Breakthrough of mechanism in micrometastasis using micrometastatic model of esophageal cancer

• Project/Area Number: 22591450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Gunma University
• Principal Investigator: MIYAZAKI Tatsuya 群馬大学, 医学部附属病院, 助教 (70372349)
• Co-Investigator(Kenkyū-buntansha): YOKOBORI Takehiko 群馬大学, 医学系研究科, 助教 (60420098) ; SAKAI Makoto 群馬大学, 医学部附属病院, 助教 (70420099) ; SOHDA Makoto 群馬大学, 医学部附属病院, 助教 (70507326) ; KUWANO Hiroyuki 群馬大学, 医学系研究科, 教授 (90186560) ; 田中 成岳 群馬大学, 医学部, 助教 (30546726) ; 猪瀬 崇徳 群馬大学, 医学部, 助教 (90609968)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 微小転移 / 腹膜播種 / 癌 / 食道癌 / 同所移植モデル / 同所移植転移モデル微小転移 / 同所移植転移モデル

Outline of Final Research Achievements

We have established mice model to evaluate peritoneal micro metastatic nest, because we could not establish orthotopic transplantation model. We used colon-26-luc cell line and 7-week-old female Balb-c mice. We could visualize micro peritoneal cancer nest as a light of luciferase. We also measured the weight of peritoneal tissue to quantify tumor volume, that related luminescence intensity. We establish the method to evaluate disease progression without sacrifice of mice.

