Detection of free intraperitoneal cancer cells and chemosensitivity testing using gene expression analysis for gastric cancer patients

• Project/Area Number: 22591473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: ITO Seiji 愛知県がんセンター(研究所), 腫瘍病理学部, 研究員 (50393129)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 癌 / 微小転移 / 抗癌剤受性 / 抗癌剤感受性 / 胃がん

Research Abstract

This study aimed at integrated development of the genetic detection of peritoneal micrometastases and effective therapy based on biological characteristics of peritoneal micrometastases. Microarray analysis was performed for the cell lines with low CEA expression and new marker genes were identified. The predicting model for peritoneal metastasis was constructed. Both sensitive and resistant cell lines for paclitaxel were establishedandgene expression profiling was compared with parent cell line. The selected genes were validated by clinical samples andidentified as paclitaxel-resistance related genes.

Research Products

• [Journal Article] Establishment and characterization of novel gastric signet-ring cell and non signet-ring cell poorly-differentiated adenocarcinoma cell lines with low and high malignant potential (2013)
  • Author(s): Murakami H, Nakanishi H, Tanaka H, Ito S, Misawa K, Ito Y, Ikehara Y, Kondo E, Kodera Y
  • Journal Title: Gastric Cancer Volume: 16(1) Pages: 74-83
  • Peer Reviewed
• [Journal Article] Long-term follow up of patients who were positive for peritoneal lavage cytology final report from the CCOG0301 study (2012)
  • Author(s): Kodera Y, Ito S, Mochizuki Y, Ohashi N, Tanaka C, Kobayashi D, Kojima H, Matsui T, Kondo K, Fujiwara M.
  • Journal Title: Gastric Cancer Volume: 15 Issue: 3 Pages: 335-337
  • DOI: 10.1007/s10120-012-0156-3
  • NAID: 40019353598
  • Peer Reviewed
• [Journal Article] Establishment and characterization of novel gastric signet-ring cell and non signet-ring cell, poorly-differentiated adenocarcinoma cell lines with low and high malignant potential (2012)
  • Author(s): Murakami H, Nakanishi H, Tanaka H, Ito S, Misawa K, Ito Y, Ikehara Y, Kondo E, Kodera Y.
  • Journal Title: Gastric Cancer Volume: (in press) Issue: 1 Pages: 74-83
  • DOI: 10.1007/s10120-012-0149-2
  • NAID: 40019557355
  • Peer Reviewed
• [Journal Article] The prognostic significance of isolated tumor cells in the lymph nodes of gastric cancer patients (2010)
  • Author(s): Fukagawa T, Sasako M, Ito S, NakanishiH, Iinuma H, Natsugoe S, Katai H, Shimoda T.
  • Journal Title: Gastric Cancer Volume: 13(3) Pages: 191-196
  • Peer Reviewed
• [Journal Article] Phase II Clinical Trial of Postoperative S-1 Monotherapy for Gastric Cancer Patients with Free Intraperitoneal Cancer Cells Detected by Real-Time RT-PCR (2010)
  • Author(s): Ito S, Kodera Y, Mochizuki Y, Kojima T, Nakanishi H, Yamamura Y.
  • Journal Title: World Journal of Surgery Volume: VOL.34(9) Issue: 9 Pages: 2083-2089
  • DOI: 10.1007/s00268-010-0573-6
  • Peer Reviewed
• [Journal Article] The prognostic significance of isolated tumor cells in the lymph nodes of gastric cancer patients. (2010)
  • Author(s): Fukagawa T, Ito S
  • Journal Title: Gastric Cancer Volume: 13 Pages: 191-196
  • Peer Reviewed
• [Presentation] 腹膜播種を伴う胃癌に対する腹腔内投与併用化学療法の有用性を検証する第III相試験(PHOENIXGC試験) (2013)
  • Author(s): 高張大亮、石神浩徳、福島亮治、梨本篤、薮崎裕、小寺泰弘、伊藤誠二、今本治彦、今野元博、藤原義之、田中淳二、上之園芳一、山口拓洋、山口博紀、北山丈二
  • Organizer: 第99回日本消化器病学会総会
  • Place of Presentation: かごしま県民交流センター(鹿児島)
  • Year and Date: 2013-03-23
• [Presentation] 腹膜播種陽性胃癌に対するパクリタキセル腹腔内投与併用化学療法の有用性を検証する第III相試験(PHOENIX-GC試験) (2012)
  • Author(s): 廣野靖夫、石神浩徳、小寺泰弘、福島亮治、今本治彦、今野元博、梨本篤、薮崎裕、上之園芳一、川崎普司、藤原義之、伊藤誠二、山口博紀、北山丈二
  • Organizer: 第74回日本臨床外科学会総会
  • Place of Presentation: 新宿NSビル(東京)
  • Year and Date: 2012-11-29
• [Presentation] Development of rapid isolation device for circulating tumor cells (CTCs) using size-based filtrationmethod and its application for single cell gene expression analysis (2012)
  • Author(s): YusaA,MasudaT, Yamamoto S, Niimi M, Douke H, Okochi M, Toneri M, Ito S, Honda H, Arai F, Nakanishi H.
