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Human Peritoneal Mesothelial Cell play important roles in the development of peritoneal metastasis by its EMT change

Research Project

Project/Area Number 22591451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKanazawa University

Principal Investigator

FUSHIDA Sachio  金沢大学, 大学病院, 講師 (10301194)

Co-Investigator(Kenkyū-buntansha) HARADA Shinichi  金沢大学, 医学系, 助教 (90272955)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords胃癌 / 腹膜播種 / 腹膜中皮細胞 / EMT / 微小環境 / 形質 転換 (EMT) / TGFβ / パクリタキセル / fibrocyte / 胃癌腹膜播種 / 形質転換 / 線維化 / paclitaxel / TGF-β
Research Abstract

We investigated whether TGF-β derived from cancer cells in the peritoneal microenvironment activates human peritoneal mesothelial cells (HPMCs), leading to the progression and fibrosis of gastric cancer. We found that activated HPMCs (a-HPMCs) became more invasive and up-regulated proliferation of human gastric cancer-derived MKN45 cells following direct cell-cell contact. These EMT-like changesof HPMC were suppressed under 5nM of paclitaxel pretreatment by inhibiting Smad2 phosphorylation. To measure the effects of co-culture in vivo, we developed a mouse xenograft model. The largest tumors were observed in mice given MKN45 cells that had been co-cultured with a-HPMCs. Additionaly, these tumors contained HPMC-derived fibrous tissue.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (10 results)

All 2013 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Low-dose paclitaxel modulates tumour fibrosis in gastric cancer2013

    • Author(s)
      Tsukada T, Fushida S
    • Journal Title

      Int J Oncol

      Volume: 42 Issue: 4 Pages: 1167-74

    • DOI

      10.3892/ijo.2013.1801

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of human peritoneal mesothelial cells in the fibrosis and progression of gastric cancer2012

    • Author(s)
      Tsukada T
    • Journal Title

      Int J Oncol

      Volume: 41 Issue: 2 Pages: 476-82

    • DOI

      10.3892/ijo.2012.1490

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] スキルス胃癌の病態と集学的治療の現況と将来展望2013

    • Author(s)
      伏田幸夫他
    • Organizer
      第85回日本胃癌学会総会
    • Place of Presentation
      阪国際会議場(大阪府)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] スキルス胃癌の病態と集学的治療の現況と将来展望2013

    • Author(s)
      伏田幸夫
    • Organizer
      第85回日本胃癌学会総会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 腹腔内微小環境下における線維化メカニズムの解明とその制御2012

    • Author(s)
      伏田幸夫他
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2012-04-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 腹腔内微小環境下における線維化メカニズムの解明とその制御2012

    • Author(s)
      伏田幸夫
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉)
    • Year and Date
      2012-04-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Interaction of gastric cancer cell and bone-marrow derived mesenchymal cell; fibrocyte2011

    • Author(s)
      Fushida S
    • Organizer
      19thUnited European Gastroenterology Week
    • Place of Presentation
      STOCKHOLM INTERNATIONAL FAIRS(Sweden)
    • Year and Date
      2011-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of mesenchymal transition of human peritoneal mesothelial cell and cell-cell interaction with gastric cancer cell in the microenvironment of peritoneal metastasis2010

    • Author(s)
      Fushida S
    • Organizer
      69thAnnual Meeting of Japanese Cancer Association
    • Place of Presentation
      Osaka International Convention Center Corp(Osaka)
    • Year and Date
      2010-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of mesenchymal transition of human peritoneal mesothelial cell and cell-cell interaction with gastric cancer cell in the microenvironment of peritoneal metastasis2010

    • Author(s)
      伏田幸夫
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Book] スキルス胃癌-基礎と臨床2010

    • Author(s)
      伏田幸夫共
    • Publisher
      医薬ジャーナル社
    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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