Analysis of the iron recycling mechanism in the degradation and production process of red blood cell

• Project/Area Number: 22591052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kawasaki Medical School
• Principal Investigator: KISHI Fumio 川崎医科大学, 医学部, 教授 (40153077)
• Co-Investigator(Kenkyū-buntansha): YANATORI Izumi 川崎医科大学, 医学部, 助教 (40454847)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 血液内科学 / 赤血球 / ヘム / 鉄 / FLVCR1 / 輸送体 / PCARP / 二価鉄 / DMT1

Research Abstract

We analyzed the localizations of heme transportes in polarized cells, and found that HCP1 has a role in the uptake of dietary heme. We found that 16Leu at N-terminal cytosolic domain of DMT1A-I was crucial for its plasma membrane localization. Moreover, we analyzed the function of the FLVCR1 mutants identified in posterior column ataxia and retinitis pigmentosa (PACARP) patients and found that their loss of heme export activity is caused by their missorting in the cell.

Research Products

• [Journal Article] Mutations of FLVCR1 in posterior column ataxia and retinitis pigmentosa result in the loss of heme export activity (2012)
  • Author(s): Yanatori I, Yasui Y, Miura K, Kishi F
  • Journal Title: Blood Cells Mol Dis Volume: vol.49、No.1 Issue: 1 Pages: 60-66
  • DOI: 10.1016/j.bcmd.2012.03.004
  • Peer Reviewed
• [Journal Article] Heme and non-heme iron transporters in non-polarized andpolarized cells (2010)
  • Author(s): Yanatori I, Tabuchi M, Kawai Y, YasuiY, Akagi R, Kishi F
  • Journal Title: BMC Cell Biol. Volume: Vol.11 Issue: 1 Pages: 39-39
  • DOI: 10.1186/1471-2121-11-39
  • Peer Reviewed
• [Journal Article] Heme and non-heme iron transporters in non-polarized and polarized cells (2010)
  • Author(s): Yanatori.I
  • Journal Title: BMC cell biology Volume: 11 Pages: 39-39
  • Peer Reviewed
• [Journal Article] Liver international : official journal of the International Association for the Study of the Liver (2010)
  • Author(s): Nishina S.
  • Journal Title: Liver international Volume: 30 Pages: 683-692
  • Peer Reviewed
• [Journal Article] 小腸における鉄吸収メカニズム (2010)
  • Author(s): 岸文雄
  • Journal Title: 日本医師会雑誌 Volume: 139 Pages: 291-294
• [Presentation] 網膜色素変性症を伴う脊髄後索型運動失調症(PCARP)におけるヘム輸送体FLVCR1の役割 (2012)
  • Author(s): 簗取いずみ、安井ゆみこ、三浦公志郎、岸文雄
  • Organizer: 第36回日本鉄バイオサイエンス学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-01
• [Presentation] Posterior column ataxia and retinitis pigmentosa (PCARP)で発見された変異型FLVCR1はヘム排出能力を喪失する (2012)
  • Author(s): 簗取いずみ
  • Organizer: 第53回日本生化学会中国・四国支部例会
  • Place of Presentation: 岡山大学 創立五十周年会館（岡山県）
• [Presentation] 網膜色素変性症を伴う脊髄後索型運動失調症(PCARP)におけるヘム輸送体FLVCR1の役割 (2012)
  • Author(s): 簗取いずみ
  • Organizer: 第36回日本鉄バイオサイエンス学会学術集会
  • Place of Presentation: 京王プラザホテル札幌（北海道）
• [Presentation] ヘム輸送体 Feline leukemia virus, subgroup C, receptor 1 (FLVCR1)の機能解析 (2011)
  • Author(s): 簗取いずみ,安井ゆみこ,三浦公志郎,河合泰宏,岸文雄
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-15
• [Presentation] ヘム輸送体Feline leukemia virus, subgroup C, receptor 1 (FLVCR1)の機能解析 (2011)
  • Author(s): 築取いずみ
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] 十二指腸における鉄吸収機構 (2011)
  • Author(s): 簗取いずみ、安井ゆみこ、三浦公志郎、河合泰宏、岸文雄
  • Organizer: 第35回日本鉄バイオサイエンス学会学術集会
  • Place of Presentation: 徳島市
  • Year and Date: 2011-09-11
• [Presentation] ヘムと非ヘム鉄の輸送寄港の解明 (2010)
  • Author(s): 簗取いずみ
  • Organizer: 第34回日本鉄バイオサイエンス学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-12