Color-codedreal-time cellular imaging of lung T-lymphocyte ina mouse asthma model

• Project/Area Number: 22591093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Yamaguchi University
• Principal Investigator: HASEGAWA Akihiro 山口大学, 大学院・医学系研究科, 大学院医学系研究科 (80376376)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Toshinori 千葉大学, 大学院・医学研究院, 教授 (50237468)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アレルギー / 喘息 / 炎症 / 細胞イメージング / イメージング / T細胞 / ぜんそく

Research Abstract

We developed a color-coded real-time imaging model of cell migration into the lung in a mouse model of asthma, and identify the cellular process required for the initiation of the asthma phenotype. Selective accumulation of antigen-specific CD4 T cells in the lung was quantitatively imaged. Accumulating Th2 cells, but not Th1 cells, formed foci in the lung after antigen inhalation.

Research Products

• [Journal Article] Crucial role for CD69 in the pathogenesis of dextran sulphate sodium-induced colitis (2013)
  • Author(s): Hasegawa, A.,Iwamura, C., Kitajima, M., Hashimoto, K., Otsuyama, K., Ogino, H., Nakayama, T. and Shirai, M
  • Journal Title: PLoS ONE Volume: (in press)
  • Peer Reviewed
• [Journal Article] Functionally distinct Gata3/Chd4 complexes coordinately establish T helper 2 (Th2) cell identity. (2013)
  • Author(s): Hosokawa H
  • Journal Title: Proc Natl Acad Sci U SA Volume: - Issue: 12 Pages: 4691-4696
  • DOI: 10.1073/pnas.1220865110
  • Peer Reviewed
• [Journal Article] Crucial role for CD69 in the pathogenesis of dextran sulphate sodium-induced colitis. (2013)
  • Author(s): Hasegawa, A.
  • Journal Title: PLoS ONE Volume: in press
  • Peer Reviewed
• [Journal Article] Type II membrane protein CD69 regulates the formation of resting T-helper memory (2012)
  • Author(s): Shinoda, K., Tokoyoda, K., Hanazawa, A., Hayashizaki, K., Zehentmeier, S., Hosokawa, H., Iwamura, C, Koseki, H., Tumes, D.J., Radbruch, A., Nakayama T.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: (in press) Issue: 19 Pages: 7409-7414
  • DOI: 10.1073/pnas.1118539109
  • Peer Reviewed
• [Journal Article] The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-・ and suppresses TH2 differentiation (2012)
  • Author(s): Kuwahara, M., Yamashita, M., Shinoda, K., Tofukuji, S., Onodera, A., Shinnakasu, R., Motohashi, S., Hosokawa, H., Tumes, D., Iwamura, C., Lefebvre, V., and Nakayama, T.
  • Journal Title: Nat. Immunol Volume: 13(8) Issue: 8 Pages: 778-786
  • DOI: 10.1038/ni.2362
  • Peer Reviewed
• [Journal Article] Regulation of memory CD4 T-cell pool size and function by natural killer T cells in vivo. 109(42) : 16992-16997 (2012). (2012)
  • Author(s): Iwamura, C., Shinoda, K., Endo, Y., Watanabe, Y., Tumes, J. D., Motohashi, S., Kawahara, K., Kinjo, Y., and Nakayama, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Issue: 42 Pages: 16992-16997
  • DOI: 10.1073/pnas.1203494109
  • Peer Reviewed
• [Journal Article] 喘息肺での浸潤リンパ球のin vivoイメージング (2012)
  • Author(s): 長谷川 明洋
  • Journal Title: 日本臨床免疫学会会誌 Volume: 35 Pages: 319-319
• [Journal Article] Apolipoprotein A-II suppressed concanavalin A-induced hepatitis via the inhibition of CD4 T cell function (2011)
