Detection of novel cancer-related genes by using triple combination array analysis

• Project/Area Number: 22591427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nagoya University
• Principal Investigator: NOMOTO Shuji 名古屋大学, 医学部附属病院, 病院講師 (40300967)
• Co-Investigator(Kenkyū-buntansha): KASUYA Hideki 名古屋大学, 大学院・医学系研究科, 准教授 (00402636) ; NAKAO Akimasa 名古屋大学, 大学院・医学系研究科, 教授 (70167542)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: トリプルアレイ法 / 発現アレイ / SNP アレイ / メチレーションアレイ / 消化器癌 / SNPアレイ / トリプルアレイ / メチル化アレイ / 肝細胞癌 / 胃癌 / がん関連遺伝子 / バイオマーカー

Research Abstract

It has been known that cancer is a disease associated with the genetic abnormality. Various array technologies have been used to extract genes which were related tumor initiation and progression. We have devised a triple combination array analysis which was consisted the expression array, the SNP array, and the methylation array to identify tumor related genes. We could predict prognosis and/or recurrence using some of the extracted genes.

Research Products

• [Journal Article] Identification of the A Kinase Anchor Protein 12 (AKAP12) Gene as a Candidate Tumor Suppressor of Hepatocellular Carcinoma (2012)
  • Author(s): Masamichi Hayashi, Shuji Nomoto, Mitsuro Kanda, Yukiyasu Okamura, Yoko Nishikawa, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Shin Takeda, Yasuhiro Kodera
  • Journal Title: Journal of Surgical Oncology Volume: 105(4) Pages: 381-386
  • DOI: 10.1002/jso.22135
  • Peer Reviewed
• [Journal Article] Suppressor of Cytokine Signaling 4 Detected as a Novel Gastric Cancer Suppressor Gene using Double Combination Array Analysis (2012)
  • Author(s): Daisuke Kobayashi, Shuji Nomoto, Yasuhiro Kodera, Michitaka Fujiwara, Masahiko Koike, Goro Nakayama, Norifumi Ohashi, Akimasa Nakao
  • Journal Title: World Journal of Surgery Volume: 36(2) Pages: 362-372
  • DOI: 10.1007/s00268-011-1358-2
  • Peer Reviewed
• [Journal Article] Identification of the bleomycin hydrolase gene as a methylated tumor suppressor gene in hepatocellular carcinoma using a novel triple-combination array method (2011)
  • Author(s): Yukiyasu Okamura, Shuji Nomoto, Masamichi Hayashi, Mitsuhiro Hishida, Yoko Nishikawa, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Shin Takeda, Yasuhiro Kodera, Akimasa Nakao
  • Journal Title: Cancer Letters Volume: 312(2)
  • DOI: 10.1016/j.canlet.2011.07.028
  • Peer Reviewed
• [Journal Article] Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma. (2011)
  • Author(s): Mitsuro Kanda, Shuji Nomoto, Yukiyasu Okamura, Masamichi Hayashi, Mitsuhiro Hishida, Tsutomu Fujii, Yoko Nishikawa, Hiroyuki Sugimoto, Shin Takeda, Akimasa Nakao
  • Journal Title: Molecular Carcinogenesis Volume: 50(8) Pages: 571-579
  • DOI: 10.1002/mc.20735
  • Peer Reviewed
• [Journal Article] Reduced Expression of Reelin (RELN) Gene Is Associated With High Recurrence Rate of Hepatocellular Carcinoma (2011)
  • Author(s): Yukiyasu Okamura, Shuji Nomoto, Mitsuro Kanda, Masamichi Hayashi, Yoko Nishikawa, Tsutomu Fujii, Hiroyuki Sugimoto, Shin Takeda, Akimasa Nakao
  • Journal Title: Annals of Surgical Oncology Volume: 18(2) Pages: 572-579
  • DOI: 10.1245/s10434-010-1273-z
  • Peer Reviewed
• [Journal Article] Identification of the bleomycin hydrolase gene as a methylated tumor suppressor gene in hepatocellular carcinoma using a novel triple-combination array method (2011)
