Claudin 1 mediates TNFα-induced gene expression and cell migration in human gastric cancer cells.
Project/Area Number |
22591464
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
NAITO Kazuyo 京都府立医科大学, 医学研究科, 教授 (90164102)
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Co-Investigator(Kenkyū-buntansha) |
OTSUJI Eigo 京都府立医科大学, 医学研究科, 教授 (20244600)
SHIOZAKI Atsushi 京都府立医科大学, 医学研究科, 助教 (40568086)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 胃癌 / TNF-α / Claudin-1 / 胃十二指腸外科学 |
Research Abstract |
TNFα has been known to be produced in cancer cells as an endogenous tumor promoter, and involved in epithelial-mesenchymal transition. However, Claudin-1 is well known as one of tight junction proteins, recent studies also showed that the expression of Claudin-1 increased during tumor progression in cancer cells. In this study, we investigated the association between TNFα and Claudin-1 during tumor progression in human gastric cancer cells. Claudin-1 was significantly increased upon TNFα treatment in gastric cancer cells, and down-regulation of Claudin-1 using siRNA inhibited 70% of TNFα-induced gene expression and 87% of microRNA expression. Further, knocking-down of Claudin-1 decreased cell proliferation, migration and invasion, and increased apoptosis. Pathway analysis showed that cellular movement, including MMP7, TGFBR1 and TNFSF10, was the top-ranked signal network. In conclusion, Claudin-1 behaves as a crucial signal mediator in TNFα-induced gene expression and cell migration in gastric cancer cells.
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Report
(4 results)
Research Products
(112 results)
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[Journal Article] Anti-Phosphohistone H3 as an independent prognostic factor in human esophageal squamous cell carcinoma.2013
Author(s)
Nakashima S, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Iitaka D, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Otsuji E.
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Journal Title
Anticancer Res
Volume: 33
Pages: 461-467
Related Report
Peer Reviewed
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[Journal Article] Ki-67 labeling index as an independent prognostic factor in human esophageal squamous cell carcinoma.2012
Author(s)
Sasagawa H, Shiozaki A, Iitaka D, Ichikawa D, Komatsu S, Fujiwara H, Okamoto K, Nakashima S, Kinoshita O, Murayama Y, Kuriu Y, Ikoma H, Nakanishi M, Ochiai T, Kokuba Y, Otsuji E.
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Journal Title
Esophagus
Volume: 9
Issue: 4
Pages: 195-202
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Blockade of Chloride Ion Transport Enhances the Cytocidal Effect of Hypotonic Solution in Gastric Cancer Cells2011
Author(s)
Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, Otsuji E
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Journal Title
J Surg Res
Volume: 176
Issue: 2
Pages: 524-34
DOI
Related Report
Peer Reviewed
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[Journal Article] Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma2011
Author(s)
Kosuga T, Shiozaki A, Ichikawa D, Fujiwara H, Komatsu S, Iitaka D, Tsujiura M, Morimura R, Takeshita H, Nagata H, Okamoto K, Nakahari T, Marunaka Y, Otsuji T
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Journal Title
Oncol Report
Volume: 26
Pages: 577-586
DOI
Related Report
Peer Reviewed
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[Presentation] XB130 regulates expressions of tumor suppressor microRNAs in thyroid cancer cells.2012
Author(s)
Takeshita H, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Murayama Y, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Okamoto K, Fujiwara H, Ochiai T, Kokuba Y, Liu M, Otsuji E
Organizer
日本癌学会学術総会
Place of Presentation
札幌
Related Report
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[Presentation] Roles of xCT, cysteine/glutamate transporter, in esophageal squamous cell carcinoma2012
Author(s)
A. Shiozaki, D. Iitaka, H. Fujiwara, D. Ichikawa, S. Komatsu, K. Okamoto, Y. Murayama, Y. Kuriu, H. Ikoma, M. Nakanishi, T. Ochiai, Y. Kokuba, E. Otsuji
Organizer
13th World Congress of the International Society for Diseases of the Esophagus
Place of Presentation
Venice
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