  • Author(s): Yamashita, J., Iwamura, C., Sasaki, T., Mitsumori, K., Ohshima, K., Hada, K., Hara, N., Takahashi, M., Kaneshiro, Y., Tanaka, H., Kaneko, K. Nakayama, T
  • Journal Title: J. Immunol Volume: 186 Issue: 6 Pages: 3410-3420
  • DOI: 10.4049/jimmunol.1002924
  • Peer Reviewed
• [Journal Article] Comparion of cancer cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse (2011)
  • Author(s): Hayashi, K., Kimura, H., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Hasegawa, A.,Bouvet, M and Hoffman, R.M
  • Journal Title: Anticancer Res Volume: 31 Pages: 3665-3672
  • Peer Reviewed
• [Journal Article] Complete genome sequence of NBRC 3288, a unique cellulose-nonproducing strain of Gluconacetobacter xylinus isolated from vinegar (2011)
  • Author(s): Ogino H, et al
  • Journal Title: J Bacteriol Volume: 193(24) Issue: 24 Pages: 6997-6998
  • DOI: 10.1128/jb.06158-11
  • Peer Reviewed
• [Journal Article] Genome-wide analysis reveals unique regulation of transcription of Th2-specific genes by GATA3 (2011)
  • Author(s): Horiuchi, S., Onodera, A., Hosokawa, H., Watanabe, Y., Tanaka, T., Sugano, S., SuzukiY. Nakayama, T
  • Journal Title: J. Immunol Volume: 186 Issue: 11 Pages: 6378-6389
  • DOI: 10.4049/jimmunol.1100179
  • Peer Reviewed
• [Journal Article] Memory type 2 helper T cells induce long-lasting anti-tumor immunityby activating natural killer cells (2011)
  • Author(s): Kitajima, M., Ito, T., Tumes, J. D., Endo, Y., Onodera, A., Hashimoto, K., Motohashi, S., Yamashita, M., Nishimura, T., Ziegler, F. S. Nakayama, T
  • Journal Title: Cancer Res Volume: 71 Issue: 14 Pages: 4790-4798
  • DOI: 10.1158/0008-5472.can-10-1572
  • Peer Reviewed
• [Journal Article] Eomesodermin controls interleukin-5 production in memory T helper 2 cells through inhibition of activity of the transcription factor GATA3 (2011)
  • Author(s): Endo, Y., Iwamura, C., Kuwahara, M., Suzuki, A., Sugaya, K., Tumes, J. D., Tokoyoda, K. Hosokawa, H., Yamashita, M. Nakayama, T
  • Journal Title: Immunity Volume: 35 Issue: 5 Pages: 733-745
  • DOI: 10.1016/j.immuni.2011.08.017
  • Peer Reviewed
• [Journal Article] Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the l ive mouse (2011)
  • Author(s): Hayashi, K.
  • Journal Title: Anticancer Research Volume: 31 Pages: 3665-3672
  • Peer Reviewed
• [Journal Article] Role of CD69 in the pathogenesis of inflammation (2010)
  • Author(s): Hasegawa, A. and Nakayama, T
  • Journal Title: Jpn. J. Clin. Immunol. Volume: 33(4) Pages: 189-195
  • NAID: 10027753650
• [Journal Article] lColor-coded real-time cellular imaging of T lymphocyte accumulation and focus formation in mouse asthma model (2010)
  • Author(s): Hasegawa, A., Hayashi, K., Kishimoto, H., Yang, M., Tofukuji, S., Suzuki, K., Nakajima, H., Hoffman, R. M., Shirai, M. Nakayama, T
  • Journal Title: J Allergy Clin. Immunol Volume: 125 Issue: 2 Pages: 461-468
  • DOI: 10.1016/j.jaci.2009.09.016
  • Peer Reviewed
• [Journal Article] Color-coded real-time cellular imaging of T-lymphocyte accumulation and focus formation in a mouse asthma model. (2010)
  • Author(s): Hasegawa, A.
  • Journal Title: J.Allergy and Clin.Immunol. Volume: VOL.125 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Role of CD69 in the pathogenesis of inflammation. (2010)
  • Author(s): Hasegawa, A.
