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Analysis of novel tumor suppressor gene RUNX3 of gastric cancer and clinical application for the diagnosis and prevention of gastric cancer

Research Project

Project/Area Number 22591463
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SAKAKURA Chouhei  京都府立医科大学, 医学研究科, 准教授 (10285257)

Co-Investigator(Kenkyū-buntansha) OKUDA Tsukasa  京都府立医科大学, 医学研究科, 教授 (30291587)
CHIBA Tsutomu  京都大学医学部, 医学研究科, 教授 (30188487)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords胃癌 / 癌抑制遺伝子 / RUNX3
Research Abstract

Based on the analysis result of RUNX3 knockout mouse as well as clinical samples of gastric cancer, the following was developed aiming at the functional analysis of RUNX3, and gene diagnosis and the application to carcinogenic prevention and medical treatment. We have already identified several kinds of markers of a gastric cancer precursor cell, and are looking for a stomach cancer precursor cell in the background membrane of an early frequent occurrence stomach cancer excision specimen. Moreover, clinical application of novel HDAC inhibitor FK228, SAHA, etc. has being carried out. We are examining a carcinogenic inhibiting effect with a mouse chemical carcinogenesis model, and considered that it can applied for the cancer prevention using RUNX3 as the molecular target. Furthermore, RUNX3 has played the role important for TGFbeta dependence apoptosis, and participating in the susceptibility of a radiation chemistry treatment became clear (Oncogene, Sakakura et al., 2007). Moreover, we are checking generating of a large intestine pathological change by crossing of RUNX3 knockout mouse and an APC knockout mouse. The molecule (TCF4) which the carcinogenic signal transfer system (Wnt signal system) of colorectal cancer and a RUNX3-TGFbeta signal transfer system overlap is also identified (Cancer Cell, Ito et al, 2009), and the further development can expect from now on. With Luminex system, we establish the quick serological-diagnosis system which can measure quantitatively methylation of two or more genes in many samples in a short time, and are trying for clinical application.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (35 results)

All 2013 2012 2011 2010 Other

All Journal Article (23 results) (of which Peer Reviewed: 22 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] COX-2 overexpression Induced by gene transfer reduces sensitivity of TE13 esophageal carcinoma cells to 5-fluorouracil and cisplatin2013

    • Author(s)
      Okamura H, Fujiwara H, Umehara S, Okamura S, Todo M, Furutani A, Yoneda M, Shiozaki A, Komatsu S, Kubota T, Ichikawa D, Okamoto K, Ochiai T, Sakakura C, Takahashi Y, Yoshimoto T, Otsuji E
    • Journal Title

      Anticancer Res

      Volume: 33 Pages: 537-542

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total pancreatectomy for pancreatic carcinoma: evaluation of safety and efficacy.2012

    • Author(s)
      M. Kitagawa, H. Ikoma, T. Ochiai, H. Ishii, A. Shiozaki, Y. Kuriu, M. Nakanishi, D. Ichikawa, K. Okamoto, H. Fujiwara,C. Sakakura, Y. Kokuba, T. Sonoyama, E. Otsuji
    • Journal Title

      Hepatogastroenterology

      Volume: 59 Pages: 907-910

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] REGIV as a potential biomarker for peritoneal dissemination in gastric adenocarcinoma.2012

    • Author(s)
      Moon JH, Fujiwara Y, Nakamura Y, Okada K, Hanada H, Sakakura C, Takiguchi S, Nakajima K, Miyata H, Yamasaki M, Kurokawa Y, Mori M, Doki Y.
    • Journal Title

      J Surg Oncol.

      Volume: 105 Pages: 189-94

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of Multidetector-row computed tomography to evaluate branch duct type intraductal papillary mucinous neoplasms of the pancreas: influence on surgical decision-making2011

    • Author(s)
      H. Ishii, H. Taniguchi, A. Shiozaki, Y. Kuriu, H. Ikoma, M. Nakanishi, D. Ichikawa, H. Fujiwara, K. Okamoto, T. Ochiai, Y. Kokuba, C. Sakakura, T. Sonoyama, E. Otsuji
    • Journal Title

      Hepatogastroenterology

      Volume: 59 Pages: 115-116

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] REGIV as a potential biomarker for peritoneal dissemination in gastric adenocarcinoma2011

    • Author(s)
      J.H. Moon, Y. Fujiwara, Y. Nakamura, K. Okada, H. Hanada, C. Sakakura, S. Takiguchi, K. Nakajima, H. Miyata, M. Yamasaki, Y. Kurokawa, M. Mori, Y. Doki
    • Journal Title

