Expansion of activated NK cells from cord bloood

• Project/Area Number: 22591056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Hokkaido University
• Principal Investigator: TANAKA Junji 北海道大学, 大学院・医学研究科, 准教 (50250452)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: NK細胞 / 臍帯血 / 血液内科学 / 造血幹細胞移植 / 細胞療法

Research Abstract

We can expand NK cells more than 1,000 fold from cord blood after 3 weeks culture. These expanded NK cells can attack not only K562 leukemic cell line but also patients’ leukemic cells.

Research Products

• [Journal Article] Changes in incidence and caus es of non-relapse mortality after al logeneic hematopoietic cell transpla ntation in patients with acute leuke mia/myelodysplastic syndrome (2013)
  • Author(s): Kurosawa S, Tanaka J et al.
  • Journal Title: Bone M arrow Transplant Volume: 48(4) Pages: 529-536
  • Peer Reviewed
• [Journal Article] Allogeneic hematopoietic stem cell transplantation for intermedia te cytogenetic risk acute myeloid le ukemia in first complete remission. (2013)
  • Author(s): Imahashi N, Tanaka J et al.
  • Journal Title: Bone Marrow Transplant Volume: 48(1) Pages: 56-62
  • Peer Reviewed
• [Journal Article] Unrelated cord blood transplanta tion vs. related transplantation with HLA 1-antigen mismatch in the gr aft-versus-host direction. (2013)
  • Author(s): Kanda J, Tanaka J et al.
  • Journal Title: Leukemia Volume: 27(2) Pages: 286-294
  • Peer Reviewed
• [Journal Article] Dasati nib enhances the expansion of CD56+C D3- NK cells from cord blood (2012)
  • Author(s): Tanaka J et al.
  • Journal Title: Blood1 Volume: 19 Pages: 6175-6176
  • Peer Reviewed
• [Journal Article] Expan sion of NK cells from cord blood wit h antileukemic activity using GMP-co mpliant substances without feeder cells (2012)
  • Author(s): Tanaka J et al.
  • Journal Title: Leukemia Volume: 26 Pages: 1149-1152
  • Peer Reviewed
• [Journal Article] Related transplantation with HLA1-antigen mismatch in the graft-vers us-host direction and HLA 8/8-allele-matched unrelated transplantation:A nationwide retrospective study (2012)
  • Author(s): Kanda J, Tanaka J et al.
  • Journal Title: Blood Volume: 119 Pages: 2409-2416
  • Peer Reviewed
• [Journal Article] Efficacy of folinic acid in p reventing oral mucositis in allogene ic hematopoietic stem cell transplan t patients receiving MTX as prophylaxis for GVHD (2012)
  • Author(s): Sugita J, Tanaka J et al.
  • Journal Title: Bone Marrow Transpla nt Volume: 47 Pages: 258-264
  • Peer Reviewed
• [Journal Article] Expansion of NK cells from cord blood with antileukamic activity using GMP-compliant substances without feeder cells (2012)
  • Author(s): Tanaka J, et al.
  • Journal Title: Leukemia Volume: (in press) Issue: 5 Pages: 1149-1152
  • DOI: 10.1038/leu.2011.345
  • Peer Reviewed
• [Journal Article] Dasatinib enhances the expansion of CD56+CD3- NK cells from cord blood. (2012)
  • Author(s): Tanaka J
  • Journal Title: Blood Volume: 119 (25) Issue: 25 Pages: 6175-6176
  • DOI: 10.1182/blood-2012-03-416800
  • Peer Reviewed
• [Journal Article] Expansion of CD4+ CD25+ regul atory T cells from cord blood CD4+ cells-chain c ytokines (IL-2 and IL-15) and rapa mycin. (2011)
  • Author(s): Asanuma S, Tanaka J et al.
  • Journal Title: Ann Hematol Volume: 90 Pages: 617-624
  • Peer Reviewed
• [Journal Article] ZAPS is a potent stimulato r of signaling mediated by the RNA helicase RIG-I during antiviral r esponses (2011)
  • Author(s): Hayakawa S, Tanaka J et al.
