Analysis of the regulatory mechanism for differentiation and functionof macrophages using a novel cytokine IL-34

• Project/Area Number: 22591041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kumamoto University
• Principal Investigator: SUZU Shinya 熊本大学, エイズ学研究センター, 准教授 (80363513)
• Research Collaborator: CHIHARA Takashi 熊本大学, エイズ学研究センター, 大学院生
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マクロファージ / 分化 / サイトカイン / 血液細胞分化

Research Abstract

Macrophages are important hematopoietic immune cells that regulateinnate immunity, and their differentiation and function are regulated by functionalproteins called cytokines. A major cytokine identified so far is M-CSF, but it has beenrecently found that another cytokine IL-34 also regulates macrophage differentiation andfunction. In this study, we revealed that these two cytokines differentially regulatedmacrophages, suggesting that M-CSF and IL-34 have different in vivo role

Research Products

• [Journal Article] Characteristics ofIFITM, the newly identifiedIFN-inducible anti-HIV-1 familyproteins (2013)
  • Author(s): Chutiwitoonchai N, Hiyoshi M,Hiyoshi-Yoshidomi Y, Hashimoto M,Tokunaga K, Suzu S.
  • Journal Title: Microbes and Infection Volume: 15 Pages: 280-290
  • Peer Reviewed
• [Journal Article] Importance of direct macrophage-tumorinteraction on progression of humanglioma (2012)
  • Author(s): Komohara Y, Hasita H, Ohnishi K,Fujiwara Y, Bai B, Nakagawa T, Suzu S,Nakamura H, Kuratsu J, Takeya M
  • Journal Title: Cancer Science Volume: 103 Pages: 2165-2172
  • Peer Reviewed
• [Journal Article] HIV-1 Proteins PreferentiallyActivate Anti-Inflammatory M2-TypeMacrophages (2012)
  • Author(s): Chihara T, Hashimoto M, Osman A,Hiyoshi-Yoshidomi Y, Suzu I,Chutiwitoonchai N, Hiyoshi M, Okada S,Suzu S
  • Journal Title: Journal of Immunology Volume: 188 Pages: 3620-3627
  • Peer Reviewed
• [Journal Article] HIV-1 Nef perturbs the function,structure, and signaling of the Golgithrough the Src kinase Hck (2012)
  • Author(s): Hiyoshi M, Takahashi-Makise N,Yoshidomi Y, Chutiwitoonchai N, ChiharaT, Okada M, Nakamura N, Okada S, Suzu S
  • Journal Title: Journal ofCellular Physiology Volume: 227 Pages: 1090-1097
  • Peer Reviewed
• [Journal Article] HIV-1 proteins preferentially activate anti-inflammatory M2-type macrophages (2012)
  • Author(s): Chihara T
  • Journal Title: Journal of Immunology Volume: 188 Issue: 8 Pages: 3620-3627
  • DOI: 10.4049/jimmunol.1101593
  • Peer Reviewed
• [Journal Article] Theidentification of a small moleculecompound that reduces HIV-1Nef-mediated viral infectivityenhancement (2011)
  • Author(s): Chutiwitoonchai N, Hiyoshi M, Mwimanzi P, Ueno T, Adachi A, Ode H, Sato H,Fackler OT, Okada S, Suzu S
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Effects ofnaturally-arising HIV Nef mutations oncytotoxic T lymphocytes recognition andNef's functionality in primarymacrophages (2011)
  • Author(s): Mwimanzi P, Hasan Z, Hassan R, Suzu S,Takiguchi M, Ueno T
  • Journal Title: Retrovirology Volume: 8 Pages: 50-50
  • Peer Reviewed
• [Journal Article] Macrophage infiltration and itsprognostic relevance in clear cell renalcell carcinoma (2011)
  • Author(s): Komohara Y, Hasita H, Ohnishi K,Fujiwara Y, Suzu S, Eto M, Takeya M
  • Journal Title: Cancer Science Volume: 102 Pages: 1424-1431
  • Peer Reviewed
• [Journal Article] Comparative study of humanhematopoietic cell engraftment intoBALB/c and C57BL/6 strain of rag-2/jak3double-deficient mice (2011)
  • Author(s): Ono A, Hattori S, Kariya R, Iwanaga S,Taura M, Harada H, Suzu S, Okada S
