The development of a novel technique for a rapid diagnosis of nodal metastasis using nanocrystal beads labeled anti-cytokeratine antibody.

• Project/Area Number: 22591471
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kanazawa Medical University
• Principal Investigator: KINAMI Shinichi 金沢医科大学, 医学部, 准教授 (00397184)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: センチネルリンパ節 / 微小転移 / 微小転移診断 / ナノビーズ / 迅速病理診断

Research Abstract

We investigated a novel technique for a rapid diagnosis of nodal metastasis to achieve the prevalence of the sentinel node mapping for early gastric cancer. We tried to visualize metastatic foci in lymph nodes with 3-dimentionnary using the Micro X-CT equipment after the rapid immunohistological whole-mount staining using nanocrystal beads labeled anti-cytokeratine antibody. Regrettably, we failed to obtain any good results because of difficulty in setting of Micro X-CT and finding the low signal/noise regimen of whole-mount immunostaining.