 名古屋医療センター臨床研究センター, 室長 (50557238)
|
Research Collaborator |
KUMAGAI Katsuyoshi 東京医科大学, 医学部, 助教 (20567911)
HIRANO Naoto トロント大学, 免疫学, 准教授
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 癌 / 動物 / 免疫学 |
Research Abstract |
First, we obtained fertilized eggs efficiently by means of in vitro fertilization (IVF); then, we attempted to generate CAG-EGFP Tg mice on an NOD/SCID background, finding that delayed timing of the microinjection after the IVF improved the time to development of the two-cell-stage embryos and the obtainment of newborns. We successfully generated Tg mice and confirmed the germ-line transmission in the offspring. In conclusion, we established a novel system for directly generating transgenic mice on an NOD/SCID background.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche.2011
Author(s)
Fujisaki J, Wu J, Carlson AL, Silberstein L, Putheti P, Larocca R, Gao W, Saito TI, Lo Celso C, Tsuyuzaki H, Sato T, Cote D, Sykes M, Strom TB, Scadden DT, Lin CP
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Journal Title
Nature
Volume: 474
Issue: 7350
Pages: 216-219
DOI
Related Report
Peer Reviewed
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[Journal Article] Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine.2011
Author(s)
Sakata-Yanagimoto M, Sakai T, Miyake Y, Saito TI, Maruyama H, Morishita Y, Nakagami-Yamaguchi E, Kumano K, Yagita H, Fukayama M, Ogawa S, Kurokawa M, Yasutomo K, Chiba S.
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Journal Title
Blood.
Volume: 117
Pages: 128-134
Related Report
Peer Reviewed
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