  • Journal Title: Jpn.J.Clin.Immunol. Volume: VOL.33 Pages: 189-195
  • NAID: 10027753650
• [Journal Article] 喘息肺でのTh2細胞リアルタイムin vivoイメージング (2010)
  • Author(s): 長谷川明洋
  • Journal Title: 医学のあゆみ Volume: 232巻 Pages: 1259-1265
• [Presentation] Real-time cellular imaging of T lymphocyte accumulation in a mouse asthma model (2013)
  • Author(s): Hasegawa, A., Ogino, H., Shirai, M. and Nakayama, T
  • Organizer: 23th CDB Meeting
  • Place of Presentation: RIKEN Center for Developmental Biology (Kobe)
• [Presentation] 腸炎の発症における CD69 分子の役割 (2013)
  • Author(s): 長谷川明洋、荻野英賢、大津山賢一郎、中山俊憲、白井睦訓
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ(千葉)
• [Presentation] 10 種類の肺炎原因菌を検出する Multiplex NASBA-核酸クロマト検出法 (2013)
  • Author(s): 知識麻友子、林将大、宇治家武史、荻野英賢、大津山賢一郎、長谷川明洋、宮下修行、江崎孝行、白井睦訓
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ(千葉)
• [Presentation] 腸炎の発症におけるCD69分子の役割 (2013)
  • Author(s): 長谷川 明洋
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] 10種類の肺炎原因菌を検出するMultiplex NASBA-核酸クロマト検出法 (2013)
  • Author(s): 知識 麻友子
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] 免疫記憶を利用した難治性喘息と肺癌の新規免疫治療法開発のトランスレ-ショナルリサ-チ 特別講演 BIO tech 2012 (2012)
  • Author(s): 中山俊憲
  • Organizer: 第11回国際バイオテクノロジ-展/技術会議
  • Place of Presentation: 東京ビッグサイト(東京)
  • Year and Date: 2012-04-25
• [Presentation] 喘息肺での浸潤リンパ球のリアルタイムイメージング (2012)
  • Author(s): 長谷川明洋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎新聞文化ホール(長崎市)(招待講演)
  • Year and Date: 2012-03-28
• [Presentation] 酢酸菌高温耐性株における細胞形態形成機構の解析 (2012)
  • Author(s): 荻野英賢、長谷川明洋、大津山賢一郎、白井睦訓
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島大学(徳島)
• [Presentation] 喘息肺での浸潤リンパ球のin vivoイメージング (2012)
  • Author(s): 長谷川明洋、白井睦訓、中山俊憲
  • Organizer: 第40回日本臨床免疫学会総会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] アレルギー性炎症反応におけるリンパ球浸潤のin vivoイメージング (2012)
  • Author(s): 長谷川明洋、荻野英賢、大津山賢一郎、白井睦訓、中山俊憲
  • Organizer: 第21回日本バイオイメージング学会学術集会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] 喘息肺での浸潤リンパ球のリアルタイムイメージング (2012)
  • Author(s): 長谷川明洋、白井睦訓、中山俊憲
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)
• [Presentation] アレルギー性炎症反応におけるリンパ球浸潤のin vivoイメージング (2012)
  • Author(s): 長谷川 明洋
  • Organizer: 第21回日本バイオイメージング学会学術集会
  • Place of Presentation: 国立京都国際会館（京都市）
• [Presentation] 喘息肺での浸潤リンパ球のin vivoイメージング (2012)
  • Author(s): 長谷川 明洋
  • Organizer: 第40回日本臨床免疫学会総会
  • Place of Presentation: 京王プラザホテル（東京都）
  • Invited
• [Presentation] 酢酸菌高温耐性株における細胞形態形成機構の解析 (2012)
  • Author(s): 荻野 英賢
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島大学（徳島市）
• [Presentation] Crucial role for CD69 in the pathogenesis of DSS-induced colitis (2011)
  • Author(s): Akihiro Hasegawa
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2011-11-28
• [Presentation] NKT cell-mediated regulation of antigen-specific memory Th2 cell function (2011)
  • Author(s): Nakayama, T., and Iwamura, C
  • Organizer: 6th International Symposium on CD1 and NKT Cells
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-09-25
• [Presentation] 炎症の誘導にともなうリンパ球浸潤のバイオイメージング (2011)
  • Author(s): 長谷川明洋
  • Organizer: 第20回日本バイオイメージング学会学術集会
  • Place of Presentation: 千歳科学技術大学(千歳市)(招待講演)
  • Year and Date: 2011-09-02