  • Journal Title: Journal ofBiomedicine and Biotechnology Volume: 2011 Pages: 539748-539748
  • Peer Reviewed
• [Journal Article] Small moleculeinhibition of HIV-1-induced MHC-Idownregulation identifies a temporallyregulated switch in Nef action (2010)
  • Author(s): Dikeakos JD, Atkins KM, Thomas L,Emert-Sedlak L, Byeon IL, Jung J, Ahn J,Wortmann MD, Kukull B, Saito M, KoizumiH, Williamson DM, Hiyoshi M, Barklis E,Takiguchi M, Suzu S, Gronenborn AM,Smithgall TE, Thomas G
  • Journal Title: Molecular Biology of the Cell Volume: 21 Pages: 3279-3292
  • Peer Reviewed
• [Journal Article] IL-34 andM-CSF share the receptor Fms but are notidentical in biological activity andsignal activation (2010)
  • Author(s): Chihara T, Suzu S (correspondence),Hassan R, Chutiwitoonchai N, Hiyoshi M,Motoyoshi K, Kimura F, Okada S
  • Journal Title: Cell Death andDifferentiation Volume: 17 Pages: 1917-1927
  • Peer Reviewed
• [Journal Article] Cepharanthine exertsantitumor activity oncholangiocarcinoma by inhibiting (2010)
  • Author(s): Seubwai W, Vaeteewoottacham K, HiyoshiM, Suzu S, Puapairoj A, Wongkham C, OkadaS, Wongkham S
  • Journal Title: Cancer Science Volume: 101 Pages: 1590-1595
  • Peer Reviewed
• [Journal Article] Hybridliposomes inhibit the growth ofcholangiocarcinoma by induction of cellcycle arrest in G1 phase (2010)
  • Author(s): Towata T, Komizu Y, Kariya R, Suzu S,Matsumoto Y, Kobayashi N, Wongkham C,Wongkham S, Ueoka R, Okada S
  • Journal Title: Bioorganic &Medicinal Chemistry Letters Volume: 20 Pages: 3680-3682
  • Peer Reviewed
• [Journal Article] Thera -irradiationin a primary effusion lymphoma mousemodel (2010)
  • Author(s): Shiraishi Y, Gotoh K, Towata T,Shimasaki T, Suzu S, Kojima A, Okada S
  • Journal Title: Experimental and TherapeuticMedicine Volume: 1 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Highly selective fusion andaccumulation of hybrid liposomes intoprimary effusion lymphoma cells alongwith induction of apoptosis (2010)
  • Author(s): Towata T, Komizu Y, Suzu S, Ueoka R,Okada S
  • Journal Title: Biochemical and Biophysical ResearchCommunications Volume: 393 Pages: 445-448
  • Peer Reviewed
• [Journal Article] Hybrid liposomesinhibit the growth of primary effusionlymphoma in vitro and in vivo (2010)
  • Author(s): Towata T, Komizu Y, Suzu S, MatsumotoY, Ueoka R, Okada S
  • Journal Title: LeukemiaResearch Volume: 34 Pages: 34-34
  • Peer Reviewed
• [Journal Article] Chronic hepatitis C virus infectionreduces NK cell frequency and suppressescytokine secretion: Reversion byanti-viral treatment (2010)
  • Author(s): Dessouki O, Kamiya Y, Nagahama H,Tanaka M, Suzu S, Sasaki Y, Okada S
  • Journal Title: Biochemical andBiophysical Research Communications Volume: 393 Pages: 445-448
  • Peer Reviewed
• [Journal Article] IL-34 and M-CSF share the receptor Fms but are not identical in biological activity and signal activation. (2010)
  • Author(s): Chihara T, et al
  • Journal Title: Cell Death and Differentiation Volume: 17 Pages: 1917-1927
  • Peer Reviewed
• [Presentation] HIV-1 drives differentiated macrophages toward a pro-inflammatory state (2011)
  • Author(s): Shinya Suzu
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-15
• [Presentation] Different activity and downstream signaling of IL-34 and M-CSF, which share the receptor Fms (2010)
  • Author(s): Suzu S
  • Organizer: 18^<th> International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: Parea Hall, Kumamoto
  • Year and Date: 2010-05-21
• [Remarks]
  • URL: http://www.caids.kumamoto-u.ac.jp/data/suzu/index.html