An establishment of the novel molecular imaging technique for the quantitative evaluation of the intra-tumoral apoptosis

• Project/Area Number: 22591335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Okayama University
• Principal Investigator: KURODA masahiro 岡山大学, 保健学研究科, 教授 (50225306)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: アポトーシス / MRI / 分子イメージング / ADC / 拡散強調画像 / MR

Research Abstract

The novel molecular imaging technique has been established deductively for quantitative evaluation of apoptosis using newly developed bio-phantom, which enables cellular imaging with clinical imaging devices under three-dimensional cell-culture cellular culture.
The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.

Research Products

• [Journal Article] Seiichiro Ohno and Jun‑ichi Asaumi、A new phantom and empirical formula for apparent diffusion coefficient measurement by a 3 Tesla magnetic resonance imaging scanner (2014)
  • Author(s): Marina Hara, Masahiro Kuroda, Yuichi Ohmura, Hidenobu Matsuzaki, Tomoki Kobayashi, Jun Murakami, Kazunori Katashima, Masakazu Ashida
  • Journal Title: Oncol Lett Volume: (in press)
  • Peer Reviewed
• [Journal Article] A new phantom and empirical formula for apparent diffusion coefficient measurement of a 3 tesla MRI scanner (2014)
  • Author(s): MARINA HARA, MASAHIRO KURODA, YUICHI OHMURA, HIDENOBU MATSUZAKI, TOMOKI KOBAYASHI, JUN MURAKAMI, KAZUNORI KATASHIMA, MASAKAZU ASHIDA, SEIICHIRO OHNO, and JUN-ICHI ASAUMI
  • Journal Title: Oncology Letters Volume: NA
  • Peer Reviewed
• [Journal Article] In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Jurkat Cells Using Bio-phantoms (2013)
  • Author(s): Kazunori Katashima, Masahiro Kuroda, Masakazu Ashida, Takanori Sasaki, Takehito Taguchi, Hidenobu Matsuzaki, Jun Murakami, Yoshinobu Yanagi, Miki Hisatomi, Marina Hara, Hirokazu Kato, Yuichi Ohmura, Tomoki Kobayashi, Susumu Kanazawa, Sosuke Harada, Mitsuhiro Takemoto, Seiichiro Ohno, Seiichi Mimura, and Junichi Asaumi
  • Journal Title: Acta Med Okayama Volume: 67巻 Pages: 359-367
  • NAID: 120005353347
  • Peer Reviewed
• [Journal Article] In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Jurkat Cells Using Bio-phantoms (2013)
  • Author(s): Kazunori Katashima, Masahiro Kuroda, Masakazu Ashida, Takanori Sasaki, Takehito Taguchi, Hidenobu Matsuzaki, Jun Murakami, Yoshinobu Yanagi, Miki Hisatomi, Marina Hara, Hirokazu Kato, Yuichi Ohmura, Tomoki Kobayashi, Susumu Kanazawa, Sosuke Harada, Mitsuhiro Takemoto, Seiichiro Ohno, Seiichi Mimura, and Junichi Asaumi
  • Journal Title: Acta Med Okayama Volume: 67 Pages: 359-367
  • NAID: 120005353347
  • Peer Reviewed
• [Journal Article] In vitro assessment of factors affecting the apparent diffusion coefficient of Ramos cells using bio-phantoms (2012)
  • Author(s): Sasaki T, Kuroda M, Katashima K, Ashida M, Matsuzaki H, Asaumi J, Murakami J, Ohno S, Kato H and Kanazawa S
  • Journal Title: Acta Med Okayama Volume: 66巻 Pages: 263-270
  • NAID: 120004247097
  • Peer Reviewed
• [Journal Article] In vitro assessment of factors affecting the apparent diffusion coefficient of Ramos cells using bio-phantoms. (2012)
