Human Peritoneal Mesothelial Cell play important roles in the development of peritoneal metastasis by its EMT change
| Project/Area Number |
22591451
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Shinichi 金沢大学, 医学系, 助教 (90272955)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 胃癌 / 腹膜播種 / 腹膜中皮細胞 / EMT / 微小環境 / 形質 転換 (EMT) / TGFβ / パクリタキセル / fibrocyte / 胃癌腹膜播種 / 形質転換 / 線維化 / paclitaxel / TGF-β |
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| Research Abstract |
We investigated whether TGF-β derived from cancer cells in the peritoneal microenvironment activates human peritoneal mesothelial cells (HPMCs), leading to the progression and fibrosis of gastric cancer. We found that activated HPMCs (a-HPMCs) became more invasive and up-regulated proliferation of human gastric cancer-derived MKN45 cells following direct cell-cell contact. These EMT-like changesof HPMC were suppressed under 5nM of paclitaxel pretreatment by inhibiting Smad2 phosphorylation. To measure the effects of co-culture in vivo, we developed a mouse xenograft model. The largest tumors were observed in mice given MKN45 cells that had been co-cultured with a-HPMCs. Additionaly, these tumors contained HPMC-derived fibrous tissue.
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Report
(4 results)
Research Products
(10 results)
