Functional expression of HLA class Ib molecules in bone marrow-derived mesenchymal stromal cells and its implication for cellular immunotherapy
| Project/Area Number |
22591061
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHITANI Akiko 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
MIURA Masako 京都大学, 医学研究科, 特定講師 (80437235)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 間葉系幹細胞 / HLAクラスIb分子 / 免疫制御 / 造血幹細胞移植 / 再生医療 / 移植・再生医療 / HLA-G / HLA-F |
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| Research Abstract |
We have investigated the expression of HLA class Ib molecules in human bone marrow-derived mesenchymal stromal/stem cells (BM-MSCs) at mRNA and protein levels. HLA-E and HLA-F mRNA transcripts were detected in quiescent BM-MSCs but HLA-G mRNA and its alternatively spliced forms were hardly detectable. Treatment of BM-MSCs by IL-10 did not induce mRNA expression of HLA-G. At the protein level, although BM-MSCs were reported to express classical HLA class Ia molecules on their cell surface, we could not detect surface expression of HLA-F and HLA-G. Future research is warranted to evaluate the expression of HLA class Ib molecules in MSCs or MSC-like cells derived from tissues other than bone marrow.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Donor/Source Working Group and HLA Working Group of the Japan Society for Hematopoietic Cell Transplantation. Unrelated cord blood transplantation vs. related transplantation with HLA 1-antigen mismatch in the graft-versus-host direction2013
Author(s)
Kanda J, Ichinohe T, Kato S, Uchida N,Terakura S, Fukuda T, Hidaka M, Ueda Y, Kondo T, Taniguchi S, Takahashi S, Nagamura-Inoue T, Tanaka J, Atsuta Y,Miyamura K, Kanda K
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Journal Title
Leukemia
Volume: 27
Pages: 286-294
Related Report
Peer Reviewed
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[Journal Article] HLA Working Group of the Japan Society for Hematopoietic Cell Transplantation. Impact of the direction of HLA mismatch on transplantation outcomes in single unrelated cord blood transplantation2013
Author(s)
Kanda J, Atsuta Y, Wake A, Ichinohe T,Takanashi M, Morishima Y, Taniguchi S, Takahashi S, Ogawa H, Ohashi K, Ohno Y, Aotsuka N, Onishi Y, Kato K, Nagamura-Inoue T, Kanda Y
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Journal Title
Biol Blood Marrow Transplan
Volume: 19
Pages: 247-254
Related Report
Peer Reviewed
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