| Project/Area Number |
22591061
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHITANI Akiko 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
MIURA Masako 京都大学, 医学研究科, 特定講師 (80437235)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 間葉系幹細胞 / HLAクラスIb分子 / 免疫制御 / 造血幹細胞移植 / 再生医療 / 移植・再生医療 / HLA-G / HLA-F |
|---|
| Research Abstract |
We have investigated the expression of HLA class Ib molecules in human bone marrow-derived mesenchymal stromal/stem cells (BM-MSCs) at mRNA and protein levels. HLA-E and HLA-F mRNA transcripts were detected in quiescent BM-MSCs but HLA-G mRNA and its alternatively spliced forms were hardly detectable. Treatment of BM-MSCs by IL-10 did not induce mRNA expression of HLA-G. At the protein level, although BM-MSCs were reported to express classical HLA class Ia molecules on their cell surface, we could not detect surface expression of HLA-F and HLA-G. Future research is warranted to evaluate the expression of HLA class Ib molecules in MSCs or MSC-like cells derived from tissues other than bone marrow.
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