  • Author(s): Sasaki T, Kuroda M, Katashima K, Ashida M, Matsuzaki H, Asaumi J, Murakami J, Ohno S, Kato H and Kanazawa S
  • Journal Title: Acta Med Okayama Volume: 66 Pages: 263-270
  • NAID: 120004247097
  • Peer Reviewed
• [Presentation] In vitro assessment of change of the apparent diffusion coefficient of Jurkat cells after heating using bio-phantoms and MRI (2012)
  • Author(s): Masahiro Kuroda, Kazunori Katashima, Masakazu Ashida, Susumu Kanazawa, Shoji Kawasaki, Hirokazu Kato
  • Organizer: The 11th International Congress of Hyperthermic Oncology & The 29th Japanese Congress of Thermal Medicine
  • Place of Presentation: Kyoto
  • Year and Date: 2012-08-29
• [Presentation] In vitro assessment of change of the apparent diffusion coefficient of Jurkat cells after heating using bio-phantoms and MRI (2012)
  • Author(s): Masahiro Kuroda, Kazunori Katashima, Masakazu Ashida, Susumu Kanazawa, Shoji Kawasaki, Hirokazu Kato
  • Organizer: The 11th International Congress of Hyperthermic Oncology
  • Place of Presentation: Kyoto
• [Presentation] 腫瘍のADC値の成因と治療後変化のメカニズムに関するバイオファントムを用いた検討 (2010)
  • Author(s): 黒田昌宏、佐々木崇了、片嶋和典、芦田昌和、田口勇仁、金澤右、浅海淳一、松崎秀信、加藤博和
  • Organizer: 第115回日本医学放射線学会中国・四国地方会
  • Place of Presentation: 下関市
  • Year and Date: 2010-12-17
• [Presentation] 腫瘍のADC値の成因と治療後変化のメカニズムに関するバイオファントムを用いた検討 (2010)
  • Author(s): 黒田昌宏、佐々木崇了、片嶋和典、芦田昌和、田口勇仁、金澤右、浅海淳一、松崎秀信、加藤博和
  • Organizer: 第115回 日本医学放射線学会中国・四国地方会
  • Place of Presentation: 下関市
  • Year and Date: 2010-12-17
• [Presentation] バイオファントムを用いたADC値の成因の基礎検討(1)-撮像法の基礎的検討と細胞密度によるADC値の変化- (2010)
  • Author(s): 芦田昌和、黒田昌宏、佐々木崇了、片嶋和典、田口勇仁、金澤右、浅海淳一、松崎秀信、大野誠一郎、三村誠一、北山卓一、大川義弘、赤木憲明、稲村圭司、加藤博和
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-10-01
• [Presentation] バイオファントムを用いたADC値の成因の基礎検討(2)-加温処理後のADC値の変化- (2010)
  • Author(s): 片嶋和典、黒田昌宏、芦田昌和、佐々木崇了、田口勇仁、金澤右、浅海淳一、松崎秀信、大野誠一郎、三村誠一、北山卓一、大川義弘、赤木憲明、稲村圭司、加藤博和
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-10-01
• [Presentation] バイオファントムを用いたADC値の成因の基礎検討(3)-分子標的薬剤によるアポトーシス誘導時のADC値の変化- (2010)
  • Author(s): 佐々木崇了、黒田昌宏、片嶋和典、芦田昌和、田口勇仁、金澤右、浅海淳一、松崎秀信、大野誠一郎、三村誠一、北山卓一、大川義弘、赤木憲明、稲村圭司、加藤博和
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-10-01
• [Presentation] バイオファントムを用いたADC値の成因の基礎検討(1)-撮像法の基礎的検討と細胞密度によるへADC値の変化- (2010)
  • Author(s): 芦田昌和、黒田昌宏、佐々木崇了、片嶋和典、田口勇仁、金澤右、浅海淳一、松崎秀信、大野誠一郎、三村誠一、北山卓一、大川義弘、赤木憲明、稲村圭司、加藤博和
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-10-01
• [Presentation] Fundamental research regarding the factors influencing ADC in MR imaging using a bio-phantom - Basic examination of the MR imaging procedure and the changes in ADC values with cellularity - (2010)
  • Author(s): Masakazu Ashida, Masahiro Kuroda, Kazunori Katashima, Takanori Sasaki, Junichi Asaumi
  • Organizer: The 8th Asian Congress of Oral and Maxillofacial Radiology
  • Place of Presentation: Seoul (Korea)
• [Presentation] Basic examination of ADC values using a bio-phantom - The change in ADC values after heating - (2010)
  • Author(s): Kazunori Katashima, Masahiro Kuroda, Takanori Sasaki, Masakazu Ashida, Junichi Asaumi
  • Organizer: The 8th Asian Congress of Oral and Maxillofacial Radiology
  • Place of Presentation: Seoul (Korea)