Development of Early Detection kit for diagnosis mucormycosis

• Project/Area Number: 22591110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Nagasaki University
• Principal Investigator: KAKEYA Hirosi 長崎大学, 医歯薬学総合研究科, 准教授 (40398152)
• Co-Investigator(Kenkyū-buntansha): KOUNO Sigeru 長崎大学, 医歯薬学総合研究科, 教授 (80136647)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 接着菌 / シグナルシークエンス / 診断キット / 接合菌

Research Abstract

The diagnosis of mucormycosis relies upon the identification of organisms in tissue by histopathology with culture confirmation. However, culture often yields no growth, and histopathologic identification of an organism with a structure typical of Mucorales may provide the only evidence of infection. A clinician must think of this entity in the appropriate clinical setting and pursue invasive testing in order to establish a diagnosis as early as possible. Therefore, new early detection methods are required. We used a novel signal sequence trap methods to select protein antigen from Rhizopus oryzae. Two unknown proteins with signal sequences, antigen A (26kDa, hypothetical protein) and B (46kDa, hypothetical protein) were selected as candidates. We made ELISA kit to detect antigen A. The antigen A was detected in the culture supernatant of R. oryzae、R microspores、R microspores var. rhizopodiformis. The antigen was also detected in the serum form Rhizopus infected mice.

Research Products

• [Journal Article] Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan (2013)
  • Author(s): Mihara T, Izumikawa K, Kakeya H, Ngamskulrungroj P, Umeyama T, Takazono T, Tashiro M, Nakamura S, Imamura Y, Miyazaki T, Ohno H, Yamamoto Y, Yanagihara K, Miyzaki Y, Kohno S
  • Journal Title: Med Mycol Volume: 51 Pages: 252-256
• [Journal Article] Clinical efficacy and safety micafungin in Japanese patients with chronic pulmonary aspergillosis: a prospective observational study (2012)
  • Author(s): Kohno S, Izumikawa K, Kakeya H, Miyazaki Y, Ogawa K, Amitani R, Niki Y, Kurashima A
  • Journal Title: Med Mycol. Volume: 49 Pages: 688-693
  • NAID: 120006985289
• [Journal Article] Antifungal susceptibilities of Aspergillus fumigatus clinical isolates in Nagasaki, Japan (2012)
  • Author(s): Tashiro M, Izumikawa K, Minematsu A, Hirano K, Iwanaga N, Ide S, Mihara T, Hosogaya N, Takazono T, Morinaga Y, Nakamura S, Kurihara S, Imamura Y, Miyazaki T, Nishino T, Tsukamoto M, Kakeya H, Yamamoto Y, Yanagihara K, Yasuoka A, Tashiro T, Kohno S.
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Pages: 584-587
• [Journal Article] Bronchoalveolar lavage galactomannan for the diagnosis of chronic pulmonary aspergillosis (2012)
  • Author(s): Izumikawa K, Yamamoto Y, Mihara T, Takazono T, Morinaga Y, Kurihara S, Nakamura S, Imamura Y, Miyazaki T, Nishino T, Tsukamoto M, Kakeya H, Yanagihara K, Mine M, Yasuoka A, Tashiro T, Kohno S
  • Journal Title: Med Mycol Volume: 50 Pages: 811-817
  • NAID: 120006985848
• [Journal Article] Correlation between triazole treatment history and susceptibility in clinically isolated Aspergillus fumigatuas (2012)
  • Author(s): Tashiro M, Izumikawa K, Hirano K, Ide S, Mihara T, Hosogaya N, Takazono T, Morinaga Y, Nakamura S, Kurihara S, Imamura Y, Miyazaki T, Nishino T, Tsukamoto M, Kakeya H, Ya ma mo t o Y, Yanagihara K, Yasuoka A, Tashiro T, Kohno S
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Pages: 4870-4875
• [Journal Article] The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata (2011)
  • Author(s): Miyazaki T, Izumikawa K, Yamauchi S, Inamine T, Nagayoshi Y, Saijo T, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Miyazaki Y, Yasuoka A, Kohno S
  • Journal Title: FEMS Yeast Res Volume: 11 Pages: 449-456
  • NAID: 120006984996
• [Journal Article] Evaluation of the Cica Fungi Te st Candida, a novel serum Candida mannan antigen kit, and its comparison with Cand-Tec in candidemia patients (2011)
  • Author(s): Takazono T, Izumikawa K, Nagayoshi Y, Tanaka A, Mihara T, Kosai K, Saijo T, Imamura Y, Miyazaki T, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Kamihira S, Kohno S
  • Journal Title: Jpn J Infect Dis Volume: 64 Pages: 116-120
  • NAID: 120006985095
• [Journal Article] Synergistic antifungal effect of lactoferrin with azole antifungals against Candida albicans and a proposal for a new treatment method for invasive candidiasis (2011)
  • Author(s): Kobayashi T, Kakeya H, Miyazaki T, Izumikawa K, Yanagihara K, Ohno H, Yamamoto Y, Tashiro T, Kohno S
  • Journal Title: Jpn J Infect Dis Volume: 64 Pages: 292-296
  • NAID: 40018924977
• [Journal Article] Functional characterization of the regulators of calcineurin in Candida glabrata (2011)
  • Author(s): Miyazaki T, Izumikawa K, Nagayoshi Y, Saijo T, Yamauchi S, Morinaga Y, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Miyazaki Y, Kohno S
  • Journal Title: FEMS Yeast Res Volume: 11 Pages: 621-630
  • NAID: 120006985354
• [Journal Article] Diagnostic significance of Aspergillus species isolated from respiratory samples in an adult pneumology ward (2011)
  • Author(s): Tashiro T, Izumikawa K, Tashiro M, Takazono T, Morinaga Y, Yamamoto K, Imamura Y, Miyazaki T, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Yasuoka A, Kohno S
  • Journal Title: Med Mycol Volume: 49 Pages: 581-585
  • NAID: 120006985275
• [Journal Article] Skn7p is required for oxidative stress response and virulence in Candida glabrata (2010)
  • Author(s): Saijo T, Miyazaki T, Izumikawa K, Mihara T, Takazono T, Kosai K, Imamura Y, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Kohno S
  • Journal Title: Mycopathologia Volume: 169 Pages: 81-90
• [Journal Article] Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of Candida glabrata (2010)
  • Author(s): Miyazaki T, Inamine T, Yamauchi S, Nagayoshi Y, Saijo T, Izumikawa K, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Miyazaki Y, Kohno S
  • Journal Title: Antimicrob Agents Chemother Volume: 54 Pages: 1639-1643
  • NAID: 120006984565
• [Journal Article] Intravenous micafungin versus voriconazole for chronic pulmonary aspergillosis: a multicenter trial in Japan (2010)
  • Author(s): Kohno S, Izumikawa K, Ogawa K, Kurashima A, Okimoto N, Amitani R, Kakeya H, Niki Y, Miyazaki Y
  • Journal Title: J Infecion Volume: 61 Pages: 410-418
  • NAID: 120006984951
• [Presentation] 新規蛋白抗原を使用した接合菌症の早期診断法開発の試み (2013)
  • Author(s): 掛屋 弘、他
  • Organizer: 第14回真菌症フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2013-02-16
• [Presentation] 新規蛋白抗原を使用した接合菌症の早期診断法開発の試み
  • Author(s): 掛屋 弘
  • Organizer: 第14回真菌症フォーラム
  • Place of Presentation: 東京