| Project/Area Number |
22591144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUISHI Toyojiro 久留米大学, 医学部, 教授 (60157237)
TANAKA Eiichiro 久留米大学, 医学部, 教授 (80188284)
MURAI Yoshinaka 久留米大学, 医学部, 准教授 (40322820)
NISHI Yoshihiro 久留米大学, 医学部, 講師 (20352122)
OKABE Yasunori 久留米大学, 医学部, 助教 (00446098)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Rett 症候群 / Rett症候群 / レット症候群(RTT) / MeCP2 / iPS細胞 / 疾患モデル / 再生医学 |
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| Research Abstract |
Rett syndrome (RTT) is a neurodevelopmetal disorder associated with mutations in the methyl-CpG?binding protein 2 (MeCP2) gene. MeCP2-deficient mice recapitulate the neurological degeneration observed in RTT patients. The generation of diseased induced pluripotetn stem (iPS) cell is a critical step in understanding the pathogenesis of genetics and complex diseases. Here, we generated RTT-model mouse iPS cell lines from MeCP2-deficient mouse by co-expressing four factors (Oct3/4, Sox2, Klf4, and c-Myc) throughlentiviraldeliveryandsubsequentlydifferentiatedthemintoneuronalcellsfor further analyses. RTT-model iPS cells were characterized for the expression of pluripotent markers and for their ability to differentiate into all three germ layers by forming embryoid bodies in vitro. In addition, SDIA-induced colonies from both WT and RTT-model iPS cell lines generated cells possessing neuronal-like morphologies that expressed neuronal markers microtubule-associated protein 2 (MAP2) an neuron-specific tubulin beta type III. Our RTT-model iPS cell lines provide a powerful tool for both basic neuroscience research and therapeutic applications.
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