| Project/Area Number |
22591537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAITO Aya 東京大学, 医学部附属病院, 助教 (10431868)
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| Co-Investigator(Kenkyū-buntansha) |
MOTOMURA Noboru 東京大学, 医学部附属病院, 講師 (40332580)
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| Co-Investigator(Renkei-kenkyūsha) |
NOGUCHI Norihisa 東京薬科大学, 薬学部, 準教授
KAKIMI Kazuhiro 東京大学, 医学部附属病院, 特任準教授
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 心臓血管外科学 / MRSA / 心増大血管外科学 |
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| Research Abstract |
Recent experiments with fresh vascular allografts showed suppression of MRSA growth by the induction of immune response and tryptophan metabolism via the IDO pathway. In this study, the impact of cryopreservation on a potential key role in the anti-MRSA resistance was analyzed. Brown Norway and Lewis rats were used for allogeneic transplantation models, and fresh allograft (FA) and cryopreserved allograft (CA) were used as conduits. The grafts were recovered on postoperative day 7 and 14 and submitted for real-time PCR andMRSA proliferation assay. IFNγ, TNFα and IDO gene expression in FA was higher than inCA on days 7 and 14, and both FA and CA groups showed significant suppression of MRSA growth compare to control group. In conclusion, both FA and CA suppressed MRSA proliferation. IDO expression in CA was lower than in FA, but sufficient to suppress MRSA growth was expected.
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