  • Journal Title: Nature Immunol Volume: 12 Pages: 37-44
  • Peer Reviewed
• [Journal Article] p53 transactivation is inv olved in the antiproliferative act ivity of the putative tumor suppre ssor RBM5 (2011)
  • Author(s): Kobayashi T, Tanaka J et al.
  • Journal Title: Int J Cancer Volume: 128 Pages: 304-318
  • Peer Reviewed
• [Journal Article] Disease stage stratified ef fects of cell dose in unrelated bo ne marrow transplantation for hema tological malignancies: A report f rom Japan Marrow Donor Program. (2011)
  • Author(s): Inamoto Y, Tanaka J et al.
  • Journal Title: Bo ne Marrow Transaplant Volume: 46 Pages: 1192-1202
  • Peer Reviewed
• [Journal Article] The 8p11 myeloproliferativ e syndrome due to rare FGFR1OP2-FG FR1 fusion (2011)
  • Author(s): Onozawa M, Tanaka J et al.
  • Journal Title: Eur J Haematol Volume: 86 Pages: 347-349
  • Peer Reviewed
• [Journal Article] Expansion of CD4^+ CD25^+ regulatory T cells from cord blood CD4^+ cells using the common γ-chain cytokines (IL-2 and IL-15) and rapamycin. (2011)
  • Author(s): Asanuma S, Tanaka J, et al
  • Journal Title: Ann Hematol Volume: (In press)
  • NAID: 120004123455
  • Peer Reviewed
• [Journal Article] ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses (2011)
  • Author(s): Hayakawa S, Tanaka J, et al.
  • Journal Title: Nature Immunol Volume: 12 Pages: 37-44
  • NAID: 120003118156
  • Peer Reviewed
• [Journal Article] Clinical significance of minimal residual disease in adultacute lymphoblastic leukemia. (2010)
  • Author(s): Kikuchi M, Tanaka J, et al.
  • Journal Title: Inter J Hematol Volume: 92 Pages: 481-489
  • NAID: 10027673537
  • Peer Reviewed
• [Journal Article] Related transplantion with HLA 1-antigen mismatch in the graft-versus-host direction and HLA 8/8-allele-matched unrelated transplantation
  • Author(s): Kanda J, Tanaka J, et al.
  • Journal Title: Blood Volume: (in press)
  • Peer Reviewed
• [Presentation] 成人ALLに対する造血幹細胞移植 (2013)
  • Author(s): 田中淳司
  • Organizer: 第３５回 日本造血細胞移植学会総会
  • Place of Presentation: 石川県立音楽堂（石川県）
  • Invited
• [Presentation] 抗腫瘍効果におけるNK細胞の役割 (2012)
  • Author(s): 田中淳司
  • Organizer: 第78回青森血液疾患セミナー
  • Place of Presentation: 青森、ホテル青森(青森県)
  • Year and Date: 2012-09-19
• [Presentation] (2012)
  • Author(s): 田中淳司
  • Organizer: 血液がんの治療つばさ共催フォーラム
  • Place of Presentation: 札幌、北海道大学クラーク会館(北海道)
  • Year and Date: 2012-09-01
• [Presentation] 成人ALLの移植療法について (2012)
  • Author(s): 田中淳司
  • Organizer: 第7回TokaiLeukemiaClub
  • Place of Presentation: 名古屋、ミッドスクエア(愛知県)
  • Year and Date: 2012-03-03
• [Presentation] 抗腫瘍効果におけるNK細胞の役割 (2012)
  • Author(s): 田中淳司
  • Organizer: 函館血液疾患セミナー
  • Place of Presentation: 函館、ホテル函 館(北海道)
  • Year and Date: 2012-02-22
• [Presentation] Reduced-Intensity Versus Myeloablative Conditioning Allogeneic Hematopoietic Stem Cell Transplantation for Patients Aged Over 45 Years with AcuteLymphoblastic Leukemia (ALL) in Remission: A Study From the ALL Working Group of the Japan Society for Hematopoietic Cell Transplantation (JSHCT) (2012)
  • Author(s): Tanaka J, Kanamori H, Nishiwaki S, Ohashi K, Taniguchi S, Eto T, Nakamae H, Katayama Y, Morishima Y, Suzuki R, Kato K, Sakamaki H, Nishimoto N, Masauzi N
  • Organizer: 54th Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: Atlanta, Georgia WorldCongress Center (USA)
• [Presentation] Unfavorable Outcome of Single-Unit Umbilical Cord Blood Transplantation for Elderly Patients with Myelodysplastic Syndromes. (2012)
  • Author(s): Aoki K, Ishiyama K, Itonaga H, Fukuda T, Taniguchi S, Ueda Y, Doki N, Sugio Y, Morishima Y, Nagamura T, Tanaka J, Atsuta Y, Ishikawa T, Miyazaki Y.
