A mechanism of platelet phagocytosis in dengue virus infection

• Project/Area Number: 22591106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: National Institute of Infectious Diseases (2012); Osaka University (2010-2011)
• Principal Investigator: OISHI Kazunori 国立感染症研究所, 感染症情報センター, センター長 (80160414)
• Co-Investigator(Kenkyū-buntansha): KUROSU Takeshi 大阪大学, 微生物病研究所, 助教 (70432432)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: デングウイルス / 血小板減少 / 血小板アポトーシス / 血小板 / 巨核球 / 骨髄系細胞 / インターフェロンレセプター欠損マウス / デング / 二次感染 / アポトーシス / アポトーシス血小板クリアランス / 血小板減少症 / フィリピン

Research Abstract

Dengue illness has become a major public health concern particularly in tropical countries and the mechanism of thrombocytopenia remains uncertain. We previously reported from the clinical study in the Philippines a significant association between thrombocytopenia and an increased phagocytosis of platelets in an ex vivo setting during acute phase of secondary dengue virus infection (Honda S, et al. Am J Trop Med Hyg, 2009). We report here an increased phagocytosis of DV-induced apoptotic platelets by macrophages via a phosphatidylserine-recognizing pathway in secondary DV infection during the acute phase. Accelerated platelet clearance was overcome by thrombopietin-induced enhanced thrombopoiesis in these patients (Alonzo T, et al. J infect Dis, 2012).

Research Products

• [Journal Article] Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection. (2013)
  • Author(s): Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leaungwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K
  • Journal Title: Antiviral Res Volume: 98(3) Pages: 423-31
• [Journal Article] Platelet apoptosis and apoptotic platelet clearance by macrophages in secondary dengue virus infections. (2012)
  • Author(s): Alonzo MTG, Lacuesta TLV, Dimaano EM, Kurosu T, Suarez LC, Mapua CA, Akeda Y, Matias RR, Kuter DJ, Nagata S, Natividad FF, Oishi K
  • Journal Title: J Infect Dis Volume: 205(8) Pages: 1321-9
• [Journal Article] Limited cross-reactivity of mouse monoclonal antibodies against Dengue virus capsid protein among four serotypes. (2012)
  • Author(s): Noda M, Masrinoul P, Punkum C, Pipattanaboon C, Ramasoota P, Setthapramote C, Sasaki T, Sasayama M, Yamashita A, Kurosu T, Ikuta K, Okabayashi T
  • Journal Title: Biologics Volume: 6 Pages: 409-16
• [Journal Article] Limited cross-reactivity of mouse monoclonal antibodies against dengue virus capsid protein among four serotypes (2012)
  • Author(s): Noda M
  • Journal Title: Biologics Volume: 6 Pages: 409-416
  • Peer Reviewed
• [Journal Article] Platelet Apoptosis and Apoptotic Platelet Clearance by Macrophages in Secondary Dengue Virus Infections. (2012)
  • Author(s): Alonzo, M., Lacuesta, T., Dimaano, EM., Kurosu, T., Suarez, LC., Mapua, CA Akeda, Y., Matias, RR., Kuter, DJ., Nagata, S., Natividad, FF. and Oishi, K.
  • Journal Title: J Infect Dis. Volume: 205 Issue: 8 Pages: 1321-1329
  • DOI: 10.1093/infdis/jis180
  • Peer Reviewed
• [Presentation] 感染症の将来を考える:臨床と基礎の融合を目指して (2012)
  • Author(s): 大石和徳
  • Organizer: 日本感染症学会東日本地方会学術集会
• [Presentation] 感染症の将来を考える：臨床と基礎の融合を目指して (2012)
  • Author(s): 大石和徳
  • Organizer: 第61回日本感染症学会東日本地方会学術集会
  • Place of Presentation: 東京
• [Presentation] 治療法開発のためのデングウイルス感染マウスモデル (2012)
  • Author(s): 黒須 剛
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
• [Presentation] Role of apoptotic platelet clearance in thrombocytopenia in dengue, a reemerging infectious disease. (2011)
  • Author(s): Oishi K
  • Organizer: Symposium, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] A role of apoptosis and apoptotic platelet clearance in secondary dengue virus infection. (2011)
  • Author(s): Oishi K, Alonzo MTG
  • Organizer: IDSA 49 th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] A role of platelet apoptosis and apoptotic platelet clearance othrombocytopenia in secondary dengue vcirus infection (2011)
  • Author(s): Oishi K, et al
  • Organizer: platelet clearanceon IDSA 49 th annual meeting
  • Place of Presentation: ボストン、米国
• [Presentation] Accelerated platelet apoptosis is associated with platelet phagocytosis and thrombocytopenia in secondary dengue virus infection (2010)
  • Author(s): Maria T Alonzo, et al.
  • Organizer: Am Soc Trop Med Hyg 59th Annual Meeting
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2010-11-07
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/lab/CRID/index.html
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/lab/CRID/index.html