Detection of new fusion partner genes with ALK gene

• Project/Area Number: 22591563
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: SONOBE Makoto 京都大学, 医学研究科, 講師 (00432378)
• Co-Investigator(Kenkyū-buntansha): DATE Herushi 京都大学, 医学(系)研究科(研究院), 教授 (60252962) ; TAKAHASHI Tiyoshi (90527079) ; MENJYU Toshi 京都大学, 医学研究科, 助教 (30527081) ; 宮原 亮 京都大学, 医学研究科, 講師 (20335285)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 原発性肺癌 / 腺癌 / ALK / 融合遺伝子

Research Abstract

We analyzed fusion gene(s) between ALK gene and EML4 or other unknown genes using surgically resectedlung cancer specimens. And easy and effective screening method for detecting these fusion genes related to ALK gene. We have established a fluorescent immunohistochemistry (FISH) method detecting ALK fusion genes easily to place a red probe in the head of ALK gene and a green probe in the exon 20 of ALK gene, which become luminescent apart each other when the ALK fusion gene exists. Because of low incidence of ALK fusion gene in lung cancer population (under 5% of resected cases), we have established an algorithm for ALK fusion gene detection. In the algorithm, tumors those are acinar predominant adenocarcinoma, without epidermal growth factor receptor gene mutations, and of patients who are never or light smokers (20 pack-year or lower) are selected for candidates of ALK fusion gene detection. To apply the algorithm, pretest probability of positive ALK fusion genes increases up to approximately 25%.

Research Products

• [Journal Article] Detection of ALK fusion in lung cancer using fluorescence in situ hybridization (2012)
  • Author(s): Kobayashi M, Sonobe M, Takahashi T, Yoshizawa A, Kikuchi R, Date H
  • Journal Title: Asian Cardiovasc Thorac Ann Volume: 20 Pages: 426-31
• [Journal Article] Detection of ALK fusion in lung cancer using fluorescence in situ hybridization. (2012)
  • Author(s): Kobayashi M, Sonobe M, Takahashi T, Yoshizawa A, Kikuchi R, Date H.
  • Journal Title: Asian Cardiovasc Thorac Ann. Volume: 20 Pages: 426-31
  • Peer Reviewed
• [Journal Article] Clinicopathologic features of non-small cell lung cancer with EML4-ALK fusion gene (2010)
  • Author(s): Takahashi T, Sonobe M, Kobayashi M, Yoshizawa A, Menju T, Nakayama E, Mino N, Iwakiri S, Sato K, Miyahara R, Okubo K, Manabe T, Date H
  • Journal Title: Ann Surg Oncol Volume: 17 Pages: 889-897
  • NAID: 120002036596
• [Journal Article] Clinicopathologic features of non-small cell lung cancer with EML4-ALK gene. (2010)
  • Author(s): Takahashi T, Sonobe M, et al.
  • Journal Title: Ann Surg Oncol Volume: 17 Pages: 889-897
  • Peer Reviewed
• [Presentation] 非小細胞肺癌における EML4-ALK 融合遺伝子の臨床病理学的検討 (2010)
  • Author(s): 小林正嗣、園部誠、石川将史、菊地柳太郎、喜多村次郎、中山英、毛受暁史、高橋剛士、大久保憲一、伊達洋至
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-13
• [Presentation] 術後73ヶ月後に再発したEML4-ALK融合遺伝子を有する原発性肺癌の一例 (2010)
  • Author(s): 高橋剛士、園部誠, 他
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台市、宮城県
  • Year and Date: 2010-05-13
• [Presentation] 非小細胞肺癌におけるEML4-ALK融合遺伝子の臨床病理学的検討 (2010)
  • Author(s): 小林正嗣、園部誠, 他
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台市、宮城県
  • Year and Date: 2010-05-13
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/