  • Organizer: 54th Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: Atlanta, Georgia World Congress Center (USA)
• [Presentation] Role of Allogeneic Stem Cell Transplantation for Adult T-Cell Leukemia in an HTLV-1Non-Endemic Area of Japan. (2012)
  • Author(s): Shigematsu A, Tanaka J, Kobayashi N, Yasui H, Shindo M, Kakinoki Y, Iyama S, Kuroda H, Tsutsumi Y, Hashino S, Imamura M, Teshima T.
  • Organizer: 54th Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: Atlanta, Georgia World Congress Center (USA)
• [Presentation] Favorable Outcomes of Autologous Stem Cell Transplantation in Adult Patients with Philadelphia Chromosome-Negative Acute Lymphoblastic Leukemia in First Remission: On Behalf of the Adult Acute Lymphoblastic Leukemia Working Group of the Japan Society for Hematopoietic Cell Transplantation (JSHCT). (2012)
  • Author(s): Kato H, Kawase T, Kako S, Mizuta S, Ohashi K, Kurokawa M, Iwato K, Mori T, Suzuki R, Sakamaki H, Morishima Y, Tanaka J.
  • Organizer: 54th Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: Atlanta, Georgia World Congress Center (USA)
• [Presentation] Impact of ParentalDonor Type On Outcomes AfterHLA-Matched and HLA-MismatchedT-Cell-Replete Hematopoietic Cell Transplantation for Patients with Leukemia: A Retrospective Cohort Study. (2012)
  • Author(s): Ichinohe T, Kanda J, Inagaki J, Inoue M, Koh K, Kikuta A, Yabe H, Tanaka J, Atsuta Y, Kanda Y.
  • Organizer: 54th Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: Atlanta, GeorgiaWorld Congress Center (USA)
• [Presentation] The effect of dasatinib on the expansion of CD56+CD3- NK cells from cord blood (2012)
  • Author(s): Tanaka J, Sugita J, Shiratori S, Shigematsu A, Imamura M
  • Organizer: 41st Annual Meeting of InternationalSociety of Hematology (ISEH)
  • Place of Presentation: Amsterdam, Hotel Okura(Netherlands)
• [Presentation] Fludarabine-Based Reduced Intensity Hematopoietic Stem Cell Transplantation (RIST) for Patients Aged 50-70 Years with Acute Lymphoblastic Leukemia (ALL) in Remission: A Study From the ALL Working Group of the Japan Society for Hematopoietic Cell Transplantation (JSHCT). (2011)
  • Author(s): Kanamori H, Kako S, Kato H, Nishiwaki S, Furukawa T, Saito M, Taniguchi S, Nakamae H, Yujiri T, Naganuma-Inoue T, Morishima Y, Suzuki R, Sakamaki H, Tanaka J.
  • Organizer: 53rdAnnual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: San Diego, San Diego Convention Center(USA)
• [Presentation] Prognostic Factors of Reducing Relapse in Patients Undergoing Allogeneic Hematopoietic Cell Transplantation From Related Donor for Acute Myeloid Leukemia: The Japan Society for Hematopoietic Cell Transplantation (JSHCT) AML Working Group. (2011)
  • Author(s): Yano Y, Yokoyama H, Nishimura R, Fukuda T, Ogawa H, Ohashi K, Kanamori H, Tanaka J, Sakamaki H, Atsuta Y, Takami A.
