DNA methylation regulates self-renewal potential in hematopoietic stem cells

• Project/Area Number: 22591029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Keio University (2011-2012); The University of Tokyo (2010)
• Principal Investigator: EMA Hideo 慶應義塾大学, 医学部, 特任准教授 (50344445)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 造血幹細胞 / 発生 / 血液内科学 / 発生分化 / DNAメチル化 / Dnmt3b / 自己複製 / 発生・分化 / エピゲノム / 幹細胞 / 分化 / Dnmt3a

Research Abstract

To study a role of DNA methylation in stem cell development, we examined hematopoietic stem cells (HSCs) in livers from fetal mice deficient in de novo DNA methyltransferase 3a (Dnmt3a) or Dnmt3b. We showed that Dnmt3b but not Dnmt3a is essential for esta

Research Products

• [Journal Article] Two anatomically distinct niches regulate stem cell activity (2012)
  • Author(s): Ema, H., and Suda, T
  • Journal Title: Blood 120 Volume: 120 Issue: 11 Pages: 2174-2181
  • DOI: 10.1182/blood-2012-04-424507
  • Peer Reviewed
• [Journal Article] Homeostasis of hematopoietic stem cells regulated by the myelop roliferative disease associated-gene product Lnk/Sh2b3 via Bcl-xL (2012)
  • Author(s): Suzuki N, Yamazaki S, et al
  • Journal Title: Exp Hematol Volume: 40 Issue: 2 Pages: 166-174
  • DOI: 10.1016/j.exphem.2011.11.003
  • Peer Reviewed
• [Journal Article] Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche (2011)
  • Author(s): Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H
  • Journal Title: Cell Volume: 147 Issue: 5 Pages: 1146-1158
  • DOI: 10.1016/j.cell.2011.09.053
  • Peer Reviewed
• [Journal Article] Functional characterization of hematopoietic stem cells in the spleen (2011)
  • Author(s): Morita, Y., Iseki, A., Okamura, S., Suzuki, S., Nakauchi, H., and Ema, H.
  • Journal Title: Exp Hematol Volume: 39 Issue: 3 Pages: 351-359
  • DOI: 10.1016/j.exphem.2010.12.008
  • Peer Reviewed
• [Journal Article] Functional characterization of hematopoietic stem cells in the spleen (2011)
  • Author(s): Morita, Y., et al.
  • Journal Title: Exp Hematol Volume: 39 Pages: 351-359
  • Peer Reviewed
• [Journal Article] Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment (2010)
  • Author(s): Morita, Y., Ema, H., and Nakauchi, H
  • Journal Title: J Exp Med Volume: 207 Pages: 1173-1182
  • URL: http://jem.rupress.org/
  • Peer Reviewed
• [Journal Article] Mice lacking Dok-1, Dok-2, and Dok-3 succumb to aggressive histiocytic sarcoma (2010)
  • Author(s): Mashima, R., Honda, K., Yang, Y., Morita, Y., Inoue, A., Arimura, S., Nishina, H., Ema, H., Nakauchi, H., Seed, B., Oda, H., and Yamanashi, Y.
  • Journal Title: Lab Invest Volume: 90 Pages: 1357-1364
  • URL: http://www.nature.com/labinvest/index.html
  • Peer Reviewed
• [Presentation] Megakaryocyte Lineage Commitment in Hematopoietic Stem Cells (2011)
  • Author(s): Yamamoto M, Morita Y, Ema H, Ooehara J, Hamanaka S, Onodera M, Nakauchi H
  • Organizer: ASH annual meeting, San Diego
  • Place of Presentation: USA
  • Year and Date: 2011-12-13
• [Presentation] Megakaryocyte Lineage Commitment in Hematopoietic Stem Cells (2011)
  • Author(s): Yamamoto M, Morita Y, et al
  • Organizer: ASH annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-12-13
• [Presentation] Dnmt3b but not Dnmt3a is essential for establishment of self-renewal potential in developing hematopoietic stem cells (2011)
  • Author(s): Ema H
  • Organizer: USA-Japan Cancer Research Workshop
  • Place of Presentation: Hayama
  • Year and Date: 2011-02-25
• [Presentation] 造血幹細胞とiPS細 胞 (2010)
  • Author(s): 依馬秀夫、大津真、中内啓光
  • Organizer: 第31回 日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-05
• [Presentation] 造血幹細胞とiPS細胞 (2010)
  • Author(s): 依馬秀夫, 他
  • Organizer: 第31回日本再生・炎症医学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2010-08-05
• [Book] 再生医学叢書1「幹細胞」 (2012)
  • Author(s): 依馬秀夫 造血幹細胞
  • Publisher: 朝倉書店
• [Book] 造血幹細胞 再生医学叢書１「幹細胞」 (2012)
  • Author(s): 依馬秀夫
  • Publisher: 朝倉書店
• [Book] Principles of Hematopoietic Stem Cell Biology (2010)
  • Author(s): Ema H., et al.
  • Publisher: Human Press
• [Remarks]
  • URL: http://www.celldiff.med.keio.ac.jp/
• [Remarks] 慶應義塾大学医学部発生分化生物学
  • URL: http://www.celldiff.med.keio.ac.jp/
• [Remarks]
  • URL: http://www.ims.u-tokyo.acjp/imsut/jp/lab/stemcell/