      J Surg Oncol

      Volume: 10

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic impact of microscopic positive margin in gastric cancer patients2011

    • Author(s)
      T. Nagata, D. Ichikawa, S. Komatsu, K. Inoue, A. Shiozaki, H. Fujiwara, K. Okamoto, C. Sakakura, E. Otsuji
    • Journal Title

      J Surg Oncol

      Volume: 104 Pages: 592-597

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] OSNA-based novel molecular testing for lymph node metastases in colorectal cancer patients: results from a multicenter clinical performance study in Japan2011

    • Author(s)
      H. Yamamoto, M. Sekimoto, M. Oya, N. Yamamoto, F. Konishi, J. Sasaki, S. Yamada, K. Taniyama, H. Tominaga, M. Tsujimoto, H. Akamatsu, A. Yanagisawa, C. Sakakura, Y. Kato, N. Matsuura
    • Journal Title

      Ann Surg Oncol

      Volume: 18 Pages: 1891-1898

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of Cancer Stem Cell Markers CD133 and CD44 in Locoregional Recurrence of Rectal Cancer2011

    • Author(s)
      T. Nagata, C. Sakakura, S. Komiyama, A. Miyashita, M. Nishio, Y. Murayama, S. Komatsu, A. Shiozaki, Y. Kuriu, H. Ikoma, M. Nakanishi, D. Ichikawa, H. Fujiwara, K. Okamoto, T. Ochiai, Y. Kokuba, T. Sonoyama and E. Otsuji
    • Journal Title

      Anticancer Res

      Volume: 31 Pages: 495-500

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] REGIV as a potential biomarker for peritoneal dissemination in gastric adenocarcinoma2011

    • Author(s)
      Moon JH, Fujiwara Y, Nakamura Y, Okada K, Hanada H, Sakakura C, Takiguchi S, Nakajima K, Miyata H, Yamasaki M, Kurokawa Y, Mori M, Doki Y
    • Journal Title

      J Surg Oncol

      Volume: 10

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OSNA-based novel molecular testing for lymph node metastases in colorectal cancer patients : results from a multicenter clinical performance study in Japan2011

    • Author(s)
      Yamamoto H, Sekimoto M, Oya M, Yamamoto N, Konishi F, Sasaki J, Yamada S, Taniyama K, Tominaga H, Tsujimoto M, Akamatsu H, Yanagisawa A, Sakakura C, Kato Y, Matsuura N
    • Journal Title

      Ann Surg Oncol

      Volume: 18 Pages: 1891-1898

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gastrointestinal Stromal Tumor in a Patient with Neurofibromatosis: Abscess Formation in the Tumor Leading to Bacteremia and Seizure2010

    • Author(s)
      M. Kitagawa, T. Koh , N. Nakagawa, Y. Kondo, M. Nishio, A. Oguro, C. Sakakura, M. Nanri, E. Otsuji
    • Journal Title

      Case Rep Gastroenterol

      Volume: 4 Pages: 435-442

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salvage surgery for uncontrollable hepatocellular carcinoma treated with repeated non-surgical therapies2010

    • Author(s)
      T. Ochiai, T. Sonoyama, H. Ikoma, Y. Kuriu, M. Nakanishi, T. Kubota, S. Kikuchi, D. Ichikawa, H. Fujiwara, K. Okamoto, C. Sakakura, Y. Kokuba, E. Otsuji
    • Journal Title

      Hepatogastroenterology

      Volume: 57 Pages: 858-864

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] RUNX3 promoter methylation in colorectal cancer: its relationship with microsatellite instability and its suitability as a novel serum tumor marker2010

    • Author(s)
      M. Nishio, C. Sakakura, T. Nagata, S. Komiyama, A. Miyashita, T. Hamada, Y. Kuryu, H. Ikoma, T. Kubota, A. Kimura, M. Nakanishi, D. Ichikawa, H. Fujiwara, K. Okamoto, T. Ochiai, Y. Kokuba, T. Sonoyama, H. Ida, K. Ito, T. Chiba, Y. Ito, E. Otsuji
    • Journal Title

      Anticancer Res

      Volume: 30 Pages: 2673-2682

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surgical treatment of hepatocellular carcinoma with severe intratumoral arterioportal shunt2010

