Development of living skin equivalent models over-expressing IL-22R on epidermal keratinocytes

• Project/Area Number: 22591241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: TOHYAMA Mikiko 愛媛大学, 医学部附属病院, 助教 (60263935)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 乾癬 / IL-22 / IL-22レセプター / IL-22 レセプター / 尋常性乾癬 / STAT3 / Bcl-3 / 三次元培養皮膚 / IFN-α

Research Abstract

IL-22 is an essential cytokine in the pathogenesis of psoriasis. We have found that IL-22 receptor expression is increased in the epidermal keratinocytes of psoriasis. In this study, we elucidated that IFN-αenhances IL-22R expression in cultured keratinocytes, and that IL-22-inducible gene expression is enhanced by nuclear Bcl-3 which is increased by IL-22, particular when IL-22R is over-expressed. These findings suggest that living skin equivalent models highly-expressing IL-22R on epidermal keratinocytes may be a useful too for studying the pathogenesis of psoriasis.

Research Products

• [Journal Article] IFN-αenhances IL-22 receptor expression in keratinocyte: a possible role in development of psoriasis (2012)
  • Author(s): Tohyama M, et al
  • Journal Title: J Invest Dermatol
  • Peer Reviewed
• [Journal Article] Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents (2012)
  • Author(s): Yang L, Hashimoto K, Tohyama M, et al.
  • Journal Title: J Dermatol Sci Volume: 65 Pages: 50-57
  • Peer Reviewed
• [Journal Article] IFN-α Enhances IL-22 Receptor Expression in Keratinocytes : A Possible Role in the Development of Psoriasis (2012)
  • Author(s): Tohyama M, et al
  • Journal Title: J Invest Dermatol Volume: (印刷中) Issue: 7 Pages: 1933-1935
  • DOI: 10.1038/jid.2011.468
  • Peer Reviewed
• [Journal Article] Mite allergen is a danger signal for the skin via activation of the inflammasome in keratinocytes (2011)
  • Author(s): Dai X, Sayama K, Tohyama M, et al.
  • Journal Title: J Allergy Clin Immunol Volume: 127 Pages: 806-814
  • Peer Reviewed
• [Presentation] Nuclear translocation of Bcl3 and p50 by IL-22 producesHB-EGF, IL-8, S100A7, and human β-defensin 2 in keratinocytes of psoriasis. (2012)
  • Author(s): Tohyama M, et al.
  • Organizer: 41thannual ESDR meeting of theEuropean Society for Dermatological Research
  • Place of Presentation: Spain
  • Year and Date: 2012-12-09
• [Presentation] CISH suppresses IL-17-induced CCL20 production from epidermal keratinocytes in Th2 dominant environment. (2012)
  • Author(s): Tohyama M, et al
  • Organizer: 日本研究皮膚科学会第37回年次学術大会
  • Place of Presentation: 沖縄
  • Year and Date: 2012-12-07
• [Presentation] Nuclear translocation of Bcl3 and p50 triggers the expression of IL-22-inducible genes in keratinocytes of psoriasis (2011)
  • Author(s): Tohyama M, et al
  • Organizer: 日本研究皮膚科学会第36回年次学術大会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-09
• [Presentation] Nuclear translocation of Bcl3 and p50 triggers the expression of IL-22-inducible genes in keratinocytes of psoriasis (2011)
  • Author(s): Tohyama M
  • Organizer: 日本研究皮膚科学会第36回年次学術大会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] Involvement of IL-22 receptor enhancement by interferon alpha in psoriais (2011)
  • Author(s): Tohyama M, et al
  • Organizer: 日本研究皮膚科学会第35回年次学術大会
  • Place of Presentation: 和歌山
  • Year and Date: 2011-11-03
• [Presentation] Nuclear translocation of Bcl3 and p50 by IL-22 produces HB-EGF, IL-8, S100A7, and human ss-defensin 2 in keratinocytes of psoriasis (2011)
  • Author(s): Tohyama M
  • Organizer: 41^<th> annual ESDR meeting of the European Society for Dermatological Research
  • Place of Presentation: スペイン(招待講演)
  • Year and Date: 2011-09-10
• [Presentation] Involvement of IL-22 receptor enhancement by interferon alpha in psoriais (2010)
  • Author(s): 藤山幹子
  • Organizer: 日本研究皮膚科学会第35回年次学術大会
  • Place of Presentation: 和歌山
  • Year and Date: 2010-11-03
• [Presentation] Interferon alpha enhances IL-22 receptor expression on epidermalkeratinocytes (2010)
  • Author(s): Tohyama M, et al
  • Organizer: 40thannual ESDR meeting of the European Societyfor Dermatological Research
  • Place of Presentation: Finland
  • Year and Date: 2010-09-09
• [Presentation] Interferon alpha enhances IL-22 receptor expression on epidermal keratinocytes (2010)
  • Author(s): 藤山幹子
  • Organizer: 40^<th> annual ESDR meeting of the European Society for Dermatological Research
  • Place of Presentation: フィンランド
  • Year and Date: 2010-09-09
• [Presentation] CISH suppresses IL-17-induced CCL20 production from epidermal keratinocytes in Th2 dominant environment
  • Author(s): Tohyama M
  • Organizer: 日本研究皮膚科学会第37回年次学術大会
  • Place of Presentation: 沖縄
• [Presentation] Nuclear translocation of Bcl3 and p50 by IL-22 produces HB-EGF, IL-8, S100A7, and human β-defensin 2 in keratinocytes of psoriasis
  • Author(s): Tohyama M
  • Organizer: 41th annual ESDR meeting of the European Society for Dermatological Research
  • Place of Presentation: スペイン