  • Author(s): Okamura Y, Nomoto S, Hayashi M, Hishida M, Nishikawa Y, Yamada S, Fujii T, Sugimoto H, Takeda S, Kodera Y, Nakao A
  • Journal Title: Cancer Letter Volume: Dec 22:312(2) Pages: 150-7
  • Peer Reviewed
• [Presentation] トリプルアレイ法による新規肝細胞癌関連遺伝子, CCNJ 遺伝子の抽出 (2012)
  • Author(s): 野本周嗣,林 真路,菱田光洋,猪川祥邦,小寺泰弘
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山 (富山県民会館)
  • Year and Date: 2012-07-19
• [Presentation] トリプルアレイ法により抽出した HCC の新規癌関連遺伝子,DCDC2 の検討 (2012)
  • Author(s): 猪川祥邦,野本周嗣,林 真路,菱田光洋,小寺泰弘
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山 (富山県民会館)
  • Year and Date: 2012-07-19
• [Presentation] トリプルアレイ法を用い検出された PTK7 遺伝子の,肝細胞癌における発現の検討 (2012)
  • Author(s): 菱田光洋,野本周嗣,猪川祥邦,林 真路,山田 豪,藤井 努,杉本博行,竹田 伸,小寺泰弘
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山 (富山県民会館)
  • Year and Date: 2012-07-19
• [Presentation] 肝細胞癌におけるトリプルアレイ法による癌関連遺伝子の検索 ~COL1A1 遺伝子の解析~ (2012)
  • Author(s): 林 真路,野本周嗣,菱田光洋,猪川祥邦,山田 豪,藤井 努,杉本博行,竹田 伸,小寺泰弘
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉 (幕張メッセ)
  • Year and Date: 2012-04-13
• [Presentation] トリプルアレイ法を用いた新規肝細胞癌関連遺伝子 STEP4 の抽出 (2012)
  • Author(s): 野本周嗣,林 真路,菱田光洋,猪川祥邦,山田 豪,藤井 努,杉本博行,竹田 伸,小寺泰弘
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉 (幕張メッセ)
  • Year and Date: 2012-04-12
• [Presentation] Use of Triple Combination Array Analysis to Indentify Steap Family Member 4 (STEAP4), A Novel Tumor Related Gene in Hepatocellular Carcinoma (2012)
  • Author(s): Shuji Nomoto, Mitsuhiro Hishida, Yoshikkuni Inokawa, Yasuhiro Kodera
  • Organizer: 10th International Conference of The Asian Clinical Oncology Society
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2012-01-14
• [Presentation] Availability of a Newly Designed Technique, Triple-Combination Array Analysis: Identification of Estrogen Receptor 1 Gene in Hepatocelullar Carcinoma Using This Method (2012)
  • Author(s): Mitsuhiro Hishida, Shuji Nomoto, Yoshikkuni Inokawa, Yasuhiro Kodera
  • Organizer: 10th International Conference of The Asian Clinical Oncology Society
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2012-01-14
• [Presentation] Doublecortin Domain Containing 2 (DCDC2) Gene, A New Candidate of Tumor Suppressor Gene of Hepatocellular Carcinoma Detected by Triple Combination Array Analysis (2012)
  • Author(s): Yoshikkuni Inokawa, Shuji Nomoto, Mitsuhiro Hishida, Yasuhiro Kodera
  • Organizer: 10th International Conference of The Asian Clinical Oncology Society
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2012-01-14
• [Presentation] トリプルアレイ法を用いたHCC 新規癌関連遺伝子,STEAP4,の同定 (2011)
  • Author(s): 野本周嗣,岡村行泰,林 真路,菱田光洋,竹田 伸,杉本博行,藤井 努,山田 豪.小寺泰弘
  • Organizer: 第49回日本癌治療学会学術集会
  • Place of Presentation: 名古屋 (名古屋国際会議場)
  • Year and Date: 2011-10-27
• [Presentation] トリプルアレイ法を用いた肝細胞癌における新規癌関連遺伝子検出の試み-Bleomycin hydrolase(BLMH)遺伝子に関する検討- (2011)
  • Author(s): 岡村行泰,野本周嗣,林 真路,菱田光洋,藤井 努,杉本博行,竹田 伸,中尾昭公
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 震災のため紙上開催
  • Year and Date: 2011-05-26
• [Presentation] EFEMP1,a novel tumor suppressor gene detected in hepatocellular carcinoma using double combination array analysis (2010)
  • Author(s): Shuji Nomoto, Shin Takeda, Hideki Kasuya, Yasuhiro Kodera, Michitaka Fujiwara, Masahiko Koike, Akimasa Nakao
  • Organizer: 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪 (リーガロイヤルホテル大阪)
  • Year and Date: 2010-09-22