Analysis of human sweat antigen and development of a simple diagnostic method of human sweat allergy
| Project/Area Number |
22591239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Hiroshima University |
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Principal Investigator |
MIHARA Shoji 広島大学, 大学院・医歯薬保健学研究院, 准教授 (90325168)
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| Co-Investigator(Kenkyū-buntansha) |
ISHI Kaori 広島大学, 大学院・医歯薬保健学研究院, 研究員 (90448267)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | アレルギー・ぜんそく / IgE / 汗 / アトピー性皮膚炎 / アレルギー・喘息 |
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| Research Abstract |
To identify and characterize sweat antigen which is the major aggravating factor of atopic dermatitis, human basophil histamine releasing activity in sweat was purified by a combination of chromatographies and proteins were analyzed with mass spectrometry. Some candidate peptides for sweat antigen were obtained. To measure specific IgE against sweat antigen in patients with atopic dermatitis, a simple detection system using anti-sweat monoclonal antibody and semi-purified sweat antigen was established.
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Report
(4 results)
Research Products
(14 results)
