Construction of 3D cardiac tissue: alignment and maturation of cells, and improvement of contraction force by extensional stimulation

• Project/Area Number: 22591552
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nara Medical University
• Principal Investigator: TANIGUCHI Shigeki 奈良県立医科大学, 胸部・心臓血管外科, 教授 (90183467)
• Co-Investigator(Kenkyū-buntansha): NAITO Hiroshi 公立大学法人 奈良県立医科大学, 胸部・心臓血管外科, 助教 (00316069)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医療 / 組織工学 / 細胞・組織 / 心筋細胞 / ES細胞 / 人工心筋組織

Research Abstract

Culture condition for constructing artificial cardiac tissue was examined. Housemade collagen increased the ratio of cardiac cells in the tissue, the expression of genes relating calcium handling, and the ratio of protein for contraction compared with commercial available collagen. It was also revealed that three-dimensional culture in collagen hydrogel itself might induce differentiation of ES cells into cardiac cells.