| Project/Area Number |
22591516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MOTOYAMA Hiroaki 信州大学, 医学部附属病院, 助教 (20569587)
MIYAGAWA Shinichi 信州大学, 医学部, 教授 (80229806)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 再生医療 / 遺伝子治療 / 細胞間相互作用 / リプログラミング |
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| Research Abstract |
We introduced Pdx1 and Ngn3 into regenerative liver using non-viral gene transfer technique. Co-expression of Pdx1 and Ngn3 activated the transcription of various islet-related genes, and induced insulin synthesis in the liver. Futhermore, it was suggested that this reprogramming was enhanced by co-expression of triple factors adding v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (MafA).In conclusion, the reprogramming will occur even in vivo without the need for viral vectors. Further investigation might be needed to optimize the combination of transgene.
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