  • Organizer: 3rd Annual Meeting World CTC
  • Place of Presentation: Hyatt Regency(Boston,USA)
  • Year and Date: 2012-11-13
• [Presentation] 胃がん治療の過去と未来胃がんの集学的治療の近未来 (2012)
  • Author(s): 伊藤誠二、伊藤友一、三澤一成、清水泰博、木下平
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2012-10-25
• [Presentation] ハーセプチン抵抗性を示す新規日本人胃がん由来HER2陽性IHC2+/FISH+胃がん細胞株の樹立とその耐性機構 (2012)
  • Author(s): 中西速夫、近藤千紘、伊藤誠二、伊藤友一、室圭、近藤英作
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館(札幌)
  • Year and Date: 2012-09-21
• [Presentation] HER2陽性胃がん細胞のTrastuzumab耐性株におけるp95HER2およびMUC4の発現 (2012)
  • Author(s): 中西速夫、田中晴成、伊藤誠二、近藤英作
  • Organizer: 第84回日本胃癌学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2012-02-09
• [Presentation] Comprehensive analysis of predictive markers for paclitaxel-resistance against peritoneal metastasis of gastric cancer (2011)
  • Author(s): Murakami H, Ito S, Tanaka H, Ohashi N, Nakayama G, Koike M, Fujiwara M, Kodera Y, Kondo E, Nakanishi H.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] 胃癌腹膜転移に対するPTXの新規感受性予測因子の網羅的探索 (2011)
  • Author(s): 村上弘城
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] Molecular aspect of peritoneal metastasis A new diagnostic and therapeutic strategy in gastric cancer (2011)
  • Author(s): Nakanishi H, Ito S, TsuchidaD, Kodera Y.
  • Organizer: 9th International Gastric Cancer Congress
  • Place of Presentation: Coex Center(Seoul)
  • Year and Date: 2011-04-22
• [Presentation] 胃癌腹膜転移に対するPTX新規感受性予測因子の網羅的探索 (2011)
  • Author(s): 村上弘城
  • Organizer: 第83回日本胃癌学会総会
  • Place of Presentation: 古牧温泉青森屋(青森県)
  • Year and Date: 2011-03-04
• [Presentation] 胃癌腹膜再発予測モデルを用いたmultiple genetic markerの意義の検討 (2010)
  • Author(s): 伊藤誠二
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-04-10
• [Presentation] HER2過剰発現胃癌細胞に対する分子標的治療の検討 (2010)
  • Author(s): 中西速夫、大島由記子、伊藤誠二、三澤一成、小寺泰弘、近藤英作
  • Organizer: 第83回日本胃癌学会総会
  • Place of Presentation: 古牧温泉青森屋(三沢市)
  • Year and Date: 2010-03-05
• [Presentation] ハーセプチン抵抗性を示す新規日本人胃がん由来HER2陽性IHC2+/FISH+胃がん細胞株の樹立とその耐性機構
  • Author(s): 中西速夫
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館（札幌）
• [Presentation] Development of rapid isolation device for circulating tumor cells (CTCs) using size-based filtration method and its application for single cell gene expression analysis
  • Author(s): Yusa A
  • Organizer: ３rd Annual Meeting World CTC
  • Place of Presentation: Hyatt Regency（Boston,USA）
• [Presentation] 腹膜播種陽性胃癌に対するパクリタキセル腹腔内投与併用化学療法の有用性を検証する第III相試験(PHOENIX-GC試験)(
  • Author(s): 廣野靖夫
  • Organizer: 第74回日本臨床外科学会総会
  • Place of Presentation: 新宿NSビル(東京)
• [Presentation] 腹膜播種を伴う胃癌に対する腹腔内投与併用化学療法の有用性を検証する第III相試験(PHOENIX-GC試験)
  • Author(s): 高張大亮
  • Organizer: 第99回日本消化器病学会総会
  • Place of Presentation: かごしま県民交流センター（鹿児島）
• [Presentation] 胃がん治療の過去と未来 胃がんの集学的治療の近未来
  • Author(s): 伊藤 誠二
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Book] "胃癌腹腔内微小転移のメカリズムとその検出"臨床外科 (2013)
  • Author(s): 中西速夫、舎人誠、村上弘城、伊藤友一、三澤一成、伊藤誠二
• [Book] “胃癌腹腔内微小転移のメカリズムとその検出“ 臨床外科 (2013)
  • Author(s): 中西速夫
  • Publisher: 医学書院
• [Book] "In Vivo Imaging" Methods and Protocols, Hoffman, R. M. (ed.), Non invasive and real-time fluorescence imaging ofperitoneal metastasis in nude mice (2012)
  • Author(s): Nakanishi H,Ito S, Matsui M, M isawa K, Kodera Y.
  • Publisher: Humana Press, Totowa, NJ
• [Book] “In Vivo Imaging” Methods and Protocols, Hoffman, R. M. (ed.), Non invasive and real-time fluorescence imaging of peritoneal metastasis in nude mice. (2012)
  • Author(s): Nakanishi H
  • Publisher: Humana Press
• [Book] "In Vivo Imaging" Methods and Protocols (2010)
  • Author(s): Nakanishi, H, Ito S
  • Publisher: Humana Press, Totowa, NJ(in press)
• [Patent(Industrial Property Rights)] 微粒子分離用マイクロ流路チップ、該チップを用いた微粒子分離用システム及び微粒子分離方法 (2012)
  • Inventor(s): 新井史人、益田泰輔、新美京、中西速夫、伊藤誠二
  • Industrial Property Rights Holder: 名古屋大学、愛知県がんセンター
  • Filing Date: 2012-10-15
• [Patent(Industrial Property Rights)] 微粒子分離用マイクロ流路チップ、該チップを用いた微粒子分離用システム (2012)
  • Inventor(s): 新井 史人、益田 泰輔、新美 京、中西 速夫、伊藤 誠二
  • Industrial Property Rights Holder: 名古屋大学、愛知県がんセンター
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-10-15