    • Author(s)
      H. Ishii, T. Sonoyama, S. Nakashima, H. Nagata, A. Shiozaki, Y. Kuriu, H. Ikoma, M. Nakanishi, D. Ichikawa, H. Fujiwara, K. Okamoto, T. Ochiai, Y. Kokuba, C. Sakakura, E. Otsuji
    • Journal Title

      World J Gastroenterol

      Volume: 16 Pages: 3211-3214

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A case report of metastatic neuroendocrine carcinoma of the right adrenal gland successfully treated with chemotherapy and surgery2010

    • Author(s)
      T. Ochiai, S. Komiyama, H. Ikoma, T. Kubota, M. Nakanishi, D. Ichikawa, S. Kikuchi, H. Fujiwara, C. Sakakura,Y. Kokuba, T. Sonoyama, E. Otsuji
    • Journal Title

      Int J Clin Oncol

      Volume: 15 Pages: 423-427

    • NAID

      10026601579

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of polyethylene glycolic acid felt with fibrin sealant to prevent postoperative pancreatic fistula in pancreatic surgery2010

    • Author(s)
      T. Ochiai, T. Sonoyama, K. Soga, K. Inoue, H. Ikoma, A. Shiozaki, Y. Kuriu, T. Kubota, M. Nakanishi, S. Kikuchi, D. Ichikawa, H. Fujiwara, C. Sakakura, K. Okamoto, Y. Kokuba, E. Otsuji
    • Journal Title

      J Gastrointest Surg

      Volume: 14 Pages: 884-890

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue factor pathway inhibitor 2 (TFPI2) is frequently silenced by aberrant promoter hypermethylation in gastric cancer2010

    • Author(s)
      H. Takada, N. Wakabayashi, O. Dohi, K. Yasui, C. Sakakura, S. Mitsufuji, M. Taniwaki, T. Yoshikawa
    • Journal Title

      Cancer Genet Cytogenet

      Volume: 197 Pages: 16-24

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological Characteristics of Colorectal Cancers That Invade the Muscularis Propria2010

    • Author(s)
      A. Miyashita, C. Sakakura, T. Nagata, M. Nishio, T. Hamada, K. Miyagawa, N. Kuriu, H. Ikoma, T. Kubota, M. Nakanishi, D. Ichikawa, S. Kikuchi, H. Fujiwara, K. Okamoto, T. Ochiai, Y. Kokuba, T. Sonoyama, E. Otsuji
    • Journal Title

      J Kyoto Pref Univ Med

      Volume: 119 Pages: 165-174

    • NAID

      40017045841

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic silencing of RELN in gastric cancer2010

    • Author(s)
      O. Dohi, O. Dohi, H. Takada, N. Wakabayashi, K. Yasui, C. Sakakura,S. Mitsufuji, Y. Naito, M. Taniwaki, T. Yoshikawa
    • Journal Title

      Int J Oncol

      Volume: 36 Pages: 85-92

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 新消化管の分子生物学(第10回)胃癌の癌抑制遺伝子RUNX3の分子生物学TGF-β/Wntシグナル伝達系とRUNX3は消化器癌にどのようにかかわっているのか?2010

    • Author(s)
      阪倉長平,大辻英吾
    • Journal Title

      G.I.Research

      Volume: 18 Pages: 151-159

    • Related Report
      2012 Final Research Report
  • [Journal Article] Gastrointestinal Stromal Tumor in a Patient with Neurofibromatosis : Abscess Formation in the Tumor Leading to Bacteremia and Seizure.2010

    • Author(s)
      Kitagawa M, Koh T, Nakagawa N, Kondo Y, Nishio M, Oguro A, Sakakura C, Nanri M, Otsuji E.
    • Journal Title

      Case Rep Gastroenterol.

      Volume: 4(3) Pages: 435-442

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salvage surgery for uncontrollable hepatocellular carcinoma treated with repeated non-surgical therapies2010

    • Author(s)
      Ochiai T, Sonoyama T, Ikoma H, Kuriu Y, Nakanishi M, Kubota T, Kikuchi S, Ichikawa D, Fujiwara H, Okamoto K, Sakakura C, Kokuba Y, Otsuji E.
    • Journal Title

      Hepatogastroenterology.

      Volume: 57(101) Pages: 858-864

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RUNX3 promoter methylation in colorectal cancer : its relationship with microsatellite instability and its suitability as a novel serum tumor marker.2010

    • Author(s)
      Nishio M, Sakakura C, Nagata T, Komiyama S, Miyashita A, Hamada T, Kuryu Y, Ikoma H, Kubota T, Kimura A, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Ochiai T, Kokuba Y, Sonoyama T, Ida H, Ito K, Chiba T, Ito Y, Otsuji E.
    • Journal Title

      Anticancer Res.

