| Project/Area Number |
22591062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
ORITANI Kenji 大阪大学, 医学系研究科, 准教授 (70324762)
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| Co-Investigator(Kenkyū-buntansha) |
KANAKURA Yuzuru 大阪大学, 医学系研究科, 教授 (20177489)
EZOE Sachiko 大阪大学, 医学部附属病院, 特任講師(常勤) (90379173)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 免疫 / 炎症 / リンパ球 / 分化 / 増殖 / 遺伝子改変マウス / マクロファージ / OSF-5 / STAP-2 / 腸炎モデル / キメラマウス / サイトカイン / OSF-5(Pleiotrophin) |
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| Research Abstract |
We analyzed in vivo effects of signal-transducing adaptor protein-2 (STAP-2) as well as proliferin, pleiotrophin, and osteoblast stimulating factor (OSF)-5. We established knock-in chimera mice to produce proliferin, pleiotrophin, or OSF-5, respectively. Only OSF-5-KI chimera mice showed lymphocytopenia in peripheral blood, spleen, and bone marrow. The mice had few B lymphocytes after pre-B cell differentiation. In addition, OSF-5 was produced by stromal cells. We also evaluated DSS-induced colitis in signal-transducing adaptor protein-2 (STAP-2)-knockout mice. STAP-2 deficiency resulted in less body weight loss and colon shortening. Colon cells had responsibility to less phenotypes of colitis, and production of chemokines was impaired in STAP-2-knockout mice.
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