Regeneration of small intestine with ability of absorption and peristalsis using amniotic membrane and amniotic stem cells

• Project/Area Number: 22591496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Doshisha University
• Principal Investigator: TSUJIMOTO Hiroyuki 同志社大学, 研究開発推進機構, 研究員 (20521272)
• Co-Investigator(Kenkyū-buntansha): HAGIWARA Akeo 同志社大学, 生命医科学部, 教授 (90198648)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小腸大腸肛門外科学 / 再生医学 / 消化管再生 / 羊膜 / 幹細胞

Research Abstract

To overcome the difficulty of regeneration of intestinal epithelial tissues which is the most serious problems for regeneration of fully circular walled intestine with digestion, absorption and peristalsis, we utilized amniotic membrane and amniotic stem cells, which have excellent ability to induce cell and tissue regeneration or pluripotency to differentiate. In the in vitro analysis, the culture on amniotic membrane or co-culture with amniotic stem cells induced the growth and differentiation of the intestinal epithelial cells. However, in the in vivo experiment to reconstruct the intestine using animal models, the intestinal epithelial tissues were not regenerated although the smooth muscle-like tissues were recognized.

Research Products

• [Journal Article] The anti-adhesive effect of thermally cross-linked gelatin film and its influence on the intestinal anastomosis in canine models (2013)
  • Author(s): Hiroyuki Tsujimoto, Ayumi Tanzawa, Mari Matoba, Ayumi Hashimoto, Shuko Suzuki, Shinichiro Morita, Yoshito Ikada, Akeo Hagiwara
  • Journal Title: J Biomed Mater Res Part B Volume: 101B Issue: 1 Pages: 99-109
  • DOI: 10.1002/jbm.b.32821
  • Peer Reviewed
• [Journal Article] 消化器系における神経再生:神経再生を用いる直腸癌の新しい手術戦略 (2012)
  • Author(s): 萩原明於、辻本洋行、阪倉長平、中村達雄
  • Journal Title: GI Research Volume: 20 Pages: 48-55
• [Journal Article] The biological properties of the alkari- treated collagen and gelatin -A preliminary study of their influence on cell growth when used as scaffolds- (2012)
  • Author(s): Chiko Yoshida, Ayumi Tanzawa, Atsushi Tamura, Junki Ikeda, Taichi Orikasa, Hideki Takamori, Shinichiro Morita, Hiroyuki Tsujimoto, Akeo Hagiwara
  • Journal Title: 同志社大学理工学研究報告 Volume: 53 Pages: 7-12
  • Peer Reviewed
• [Journal Article] The biological properties of the alkari-treated collagen and gelatin and gelatin -A preliminary study of their influence on cell growth when used as scaffolds-. (2012)
  • Author(s): Chiko Yoshida, Ayumi Tanzawa, Atsushi Tamura, Junki Ikeda, Taichi Orikasa, Hideki Takamori, Shinichiro Morita, Hiroyuki Tsujimoto, Akeo Hagiwara
  • Journal Title: 同志社大学 理工学研究報告雑誌 Volume: 53(3) Pages: 7-12
  • Peer Reviewed
• [Journal Article] 消化器系における神経再生：神経再生を用いる直腸癌の新しい手術戦略 (2012)
  • Author(s): 萩原明於､辻本洋行､阪倉長平､中村達雄
  • Journal Title: G I Research Volume: 20(6) Pages: 48-55
• [Journal Article] 新規癒着防止材熱架橋ゼラチンフィルムの物理的・生物化学的特性-癒着防止効果の機序及び腸管吻合部への影響に対する基礎的検討 (2011)
