Histone modification change of Juvenile myelomonocytic leukemia (JMML) and establishment of JMML stem cell expansion system

• Project/Area Number: 22591154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Shinshu University
• Principal Investigator: SAKASHITA Kazuo 信州大学, 医学部附属病院, 助教 (10345746)
• Co-Investigator(Kenkyū-buntansha): KOIKE Kenich 信州大学, 医学部, 教授 (40143979)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 若年性骨髄単球性白血病 / 白血病幹細胞 / ヒストン修飾 / ヒストン / アセチル化 / JMML / ヒストンアセチル化酵素 / 白血病

Research Abstract

We established the JMML CD34+CD38- cell expansion system. This method may be useful for the development of a new therapeutic method for leukemia stem cells in JMML.And we demonstrated that histone acethyltransferase inhibitor may be useful for JMML treatment.

Research Products

• [Journal Article] Ewing sarcoma of the thoracic epidural space in a young child. (2013)
  • Author(s): Kobayashi S, Sakashita K
  • Journal Title: Eur Spine J Volume: 22 Issue: S3 Pages: 373-9
  • DOI: 10.1007/s00586-012-2481-y
  • Peer Reviewed
• [Journal Article] Successful ganciclovir therapy in a patient with human herpesvirus-6 encephalitis after unrelated cord blood transplantation: usefulness of longitudinal measurements of viral load in cerebrospinal fluid. (2013)
  • Author(s): Hirabayashi K, Sakashita K
  • Journal Title: Infection. Volume: 41 Issue: 1 Pages: 219-23
  • DOI: 10.1007/s15010-012-0329-3
  • Peer Reviewed
• [Journal Article] Replaced platelet concentrates containing a new additive solution, M-sol: safety and efficacy for pediatric patients. (2012)
  • Author(s): Yanagisawa R, Sakashita K
  • Journal Title: Transfusion. Volume: 53 Issue: 9 Pages: 2053-60
  • DOI: 10.1111/trf.12025
  • Peer Reviewed
• [Journal Article] Food allergy after cord blood transplantation in children. (2012)
  • Author(s): Sakashita K, Koike K.
  • Journal Title: Br J Haematol. Volume: 158 Issue: 5 Pages: 672-6
  • DOI: 10.1111/j.1365-2141.2012.09204.x
  • Peer Reviewed
• [Journal Article] Application of allele-specific quantitative PCR using genomic DNA to monitor minimal residual disease based on mutant gene levels following allogeneic hematopoietic stem cell transplantation in patients with hematological malignancies:comparison of mutant levels with autologous DNA percentage by short tandem repeat-PCR (2012)
  • Author(s): Taira C, Sakashita K
  • Journal Title: Clin Chim Acta Volume: 18 Issue: 3-4 Pages: 516-9
  • DOI: 10.1016/j.cca.2011.11.017
  • Peer Reviewed
• [Journal Article] Long-term haematological improvement after non-intensive or no chemotherapy in juvenile myelomonocytic leukaemia and poor correlation with adult myelodysplasia spliceosome-related mutations. (2012)
  • Author(s): Matsuda K, Sakashita K
  • Journal Title: Br J Haematol. Volume: 157 Issue: 5 Pages: 647-50
  • DOI: 10.1111/j.1365-2141.2012.09063.x
  • Peer Reviewed
• [Journal Article] Critical illness polyneuropathy and myopathy caused by Bacillus Cereus sepsis in acute lymphoblastic leukemia (2012)
  • Author(s): Hirabayashi K, Shiohara M, Suzuki T, Saito S, Tanaka M, Yanagisawa R, Tsuruta G, Fukuyama T, Hidaka Y, Nakazawa Y, Shimizu T, Sakashita K, Koike K
  • Journal Title: J Pediatr Hematol Oncol Volume: 34 Issue: 3 Pages: 110-113
  • DOI: 10.1097/mph.0b013e318234620b
  • Peer Reviewed
• [Journal Article] Genetic evaluation of childhood acute lymphoblastic leukemia in Iraq using FTA cards (2012)
  • Author(s): Al-Kzayer LF, Sakashita K, Matsuda K, Al-Hadad SA, Al-Jadiry MF, Abed WM, Abdulkadhim JM, Al-Shujairi TA, Hasan JG, Al-Abdullah HM, Al-Ani MH, Saber PA, Inoshita T, Kamata M, Koike K
  • Journal Title: Pediatr Blood Cancer Volume: 59 Issue: 3 Pages: 461-7
  • DOI: 10.1002/pbc.24055
  • Peer Reviewed
• [Journal Article] Genetic analysis of TP53 in childhood myelodysplastic syndrome and juvenile myelomonocytic leukemia. (2011)
  • Author(s): Saito S, Sakashita K
  • Journal Title: Leuk Res. Volume: 235 Issue: 12 Pages: 1578-84
  • DOI: 10.1016/j.leukres.2011.06.027
  • NAID: 120007108235
  • Peer Reviewed
• [Journal Article] Retrospective analysis of risk factors for development of liver dysfunction transient leukemia of Down syndrome (2011)
  • Author(s): Hirabayashi K, Shiohara M, Takahashi D, Saito S, Tanaka M, Yanagisawa R, Sakashita K, Nakamura T, Ishii E, Koike K
  • Journal Title: Luek Lymphoma Volume: 52 Issue: 8 Pages: 1523-1527
  • DOI: 10.3109/10428194.2011.573888
  • Peer Reviewed
• [Journal Article] A case of Behcet's disease associated with myelodysplastic syndrome involving trisomy 8 and a gain-of-function mutation in SHP-2. (2011)
