| Project/Area Number |
22591119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAJIMA Toshihiro 北海道大学, 医学研究科, 講師 (50333597)
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| Co-Investigator(Renkei-kenkyūsha) |
OHMIYA Yoshihiro 独立行政法人産業技術総合研究所, 主幹研究員 (20223951)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 小児内分泌学 / 生物時計 / 思春期発来メカニズム / 時計遺伝子 / Kisspeptin / 思春期発来 / GnRH遺伝子 / 概日リズム / 発光レポーター測定 |
|---|
| Research Abstract |
We established stable transformed GT-7 cell line tranfected with reporter plasmid which consists of GnRH or clock genes (Pes2/Bmal1) promoter and luciferase gene. Using this plasmid, we examined the effect of kisspepin to the transcription activity of GnRH gene or clock genes. Kisspeptin induced the transcription activity of GnRH, although it did not change that of clock genes. We also tried to establish the perfusion system for monitoring continuous luciferase activity as well as GnRH secretion. But it has not been completed.
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