      Volume: 30(7) Pages: 2673-2682

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 胃癌の新取扱い規約ガイドラインの治療成績評価の問題点2013

    • Author(s)
      中川登, 今村泰輔, 西村幸寿, 西尾実, 高利守, 阪倉長平, 小黒厚 岡野晋次, 山根哲郎, 安川林良
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 腹腔鏡下部分切除により切除し得た胃glomus腫瘍の一例2013

    • Author(s)
      阪倉長平, 今村泰輔, 西村幸寿, 西尾実, 高利守, 小黒厚, 中川登,
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 胃癌の新取扱い規約ガイドラインの治療成績評価の問題点2012

    • Author(s)
      中川登, 今村泰輔, 西村幸寿, 西尾実, 高利守, 阪倉長平, 小黒厚, 岡野晋次, 山根哲郎, 安川林良
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] 腹腔鏡下部分切除により切除し得た胃glomus 腫瘍の一例2012

    • Author(s)
      阪倉長平, 今村泰輔, 西村幸寿, 西尾実, 高利守, 小黒厚, 中川登
    • Organizer
      第84回日本胃癌学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] stageIV 胃癌の長期生存例 stage 規定因子から見た集学的治療(特に手術適応条件)の検討2012

    • Author(s)
      中川登, 今村泰輔, 西尾実, 高利守, 阪倉長平, 小黒厚, 岡野晋治, 山根哲郎, 安川林良
    • Organizer
      第112回日本外科学会総会
    • Related Report
      2012 Final Research Report
  • [Presentation] 高度肥満症に対する減量消化管手術を安全に行うための手術手技と周術期の要点-われわれの経験から-2012

    • Author(s)
      萩原明於, 小座本雄軌, 戸川剛, 大辻英吾, 阪倉長平, 吉田俊秀, 川村功
    • Organizer
      第112回日本外科学会総会
    • Related Report
      2012 Final Research Report
  • [Presentation] 直腸癌 DST 吻合における縫合不全リスク因子の検討と Covering ileostomy の問題点2012

    • Author(s)
      阪倉長平, 今村泰輔, 西尾実, 高利守, 小黒厚, 中川登
    • Organizer
      第112回日本外科学会総会
    • Related Report
      2012 Final Research Report
  • [Presentation] 肥満症に対する腹腔鏡した襟状胃切除の手術手技・周術期管理の工夫と術後1年以上経過時の治療成績2012

    • Author(s)
      阪倉長平, 小座本雄軌, 戸川剛, 大辻英吾, 萩原明於, 川村 功
    • Organizer
      第67回日本消化器外科学会総会
    • Place of Presentation
      富山
    • Related Report
      2012 Final Research Report
  • [Presentation] 微粒子活性炭による胃リンパ流検索に基づくリンパ節廓清2012

    • Author(s)
      中川登, 今村泰輔, 西村幸寿, 西尾実, 高利守, 阪倉長平, 小黒厚, 岡野晋次, 山根哲郎, 安川林良
    • Organizer
      第67回日本消化器外科学会総会
    • Place of Presentation
      富山
    • Related Report
      2012 Final Research Report
  • [Presentation] 直腸癌術後合併症の検討と対策:DST吻合における縫合不全危険因子の検討とその対策2011

    • Author(s)
      阪倉長平
    • Organizer
      第66回日本消化器外科学会
    • Place of Presentation
      名古屋(シンポジウム)
    • Related Report
      2011 Annual Research Report
  • [Presentation] オンコロジストとしての外科医に求められる基礎研究とは何か 『次世代の標準治療』を探索するための基礎医学研究2010

    • Author(s)
      阪倉長平, 西尾実, 宮下篤史, 長田寛之, 生駒久視, 栗生宣明, 中西正芳, 窪田健, 市川大輔, 藤原斉, 岡本和真, 落合登志哉, 国場幸均, 園山輝久, 大辻英吾
    • Organizer
      第110回日本外科学会総会 パネルディスカッション
    • Place of Presentation
      名古屋
    • Related Report
      2010 Annual Research Report
  • [Remarks] 大学ホームページ

    • URL

      http://www.kpu-m.ac.jp/k/dgstv-surg/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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