| Project/Area Number |
22591511
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIRANO Satoshi 北海道大学, 大学院・医学研究科, 教授 (50322813)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIKAWA Takahiro 北海道大学, 大学病院, 助教 (50507572)
NAKAMURA Toru 北海道大学, 大学院・医学研究科, 助教 (70645796)
宮本 正樹 北海道大学, 大学病院, 助教 (40333611)
近藤 哲 北海道大学, 大学院・医学研究科, 教授 (30215454)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 胆道癌 / tissue micro array / プロテオミクス / tisssue micro array / 腫瘍マーカー / 生存率 / TMA解析 / マーカー |
|---|
| Research Abstract |
We analyzed resected specimens by the proteomics approach anddetected 4 unique proteins (actinin-1,actinin-4,DJ-1,cathepsin B) out of 38 higher gene expression products compared with normal bile duct tissue. Next,we evaluated theexpression level of these 4 proteins by Western blotting method among 19 bile ductcarcinoma cell lines and characterized them into low expression cell lines and highexpression cell lines. We Finally, we performed immunohistochemical analysisutilizing tissue micro array of the consecutive 96 resected specimens of bile ductcarcinoma Among 4 proteins, high expression of DJ-1 could be a marker to suggestgood prognosis (p=0604)
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