Research Products

• [Journal Article] Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi. (2016)
  • Author(s): Yokobori T, Suzuki S, Miyazaki T, Sohda M, Sakai M, Tanaka N, Ozawa D, Hara K, Honjo H, Altan B, Fukuchi M, Ishii H, Iwatsuki M, Sugimachi K, Sudo T, Iwaya T, Nishida N, Mimori K, Kuwano H, Mori M.
  • Journal Title: Dis. Esophagus. Volume: [Epub ahead of print] Pages: 843-847
  • DOI: 10.1111/dote.12346
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phase II Study of Docetaxel, Nedaplatin, and 5-Fluorouracil Combined Chemotherapy for Advanced Esophageal Cancer. (2015)
  • Author(s): Miyazaki T, Ojima H, Fukuchi M, Sakai M, Sohda M, Tanaka N, Suzuki S, Ieta K, Saito K, Sano A, Yokobori T, Inose T, Nakajima M, Kato H, Kuwano H.
  • Journal Title: Ann Surg Oncol. Volume: [Epub ahead of print]
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phase I/II study of docetaxel, cisplatin, and 5-fluorouracil combination chemoradiotherapy in patients with advanced esophageal cancer. (2015)
  • Author(s): Miyazaki T, Sohda M, Tanaka N, Suzuki S, Ieta K, Sakai M, Sano A, Yokobori T, Inose T, Nakajima M, Fukuchi M, Ojima H, Kato H, Kuwano H.
  • Journal Title: Cancer Chemother Pharmacol. Volume: 75 Pages: 449-55
  • DOI: 10.1007/s00280-014-2659-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low PHLDA3 expression in oesophageal squamous cell carcinomas is associated with poor prognosis. (2015)
  • Author(s): Muroi H, Nakajima M, Satomura H, Takahashi M, Yamaguchi S, Sasaki K, Yokobori T, Miyazaki T, Kuwano H, Kato H.
  • Journal Title: Anticancer Res. Volume: 35 Pages: 949-54
  • Peer Reviewed
• [Journal Article] Surgical Resection of a Solitary Pulmonary Metastasis from Basaloid Squamous Cell Carcinoma of the Esophagus: A Case Report (2014)
  • Author(s): Kosaka T, Mogi A, Yamaki E, Miyazaki T, Kuwano H.
  • Journal Title: ATCS Volume: Epub ahead of print Issue: Supplement Pages: 646-649
  • DOI: 10.5761/atcs.cr.13-00100
  • NAID: 130003387984
  • Year and Date: 2014-12-19
  • Peer Reviewed
• [Journal Article] TGFBI Expression in Cancer Stromal Cells is Associated with Poor Prognosis and Hematogenous Recurrence in Esophageal Squamous Cell Carcinoma. (2014)
  • Author(s): Ozawa D, Yokobori T, Sohda M, Sakai M, Hara K, Honjo H, Kato H, Miyazaki T, Kuwano H.
  • Journal Title: Ann Surg Oncol. Volume: Epub ahead of print
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] L-[3-18F]-α-methyltyrosine uptake by lymph node metastasis is a predictor of complete response to CRT in esophageal cancer. (2014)
  • Author(s): Sohda M, Miyazaki T, Honjyo H, Hara K, Ozawa D, Suzuki S, Tanaka N, Sano A, Sakai M, Yokobori T, Nakajima M, Fukuchi M, Kato H, Higuchi T, Tsushima Y, Kuwano H.
  • Journal Title: Anticancer Res. Volume: 34 Pages: 7473-7
  • Peer Reviewed
• [Journal Article] Salvage lymphadenectomy for recurrent esophageal cancer after chemoradiotherapy. (2014)
  • Author(s): Nakajima M, Domeki Y, Satomura H, Takahashi M, Sugawara A, Muroi H, Sasaki K, Yamaguchi S, Miyazaki T, Kuwano H, Kato H.
  • Journal Title: Int Surg. Volume: 99 Pages: 452-7
  • DOI: 10.9738/intsurg-d-13-00261.1
  • Peer Reviewed
• [Journal Article] Use of pre-treatment 18F-FAMT PET to predict patient survival in squamous cell carcinoma of the esophagus treated by curative surgery. (2014)
  • Author(s): Sohda M, Sakai M, Honjyo H, Hara K, Ozawa D, Suzuki S, Tanaka N, Yokobori T, Miyazaki T, Fukuchi M, Higuchi T, Tsushima Y, Kuwano H.
  • Journal Title: Anticancer Res. Volume: 34 Pages: 3623-8
  • Peer Reviewed
• [Journal Article] Biological significance of fluorine-18-α-methyltyrosine (FAMT) uptake on PET in patients with oesophageal cancer. (2014)
  • Author(s): Suzuki S, Kaira K, Ohshima Y, Ishioka NS, Sohda M, Yokobori T, Miyazaki T, Oriuchi N, Tominaga H, Kanai Y, Tsukamoto N, Asao T, Tsushima Y, Higuchi T, Oyama T, Kuwano H.
  • Journal Title: Br J Cancer. Volume: Epub ahead of print Pages: 1985-1991
  • DOI: 10.1038/bjc.2014.142
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] L-[3-18F]-α-methyltyrosine accumulation as a definitive chemoradiotherapy response predictor in patients with esophageal cancer. (2014)
  • Author(s): Sohda M, Honjyo H, Hara K, Ozawa D, Suzuki S, Tanaka N, Sano A, Sakai M, Yokobori T, Inose T, Miyazaki T, Ojima H, Higuchi T, Tsushima Y, Kuwano H.
  • Journal Title: Anticancer Res Volume: 34(2) Pages: 909-13
  • Peer Reviewed
• [Journal Article] Effectiveness of FDG-PET in screening of synchronous cancer of other organs in patients with esophageal cancer. (2014)
