| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
In order to clarify cell kinetics of Langerhans cells (LC) and adhesion molecules of keratinocytes (KC) in the epidermis, in vivo imaging was performed using the genetically engineered mouse produced by crossing the desmoglein 3-EGFP mouse and the CD11c-EYFP mouse.
In the stationary state, LC remained in the same position and an expression of desmoglein of KC in contact with LC had decreased or disappeared. In contact hypersensitivity induced by hapten application, an extension and retraction motion of dendrites of approximately 30% LC was enhanced, and 1 to 8 % of LC moved in an amoeboid motion in the epidermis. At the time of LC migration, the surrounding KC were changing the shape flexibly and the expression of desmoglein decreased along with the LC movement and recovered the expression promptly within several minutes after the LC migration.
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