| Project/Area Number |
22591497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
KUNO Takashi 兵庫医科大学, 医学部, 助教 (30441281)
|
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| Co-Investigator(Kenkyū-buntansha) |
MATSUBARA Nagahide 兵庫医科大学, 医学部, 准教授 (70314672)
TAMURA Kazuo 近畿大学, 理工学部, 教授 (20278823)
TOMITA Naoihiro 兵庫医科大学, 医学部, 教授 (00252643)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 大腸癌 / MYH / 発癌 / 修復遺伝子 / colorectal cancer / MUTYH / base excision repair / loss of herozygosity / KRAS |
|---|
| Research Abstract |
The base excision repair gene MUTYH encodes glycosylase which removes adenine residues mispaired with 8-OHG. Biallelic germline mutation of the MUTYH gene are known to cause multiple colorectal adenomas including polyposis and cancer, mostly due to G:C→T:A transversions in proto-ongogenes or tumor suppressor genes. To investigate the possible role of MUTYH mutation in sporadic colorectal cancer (CRC), we examined alterations of the MUTYH gene including somatic mutations and allelic loss in sporadic CRC. MUTYH mutations in cancer DNA were detected in 3 cases, while mutations were also found in DNA samples from the normal tissue, indicating that all were germline mutations. In conclusion, somatic alterations of MUTYH in sporadic CRC were rare, similar to other DNA repair genes.
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