Identification and functional analysis of esophageal cancer-related genes by pull-down method using biotin-labeled miRNA
| Project/Area Number |
22591433
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Okayama University |
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Principal Investigator |
ITO Sachio 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30335624)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Takashi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50160651)
OUCHIDA Mamoru 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80213635)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 食道癌 / miRNA / microRNA / pull-down assay / 癌 |
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| Research Abstract |
In order to identify possible predictive markers, We performed the identification of miRNA related to the degree of differentiation-grade and malignancy by the profiling of esophageal cancer cell lines and esophageal cancer clinical specimens. In poorly differentiated cells, miR-29a and let-7b were overexpressed. We identified target genes of these miRNAs using in vitro pull-down method, and found some candidates of target genes related to tumor suppressor and apoptosis. Our results suggest that these miRNAs are involved in esophageal cancer progression.
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Report
(4 results)
Research Products
(23 results)
