| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We inoculated subcutaneously NR-S1M metastatic cells into the backs of C3H/HeN mice. Concurrently, 1 μg of recombinant mouse CRP or PBS were injected subcutaneously every 3 days for 5 weeks, after which the mice were killed for evaluation. We evaluated lymph node metastasis and lymphangiogenesis in the implanted tumor using immunohistochemical analysis with anti-lymphatic vessel endothelial hyaluronan receptor-1 antibodies. Immunohistochemical analysis confirmed inguinal lymph node metastasis in 70% of control mice, but in only 30% of mice in the CRP group. Moreover, tumoral lymphangiogenesis was decreased in the CRP group). CRP appears to inhibit tumoral lymphangiogenesis and lymph node metastasis in mice.
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