| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Research Abstract |
Systemic lupus erythematosus (SLE) is on of the systemic autoimmune diseases that is characterized by production of various autoantibodies including anti-double strand (ds)-DNA antibodies from B cells. In the pathogenesis of SLE, autoantibody-producing B cells may play a pivotal role in developing autoimmunity. Therefore, understanding of human autoantibody-producing B cell is an essential issue. We investigated phenotype of RP105-negative B cell subsets that produce anti-dsDNA antibodies and are increased in patients with SLE. Phenotyping RP105-negaive B cell subsets was performed using a flow cytometer. RP105-negative B cells consist of at least 5 subsets of late B cells, including CD19(+)RP105(int), CD19(+)RP105(-), CD19(low)RP105(-)CD138(-), CD19(low)RP105(-)CD138(int), and CD19(low)RP105(-)CD138(++) B cells. CD19(+)RP105(int) and CD19(low)RP105(-)CD138(int) B cells are larger population than other RP105(-) B cell subsets in SLE. The phenotypic analysis of RP105(-) B cell subsets suggests dysregulation of later B cell subsets in SLE and may provide new insights into understanding regulation of late B cells in human.
|