  • Author(s): Shigemura T, Sakashita K
  • Journal Title: Rheumatology (Oxford) Volume: 50 Issue: 7 Pages: 1342-4
  • DOI: 10.1093/rheumatology/ker137
  • Peer Reviewed
• [Journal Article] Quantitative monitoring of single nucleotide mutations by allele-specific quantitative PCR can be used for the assessment of minimal residual disease in patients with hematological malignancies throughout their clinical course. (2011)
  • Author(s): Taira C, Sakashita K
  • Journal Title: Clin Chim Acta Volume: 14 Issue: 1-2 Pages: 53-8
  • DOI: 10.1016/j.cca.2010.09.011
  • NAID: 120007137790
  • Peer Reviewed
• [Journal Article] Disappearance of minimal residual disease after the early withdrawal of immnunosuppressants in a patient with juvenile myelomonocytic leukemia (2011)
  • Author(s): Yanagisawa R, Matsuda K, Sakashita K, Nakazawa Y, Tanaka M, Saito S, Yoshikawa K, Kamijo T, Shiohara M, Koike K
  • Journal Title: Pediatr Blood Cancer Volume: 56 Issue: 3 Pages: 166-71
  • DOI: 10.1002/pbc.22849
  • Peer Reviewed
• [Journal Article] Engraftment syndrome, but not acute GVHD, younger age, CYP3A5 or MDR1 polymorphisms, increases tacrolimus clearance in pediatric hematopoietic SCT (2011)
  • Author(s): Yanagisawa R, Katsuyama Y, Shigemura T, Saito S, Tanaka M, Nakazawa Y, Sakashita K, Shiohara M, Koike K
  • Journal Title: Bone Marrow Transplant Volume: 46 Issue: 1 Pages: 90-97
  • DOI: 10.1038/bmt.2010.64
  • Peer Reviewed
• [Journal Article] Increased pretransplant QT dispersion as a risk factor for the development of cardiac complications during and after preparative conditioning for pediatric hematopoietic stem cell transplantation (2010)
  • Author(s): Motoki N, Shimizu T, Akazawa Y, Saito S, Tanaka M, Yanagisawa R, Motoki H, Nakazawa Y, Sakashita K, Iwasaki Y, Shiohara M, Koike K
  • Journal Title: Pediatr Transplant Volume: 14 Issue: 8 Pages: 986-992
  • DOI: 10.1111/j.1399-3046.2010.01389.x
  • Peer Reviewed
• [Journal Article] Long-term survival after nonintensive chemotherapy in some juvenile myelomonocytic leukemia patients with CBL mutations, and the possible presence of healthy persons with the mutations (2010)
  • Author(s): Matsuda K, Sakashita K
  • Journal Title: Blood. Volume: 115 Issue: 26 Pages: 5429-31
  • DOI: 10.1182/blood-2009-12-260729
  • Peer Reviewed
• [Journal Article] Polymyxin-directed hemoperfusion for sepsis-induced multiple organ failure (2010)
  • Author(s): Hirabayashi K, Shiohara M, Saito S, Tanaka M, Yanagisawa R, Tsuruta G, Fukuyama T, Hidaka Y, Nakazawa Y, Shimizu T, Sakashita K, Koike K
  • Journal Title: Pediatr Blood Cancer Volume: 55 Issue: 1 Pages: 202-205
  • DOI: 10.1002/pbc.22447
  • Peer Reviewed
• [Journal Article] Primary AL amyloid polyneuropathy successfully treated with high-dose melphalan followed by autologous stem cell transplantation. (2010)
  • Author(s): Katoh N, Sakashita K
  • Journal Title: Muscle Nerve Volume: 41 Issue: 1 Pages: 138-43
  • DOI: 10.1002/mus.21483
  • Peer Reviewed
• [Journal Article] Quantitative assessment of PTPN11 or RAS mutations at the neonatal period and during the clinical course in patients with juvenile myelomonocytic leukaemia. (2009)
  • Author(s): Matsuda K, Sakashita K, Taira C, Tanaka-Yanagisawa M, Yanagisawa R, Shiohara M, Kanegane H, Hasegawa DKawasaki K, Endo M, Yajima S, Sasaki S, Kato K, Koike K, Kikuchi A, Ogawa A, Watanabe A, Sotomatsu M, Nonoyama S, Koike K
  • Journal Title: Br J Haematol Volume: 141 Issue: 4 Pages: 567-575
  • DOI: 10.1111/j.1365-2141.2009.07968.x
  • Peer Reviewed
• [Presentation] 若年性骨髄単球性白血病患者由来の iPS細胞の樹立. (2012)
  • Author(s): 重村倫成、中沢洋三、松田和之、坂下一夫、小池健一.
  • Organizer: 第54回日本小児血液・がん学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2012-11-27
• [Presentation] 小児期同種造血幹細胞移植経験者における晩期耐糖能異常の発症および危険因子 (2012)
  • Author(s): 平林香佳奈枝、 坂下一夫、 小池健一
  • Organizer: 第54回日本小児血液・がん学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2012-11-27
• [Presentation] In vitro expansion of leukemia stem cells in juvenile myelomonocytic leukemia. (2012)
  • Author(s): Kazuo Sakashita
  • Organizer: 第74回日本血液学会総会.
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19
• [Presentation] Long-term hematological improvement and poor correlation with spliceosome-related mutations in JMML. (2012)
  • Author(s): Kazuyuki Matsuda, Kazuo Sakashita
  • Organizer: 第74回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19
• [Presentation] Genetic evaluation of Iraqi pediatric acute myeloid leukemia using FTA cards. (2012)
  • Author(s): Lika'a F. Al-Kzayer, Kazuo Sakashita
  • Organizer: 第74回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19