  • Organizer: 53rd Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: San Diego, San Diego ConventionCenter (USA)
• [Presentation] Chemorefractory Disease At Transplantation, and Umbilical Cord Blood As Donor Source Were Adverse Prognostic Factors for Overall Survival After Allogeneic Stem Cell Transplantation for Follicular Lymphoma: Retrospective Study of the Japan Society of Hematopoietic Stem Cell Transplantation (JSHCT) Lymphoma Working Group (2011)
  • Author(s): Izutsu K, Suzuki R, Kako S, Sakai R, Mori T, Yano S, Kato J, Fukuda T, Taniguchi S, Takanashi M, Morishima Y, Tanaka J, Suzumiya J. Poor Performance Status
  • Organizer: 53rd Annual Meeting of American Society of Hematology (ASH)
  • Place of Presentation: San Diego, San DiegoConvention Center (USA)
• [Presentation] The comparative study for expansion of CD4+CD25+FoxP3+ regulatory T cells from cord blood CD4+ cells using IL-15 or IL-2 with rapamycin. (2011)
  • Author(s): Tanaka J, Sugita J, Asanuma S, Shiratori S, Shigematu1 A, Fujimoto K, Nishio M, Kondo T, Imamura M.
  • Organizer: 40th Annual Meeting of International Society of Hematology (ISEH)
  • Place of Presentation: Vancouver, Westin Bayshore Hotel (Canada)
• [Presentation] GVHD/GVL制御とNK細胞 (2011)
  • Author(s): 田中淳司
  • Organizer: 第33回日本造血細胞移植学会総会
  • Place of Presentation: 松山、愛媛県県民文化会館(愛媛県)
• [Presentation] The Yield of Peripheral Blood (PB) Graft and the Changes of Adhesion Molecules Expression on PB CD34Positive (CD34+) Cells before and during Administration of Granulocyte-colony stimulating factor (G-CSF) American Society for Blood and Marrow Transplantation (2011)
  • Author(s): Masauzi N, Tanaka J, Imamura M.
  • Place of Presentation: Hawaii, HawaiiConvention Center (USA)
• [Presentation] The comparative study for expansion of CD4+CD25+FoxP3+ regulatory T cells from cord blood CD4+ cells using IL-15 or IL-2 with rapamycin (2011)
  • Author(s): Tanaka J, et al.
  • Organizer: 40th International Society of Hematology
  • Place of Presentation: Vancouver, Canada
• [Presentation] 造血細胞移植とNK細胞 (2010)
  • Author(s): 田中淳司
  • Organizer: 第45回愛媛臨床血液懇話会
  • Place of Presentation: 松山、大和屋本店(愛媛県)
  • Year and Date: 2010-08-07
• [Presentation] 造血細胞移植とNK細胞 (2010)
  • Author(s): 田中淳司
  • Organizer: 第45回 愛媛臨床血液懇話会
  • Place of Presentation: 松山 大和屋本店 招待講演
  • Year and Date: 2010-08-07
• [Presentation] Retrospective analysis for 272 patients with mature T-cell neoplasmas in Hokkaido, Japan (2010)
  • Author(s): Shigematsu A, Kobayashi N, Minauchi K, Kobayashi H, Mori A, Masauzi N, Umehara S, Fukuhara T, Kurosawa M, Haseyama M, Hashino S, Nishio M, Kondo T, Tanaka J, Kasai M, Imamura M
  • Organizer: 15st Congres of European Hematology Association
  • Place of Presentation: Barcelona, Barcelona Convention Center (Spain)
• [Presentation] 抗腫瘍効果におけるNK細胞の役割
  • Author(s): 田中淳司
  • Organizer: 第７８回青森血液疾患セミナー
  • Place of Presentation: ホテル青森（青森県）
  • Invited
• [Book] Annual Review 血液2012、造血幹細胞移植におけるGVHD/GVL制御とNK細胞 (2012)
  • Author(s): 田中淳司
  • Publisher: 中外医学社
• [Patent(Industrial Property Rights)] NK細胞を増幅するための組成物及び方法 (2011)
  • Inventor(s): 田中淳司
  • Industrial Property Rights Holder: テラ株式会社
  • Industrial Property Number: 2011-140504
  • Filing Date: 2011-06-24