  • Author(s): 丹澤あゆみ、辻本洋行、的場麻里、橋本歩、鈴木周子、森田真一郎、筏義人、萩原明郎
  • Journal Title: 同志社大学理工学研究報告 Volume: 52 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Regeneration and functional recovery of intrapelvic nerves removed during extensive surgery by a new artificial nerve conduit: A breakthrough to radical operation for locally advanced and recurrent rectal cancers (2011)
  • Author(s): Hiroyuki Tsujimoto, Tatsuo Nakamura, Tsuneharu Miki, Toshikazu Kubo, Eigo Otsuji, Hisakazu Yamagishi, Akeo Hagiwara
  • Journal Title: J Gastrointest Surg Volume: 15 Pages: 1035-1042
  • Peer Reviewed
• [Journal Article] Endocrine differentiation of rat enterocytes in long-term three- dimensional co-culture with intestinal myofibroblasts (2011)
  • Author(s): Tetsuji Yoshikawa, Shinichi Hamada, Eigo Otsuji, Hiroyuki Tsujimoto, Akeo Hagiwara
  • Journal Title: In Vitro Cell Dev Biol-Animal Volume: 47 Pages: 707-715
  • Peer Reviewed
• [Journal Article] Regeneration and Functional Recovery of Intrapelvic Nerves Removed During Extensive Surgery by a New Artificial Nerve Conduit : A Breakthrough to Radical Operation for Locally Advanced and Recurrent Rectal Cancers (2011)
  • Author(s): Tsujimoto H, Nakamura T, Miki T, Kubo T, Otsuji E, Yamagishi H, Hagiwara A
  • Journal Title: J Gastrointest Surg Volume: 15 Pages: 1035-1042
  • Peer Reviewed
• [Journal Article] Development of a novel ant adhesive material, alginate flakes, ex vivo and in vivo (2011)
  • Author(s): Hirasaki, Y., Fukunaga, M., Kidokoro, A., Hashimoto, A., Nakamura, T., Tsujimoto, H., Hagiwara, A.
  • Journal Title: Surg today Volume: 41 Issue: 7 Pages: 970-977
  • DOI: 10.1007/s00595-010-4410-1
  • Peer Reviewed
• [Journal Article] 新規癒着防止材熱架橋ゼラチンフィルムの物理的・生物学的特性 (2011)
  • Author(s): 丹澤あゆみ、辻本洋行、萩原明郎, 他
  • Journal Title: 同志社大学理工学研究報告 Volume: 52(2) Pages: 150-155
• [Journal Article] Regeneration and Functional Recovery of Intrapelvic Nerves Removed During Extensive Surgery by a New Artificial Nerve Conduit : A Breakthrough to Radical Operation for Locally Advanced and Recurrent Rectal Cancers. (2011)
  • Author(s): Tsujimoto H, Nakamura T, Miki T, Kubo T, Otsuji E, Yamagishi H, Hagiwara A.
  • Journal Title: Journal of Gastrointestinal Surgery
  • Peer Reviewed
• [Journal Article] Development of a novel anti-adhesive material, alginate flakes, ex vivo and in vivo (2011)
  • Author(s): Hirasaki Y, Tsujimoto H, Hagiwara A, et al.
  • Journal Title: Surgery Today Volume: (掲載確定)
  • NAID: 10029048751
  • Peer Reviewed
• [Journal Article] 自動縫合器を用いた肺部分切除におけるポリグリコール酸不織布の空気漏れ防止効果の犬における検討 (2010)
  • Author(s): 橋本歩、平崎憲範、辻本洋行、丹澤あゆみ、的場麻理、桑原正樹、萩原明郎
  • Journal Title: 日本気胸・嚢胞性肺疾患学会誌 Volume: 10(2) Pages: 92-95
  • Peer Reviewed
• [Presentation] 新規癒着防止材熱架橋gelatin filmの生物学的特性 -熱架橋度と癒着防止効果の関係について- (2012)
  • Author(s): 辻本洋行、、丹澤あゆみ、吉田千子、筏義人、萩原明郎
  • Organizer: DDWJ
