Elucidation of the anticancer mechanism of the lysophospholid through the nuclear receptor

• Project/Area Number: 22591482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Shinshu University
• Principal Investigator: TSUKAHARA Tamotsu 信州大学, 医学部, 助教 (00529943)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 核内受容体 / リゾリン脂質 / 大腸癌 / 大腸がん / ハイドロゲル

Research Abstract

Cyclic phosphatidic acid (cPA) is a specific, high-affinity antagonist of PPARγ; however, the molecular mechanism by which cPA inhibits colon cancer proliferation remains to be clarified. Our report indicates that PPARγ is expressed at considerable levels in human colon cancer cell lines. cPA suppressed cell growth and induced DNA condensation through PPARγ inhibition. This report suggests that potential use of cPA in the development of drugs targeting colon cancer and possib ly other types of cancer.

Research Products

• [Journal Article] PTB-Associated Splicing Factor (PSF) is a PPAR gamma-Binding Protein and Growth Regulator of Colon Cancer Cells (2013)
  • Author(s): Tamotsu Tsukahara, Hisao Haniu and Yoshikazu Matsuda
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e58749-e58749
  • DOI: 10.1371/journal.pone.0058749
  • Peer Reviewed
• [Journal Article] PPARγ Networks in Cell Signaling: Update and Impact of Cyclic Phosphatidic Acid (2013)
  • Author(s): Tamotsu Tsukahara
  • Journal Title: Journal of Lipids Pages: 1-6
  • URL: http://dx.DOI:.org/10.1155/2013/246597
  • Peer Reviewed
• [Journal Article] Lysophosphatidic Acid Stimulates MCP-1 Secretion from C2C12 Myoblast ISRN (2012)
  • Author(s): Tamotsu Tsukahara and Hisao Haniu
  • Journal Title: Inflammation Volume: 2012 Pages: 1-6
  • DOI: 10.5402/2012/983420
  • Peer Reviewed
• [Journal Article] Murofushi Release of cyclic phosphatidic acid from a gelatin-based hydrogel inhibits colon cancer cell growth and migration (2012)
  • Author(s): Tamotsu Tsukahara and Kimiko Murakami
  • Journal Title: Scientific Reports Volume: 687 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep00687
  • Peer Reviewed
• [Journal Article] Peroxisome Proliferator-Activated Receptor gamma Overexpression Suppresses Proliferation of Human Colon Cancer Cells (2012)
  • Author(s): Tamotsu Tsukahara and Hisao Haniu
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 424 Pages: 524-529
  • URL: http://dx.DOI:.org/10.1016/j.bbrc.2012.06.149
  • Peer Reviewed
• [Journal Article] The Role of PPARγ in the Transcriptional Control by Agonists and Antagonists (2012)
  • Author(s): Tamotsu Tsukahara
  • Journal Title: PPAR research Volume: 2012 Pages: 1-9
  • DOI: 10.1155/2012/362361
  • Peer Reviewed
• [Journal Article] The Role of PPAR_Y in the Transcriptional Control by Agonists and Antagonists (2012)
  • Author(s): Tamotsu Tsukahara
  • Journal Title: PPAR research Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cyclic Phosphatidic Acid Influences the Expression and Regulation of Cyclic Nucleotide Phosphodiesterase 3B and Lipolysis in 3T3-L1 cells (2011)
  • Author(s): Tamotsu Tsukahara, Shuwa Hanazawa, Kimiko Murofushi-Murakami
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 404 Pages: 109-114
  • NAID: 120007110486
  • Peer Reviewed
• [Journal Article] Phospholipase A_1 Activity of Lysophospholipase A-I Links Platelet Activation to LPA Production During Blood Coagulation (2011)
  • Author(s): Alyssa L.Bolen, Anjapravanda P.Naren, Sunitha, Tamotsu Tsukahara, Gabor Tigyi
  • Journal Title: Journal of Lip id Research Volume: 52 Pages: 958-970
  • Peer Reviewed
• [Journal Article] Cyclic Phosphatidic Acid Decreases Proliferation and Survival of Colon Cancer Cells through Inhibition of Peroxisome Proliferator-Activated Receptor γ (2010)
  • Author(s): Tamotsu Tsukahara, Shuwa Hanazawa, Tetsuyuki Kobayashi and Kimiko Murofusi-Murakami
  • Journal Title: Prostaglandins and Other Lipid Mediators Volume: 93 Pages: 126-133
  • URL: http://dx.DOI:.org/10.1016/j.prostaglandins.2010.09.002
  • Peer Reviewed
