Interaction of adhesion molecules of keratinocytes and Langerhans cells in the epidermis in contact hypersensitivity

• Project/Area Number: 22591252
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kanazawa Medical University
• Principal Investigator: NISHIBU Akiko 金沢医科大学, 医学部, 准教授 (30336458)
• Co-Investigator(Renkei-kenkyūsha): HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 角化細胞 / ランゲルハンス細胞 / 接触アレルギー / デスモグレイン / 接着分子

Research Abstract

In order to clarify cell kinetics of Langerhans cells (LC) and adhesion molecules of keratinocytes (KC) in the epidermis, in vivo imaging was performed using the genetically engineered mouse produced by crossing the desmoglein 3-EGFP mouse and the CD11c-EYFP mouse.
In the stationary state, LC remained in the same position and an expression of desmoglein of KC in contact with LC had decreased or disappeared. In contact hypersensitivity induced by hapten application, an extension and retraction motion of dendrites of approximately 30% LC was enhanced, and 1 to 8 % of LC moved in an amoeboid motion in the epidermis. At the time of LC migration, the surrounding KC were changing the shape flexibly and the expression of desmoglein decreased along with the LC movement and recovered the expression promptly within several minutes after the LC migration.

Research Products

• [Journal Article] Analysis of Trichophyton antigen-induced contact hypersensitivity in mouse (2012)
  • Author(s): Nakamura T, Nishibu A, Yasoshima M, Tanoue C, Yoshida N, Hatta J, Miyamoto T, Nishii M, Yanagibashi T, Nagai Y, Takatu K, Mochizuki T, Ogawa K
  • Journal Title: J Dermatol Sci Volume: 66 Pages: 144-53
  • Peer Reviewed
• [Journal Article] Analysis of Trichophyton antigen-induced contact hypersensitivity in mouse (2012)
  • Author(s): Nakamura, T., et al.
  • Journal Title: Journal of Dermatological Science Volume: (in press) Issue: 2 Pages: 144-153
  • DOI: 10.1016/j.jdermsci.2012.02.008
  • Peer Reviewed
• [Journal Article] Analysis of Trichophyton antigen-induced contact hypersensitivity in mouse (2012)
  • Author(s): Tomoya Nakamura
  • Journal Title: Journal of Dermatological Science Volume: 66 Pages: 144-153
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of Trichophyton tonsurans strains isolated in Japan between 2006 and 2010 and their susceptibility to oral antimycotics (2011)
  • Author(s): Kazushi Anzawa
  • Journal Title: Japanese Journal of Infectious Disease Volume: 64 Pages: 458-462
  • Peer Reviewed
• [Journal Article] Intravital imaging of IL-1beta production in skin (2010)
  • Author(s): Hironori Matsushima
  • Journal Title: Journal of Investigative Dermatology Volume: 130 Pages: 1571-1580
  • Peer Reviewed
• [Journal Article] Primary localized cutaneous amyloidosis in patients with scleroderma (2010)
  • Author(s): Nobuyuki Kikuchi
  • Journal Title: Acta Dermato Venereologica Volume: 90 Pages: 326-327
  • Peer Reviewed
• [Journal Article] Basal cell carcinoma on the toe (2010)
  • Author(s): Yasuhiro Suzuki
  • Journal Title: Dermatology Online Journal Volume: 16
  • Peer Reviewed