  • Author(s): Miyazaki T, Sohda M, Higuchi T, Tanaka N, Suzuki S, Sakai M, Yokobori T, Nakajima M, Fukuchi M, Tsushima Y, Kato H, Kuwano H.
  • Journal Title: Anticancer Res Volume: 34(1) Pages: 283-7
  • Peer Reviewed
• [Journal Article] FBXW7 mediates chemotherapeutic sensitivity and prognosis in NSCLCs (2014)
  • Author(s): Yokobori T, Yokoyama Y, Mogi A, Endoh H, Altan B, Kosaka T, Yamaki E, Yajima T, Tomizawa K, Azuma Y, Onozato R, Miyazaki T, Tanaka S, Kuwano H.
  • Journal Title: Mol Cancer Res. Volume: 12(1) Pages: 32-37
  • DOI: 10.1158/1541-7786.mcr-13-0341
  • Peer Reviewed
• [Journal Article] Clinical significance of total colonoscopy for screening of colon lesions in patients with esophageal cancer. (2013)
  • Author(s): Miyazaki T, Tanaka N, Sano A, Suzuki S, Sakai M, Yokobori T, Inose T, Sohda M, Nakajima M, Fukuchi M, Ojima H, Kato H, Kuwano H.
  • Journal Title: Anticancer Res Volume: 33(11) Pages: 5113-7
  • Peer Reviewed
• [Journal Article] Re-thoracotomy for intrathoracic complications after esophagectomy for esophageal cancer (2013)
  • Author(s): Miyazaki T, Inose T, Tanaka N, Suzuki S, Hara K, Ozawa D, Yokobori T, Sakai M, Souda M, Fukuchi M, Kuwano H.
  • Journal Title: Kyobu Geka Volume: 66(8 Suppl) Pages: 762-6
  • NAID: 40019746006
• [Journal Article] Expression of receptor tyrosine kinases in esophageal carcinosarcoma. (2013)
  • Author(s): Sano A
  • Journal Title: Oncol Rep. Volume: 29 Pages: 2119-26
  • DOI: 10.3892/or.2013.2371
  • Peer Reviewed
• [Journal Article] Phase I dose-escalation study of docetaxel, nedaplatin, and 5-fluorouracil combination chemotherapy in patients with advanced esophageal cancer. (2013)
  • Author(s): Miyazaki T
  • Journal Title: Cancer Chemother Pharmacol. Volume: 71 Pages: 853-7
  • DOI: 10.1007/s00280-013-2076-2
  • Peer Reviewed
• [Journal Article] Management of Barrett's esophageal carcinoma. (2013)
  • Author(s): Miyazaki T
  • Journal Title: Surg Today Volume: 43 Pages: 353-60
  • DOI: 10.1007/s00595-012-0468-2
  • NAID: 10031168019
  • Peer Reviewed
• [Journal Article] MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1. (2013)
  • Author(s): Yokobori T
  • Journal Title: Cancer Sci. Volume: 104 Pages: 48-54
  • DOI: 10.1111/cas.12030
  • Peer Reviewed
• [Journal Article] CD47 expression regulated by the miR-133a tumor suppressor is a novel prognostic marker in esophageal squamous cell carcinoma. (2012)
  • Author(s): Suzuki S
  • Journal Title: Oncol Rep. Volume: 28 Pages: 465-72
  • DOI: 10.3892/or.2012.1831
  • Peer Reviewed
• [Journal Article] Significance of lymph node capsular invasion in esophageal squamous cell carcinoma. (2012)
  • Author(s): Sakai M
  • Journal Title: Ann Surg Oncol. Volume: 19 Pages: 1911-7
  • DOI: 10.1245/s10434-012-2232-7
  • Peer Reviewed
• [Journal Article] Ghrelin Level and Body Weight Loss After Esophagectomy for Esophageal Cancer (2011)
  • Author(s): Miyazaki T, Tanaka N, Hirai H, Yokobori T, Sano A, Sakai M, Inose T, Sohda M, Nakajima M, Fukuchi M, Kato H, Kuwano H
  • Journal Title: J Surg Res. Volume: (Epub ahead of print)
  • DOI: 10.1016/j.jss.2011.09.016
  • Peer Reviewed
• [Journal Article] TNFAIP8 Overexpression: Clinical Relevance to Esophageal Squamous Cell Carcinoma (2011)
  • Author(s): Hadisaputri YE, Miyazaki T, Suzuki S, Yokobori T, Kobayashi T, Tanaka N, Inose T, Sohda M, Kuwano H
  • Journal Title: Ann Surg Oncol Volume: (Epub ahead of print)
  • DOI: 10.1245/s10434-011-2097-1
  • Peer Reviewed
• [Journal Article] Prognostic significance of heat shock protein 110 expression and T lymphocyte infiltration in esophageal cancer (2011)
  • Author(s): Nakajima M, Kato H, Miyazaki T, Fukuchi M, Masuda N, Fukai Y, Sohda M, Inose T, Sakai M, Sano A, Tanaka N, Ahmad F, Kuwano H
  • Journal Title: Hepatogastroenterology Volume: 58 Pages: 1555-1560
  • Peer Reviewed
• [Presentation] ルシフェラーゼアッセイを利用した肉眼的に認識できない微小腹膜播種マウスモデルの確立 (2015)
  • Author(s): 横堀武彦、高橋遼、金泉、浅尾高行、桑野博行
  • Organizer: 第115回日本外科学会学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-04-16 – 2015-04-18
• [Presentation] A new low-cost and high-quality device for in vivo luciferase assay in peritoneal dissemination mouse model (2014)
  • Author(s): Takehiko Yokobori, Ryo Takahashi, Yuji Kumakura, Hiroaki Honjo, Keigo Hara, Makoto Sakai, Makoto Sohda, Tatsuya Miyazaki, Takayuki Asao, Hiroyuki Kuwano
  • Organizer: The 73rd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27