• [Journal Article] Phospholipase D2-dependent inhibition of the nuclear hormone receptor PPARγ by cyclic phosphatidic acid (2010)
  • Author(s): Tamotsu Tsukahara, Ryoko Tsukahara, Yuko Fujiwara, Juming Yue, Yunhui Cheng, Huazhan Guo, Alyssa Bolen, Chhunxiang Zhang, Louis Balazs, Fabio Re, Guangwei Du, Michael A Frohman, Daniel L. Baker, Abby.L. Parrill, Ayako Uchiyama, Tetsuyuki Kobayashi, Kimiko-Murofushi, and Gabor Tigyi
  • Journal Title: Molecular Cell Volume: 39 Pages: 421-432
  • URL: http://dx.DOI:.org/10.1016/j.molcel.2010.07.022
  • Peer Reviewed
• [Journal Article] Phospholipase D2-dependent inhibition of the nuclear hormone receptor PPAR by cyclic nhosphatidic acid (2010)
  • Author(s): Tamotsu Tsukahara, Ryoko Tsukahara, Yuko Fujiwara, et al.
  • Journal Title: Molecular Cell Volume: 39 Pages: 421-432
  • Peer Reviewed
• [Journal Article] Cyclic phosphatidic acid decreases proliferation and survival of colon cancer cells through inhibition of peroxisome proliferator-activated recentor gamma (2010)
  • Author(s): Tamotsu Tsukahara, Shuwa Ha nazawa, et al.
  • Journal Title: Prostaglandins and Other Lipid Mediators Volume: 93 Pages: 126-133
  • Peer Reviewed
• [Presentation] Release of Cyclic Phosphatidic Acid from a Gelatin-Based Hydrogel Inhibits Colon Cancer Cell Growth and MigrationAnnual Meeting of the Japanese Biochemical Society (2012)
  • Author(s): Tamotsu Tsukahara and Kimiko Murakami-Murofushi
  • Organizer: Japanese Biochemical Society
  • Place of Presentation: Fukuoka, Kyushu, Japan
  • Year and Date: 2012-12-15
• [Presentation] Release of Cyclic Phosphatidic Acid from a Gelatin-Based Hydrogel Inhibits Colon Cancer Cell Growth and Migration (2012)
  • Author(s): Tamotsu Tsukahara
  • Organizer: Annual Meeting of the Japanese Biochemical Society, Japanese Biochemical Society
  • Place of Presentation: 福岡
• [Presentation] Cyclic phosphatidic acid inhibits colon cancer cell proliferation through suppression of peroxisome proliferator-activated receptor gamma (2011)
  • Author(s): Tamotsu Tsukahara and Kimiko Murakami-Murofushi
  • Organizer: ASBMB Annual Meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] Cyclic phosphatidic acid inhibits colon cancer cell proliferation through suppression of peroxisome proliferator-activated receptor gamma (2011)
  • Author(s): Tamotsu Tsukahara, Kimiko Murakami-Murofushi
  • Organizer: ASBMB Annual Meeting 2011
  • Place of Presentation: Washington DC, USA
• [Presentation] Phospholipase D2-dependent Inhibition of the Nuclear Hormone Receptor PPARga mma by Cyclic Phosnhatidic Acid (2010)
  • Author(s): Tamotsu Tsukahara
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Phospholipase D2-dependent inhibition of the nuclear hormone receptor PPARγ by cyclic phosphatidic acid (2010)
  • Author(s): Tamotsu Tsukahara and Gabor Tigyi
  • Organizer: The 83nd Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Kobe, Hyogo, Japan
• [Book] Regulation of the Nuclear Hormone Receptor PPARγ by Endogenous Lysophosphatidic Acids. Lysophospholipid Receptors: Signaling and Biochemistry (edited by Jerold Chun) John Wiley book (ISBN: 978-0-470-56905-4) (2013)
  • Author(s): Ryoko Tsukahara, Tamotsu Tsukahara, Gabor Tigyi
  • Total Pages: 808
• [Book] Regulation of the Nuclear Hormone Receptor PPARγ by Endogenous Lysophosphatidic Acids. Lysophospholipid Receptors: Signaling and Biochemistry (2012)
  • Author(s): Ryoko Tsukahara
  • Publisher: John Wiley book
• [Book] Regulation of the Nuclear Hormone Receptor PPAR_Y by Endogenous Lysophosphatidic Acids. Lysophospholipid Receptors (2012)
  • Author(s): Ryoko Tsukahara, Tamotsu Tsukahahara, Gabor Tigyi
  • Total Pages: 750
  • Publisher: John Wiley Book
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/864
• [Remarks] ホスホリパーゼD2に依存的なPPARγの活性化阻害にはサイクリックフォスファチジ酸が関与する
  • URL: http://first.lifesciencedb.jp/archives/864
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/864
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/864