  • Place of Presentation: 神戸市
  • Year and Date: 2012-10-12
• [Presentation] 再生の足場材料を用いた癒着防止材 -腹膜の再生と消化管吻合部への影響 (2012)
  • Author(s): 辻本洋行、丹澤あゆみ、鈴木周子、筏義人、萩原明郎
  • Organizer: 日本消化器外科学会
  • Place of Presentation: 富山市
  • Year and Date: 2012-07-19
• [Presentation] 再生の足場材料を用いた癒着防止材 -熱架橋gelatin filmによる腹膜の再生と消化管吻合部への影響- (2012)
  • Author(s): 辻本洋行、丹澤あゆみ、吉田千子、筏義人、萩原明郎
  • Organizer: 日本再生医療学会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-13
• [Presentation] 架橋gelatin filmの癒着防止効果と消化管吻合部への影響 -その腹膜再生効果と物理的生物学的特性について- (2012)
  • Author(s): 辻本洋行、丹澤あゆみ、橋本歩、筏義人、萩原明郎
  • Organizer: 日本外科学会
  • Place of Presentation: 千葉市
  • Year and Date: 2012-04-14
• [Presentation] 架橋gelatin filmの癒着防止効果と消化管吻合部への影響 -その腹膜再生効果と物理的・生物学的特性について- (2011)
  • Author(s): 辻本洋行、丹澤あゆみ、橋本歩、筏義人、萩原明郎
  • Organizer: DDWJ
  • Place of Presentation: 博多市
  • Year and Date: 2011-10-23
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と消化管吻合部への影響 (2011)
  • Author(s): 辻本洋行、萩原明郎, 他
  • Organizer: 第9回日本消化器外科学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-10-23
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と腹膜再生 (2011)
  • Author(s): 辻本洋行、萩原明郎, 他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-03-03
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と腹膜再生 (2011)
  • Author(s): 辻本洋行、丹澤あゆみ、橋本歩、筏義人、萩原明郎
  • Organizer: 日本再生医療学会
  • Place of Presentation: 東京都
  • Year and Date: 2011-03-02
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と消化管吻合部への影響 (2011)
  • Author(s): 辻本洋行、丹澤あゆみ、橋本歩、筏義人、萩原明郎
  • Organizer: 日本外科学会
  • Place of Presentation: 誌上開催
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と消化管吻合部への影響 (2011)
  • Author(s): 辻本洋行、萩原明郎, 他
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: (誌上発表)
• [Presentation] 熱架橋gelatin filmを用いた癒着防止と腹膜再生 (2010)
  • Author(s): 辻本洋行、萩原明郎, 他
  • Organizer: 第8回消化器外科学会大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-10-15
• [Presentation] 架橋gelatin filmを用いた癒着防止と腹膜再生 (2010)
  • Author(s): 辻本洋行、丹澤あゆみ、橋本歩、筏義人、萩原明郎
  • Organizer: DDWJ.
  • Place of Presentation: 横浜市
  • Year and Date: 2010-10-14
• [Presentation] 熱架橋gelatin filmの癒着防止効果と消化管吻合部への影響 -その腹膜再生効果と物理的･生物学的特性について-.
  • Author(s): 辻本洋行､丹澤あゆみ､橋本歩､的場麻理､吉田千子､竹本洋一､石橋治昭､鈴木周子､森田真一郎､筏義人､萩原明郎
  • Organizer: 第112回 日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] 再生の足場材料を用いた癒着防止材 –熱架橋gelatin filmによる腹膜の再生と消化管吻合部への影響-.
  • Author(s): 辻本洋行､丹澤あゆみ､折笠太一､池田潤基､吉田千子､鳥井裕子､御座本雄軌､竹本洋一､鈴木周子､平嗣良､森田真一郎､筏義人､萩原明郎
  • Organizer: 第11回 日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 再生の足場材料を用いた癒着防止材 –腹膜の再生と消化管吻合部への影響-.
  • Author(s): 辻本洋行､丹澤あゆみ､折笠太一､池田潤基､御座本雄軌､竹本洋一､鈴木周子､森田真一郎､筏義人､萩原明郎
  • Organizer: 第67回 日本消化器外科学会総会
  • Place of Presentation: 富山国際会議場（富山県）
• [Presentation] 新規癒着防止材熱架橋gelatin filmの生物学的特性 -熱架橋度と癒着防止効果の関係について-.
  • Author(s): 辻本洋行､丹澤あゆみ､吉田千子､堀井常人､宮本博恵､安部里恵､折笠太一､池田潤基､鳥井裕子､御座本雄軌､鈴木周子､森田真一郎､筏義人､萩原明郎
  • Organizer: 第10回 日本消化器外科学会大会
  • Place of Presentation: 神戸国際会議場（兵庫県）