• [Presentation] Epigenetic regulation of GATA3 expression and its target genes in Th2 cells (2011)
  • Author(s): Nakayama, T
  • Organizer: 45thJoint Working Conference on Immunology and Viral Diseases
  • Place of Presentation: CA, USA
  • Year and Date: 2011-06-20
• [Presentation] Regulation of memory Th2 cell function and allergic airway inflammation via polycomb and trithorax molecules (2011)
  • Author(s): Nakayama, T., and Onodera, A
  • Organizer: 30th Congress of the European Academy of Allergy and Clinical Immunology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-06-15
• [Presentation] Epigenetic control of memory Th2 cell function via Polycomb and Trithorax molecules (2011)
  • Author(s): Nakayama, T., and Onodera, A
  • Organizer: EUThyme-Rolduc Meeting
  • Place of Presentation: Leeuwenhorst, Netherland
  • Year and Date: 2011-05-24
• [Presentation] Crucial role for CD69 in the pathogenesis of DSS-induced colitis. (2011)
  • Author(s): Hasegawa, A. and Nakayama, T
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
• [Presentation] 炎症の誘導にともなうリンパ球浸潤のバイオイメージング (2011)
  • Author(s): 長谷川明洋
  • Organizer: 第20回日本バイオイメージング学会学術集会シンポジウム
  • Place of Presentation: 千歳科学技術大学(北海道)
• [Presentation] Real-time cellular imaging of T lymphocyte accumulation in a mouse asthma model. (2010)
  • Author(s): Hasegawa, A.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-08-26
• [Presentation] 喘息肺での浸潤リンパ球のリアルタイムイメージング (2010)
  • Author(s): 長谷川明洋、中山俊憲、白井睦訓
  • Organizer: 第19回日本バイオイメージング学会学術集会
  • Place of Presentation: 慶應義塾大学(東京)
• [Presentation] Real-time cellular imaging of T lymphocyte accumulation in a mouse asthma model (2010)
  • Author(s): Hasegawa, A., Shirai, M. and Nakayama, T
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe International Exhibition Hall (Kobe).
• [Presentation] 喘息肺での浸潤リンパ球のリアルタイムイメージング (2010)
  • Author(s): 長谷川明洋
  • Organizer: 第19回日本バイオイメージング学会学術集会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Remarks] ホームページ
  • URL: http://www.med.yamaguchi-u.ac.jp/medicine/chair/basic_09.html
• [Remarks] 山口大学大学院医学系研究科 ゲノム・機能分子解析学分野
  • URL: http://mb.med.yamaguchi-u.ac.jp/
• [Remarks]
  • URL: http://www.med.yamaguchi-u.ac.jp/
• [Remarks]
  • URL: http://mb.med.yamaguchi-u.ac.jp/
• [Patent(Industrial Property Rights)] CD69アンタゴニストを含む肝炎治療用組成物 (2011)
  • Inventor(s): 中山俊憲、長谷川明洋、白井睦訓
  • Industrial Property Rights Holder: 国立大学法人千葉大学、国立大学法人山口大学
  • Industrial Property Number: 2011-247036
  • Filing Date: 2011-11-11
• [Patent(Industrial Property Rights)] Imageable rodent model of asthma (2011)
  • Inventor(s): Akihiro Hasegawa, Toshinori Nakayama, Meng Yang
  • Industrial Property Rights Holder: AntiCancer Inc
  • Acquisition Date: 2011-02-10
  • Overseas
• [Patent(Industrial Property Rights)] INHIBITION OF CD69 FOR TREATMENT OF INFLAMMATORY CONDITIONS (2010)
  • Inventor(s): Toshinori Nakayama, Akihiro Hasegawa, Mutusnori Shirai
  • Industrial Property Rights Holder: 国立大学法人千葉大学、国立大学法人山口大学
  • Filing Date: 2010-11-12
  • Overseas
• [Patent(Industrial Property Rights)] Inhibition of CD69 for treatment of inflammatory conditions (2010)
  • Inventor(s): 中山俊憲、長谷川明洋、白井睦訓
  • Industrial Property Rights Holder: 千葉大学、山口大学
  • Filing Date: 2010-11-12
  • Overseas