Investigation about the mechanism that lung cancer growth is accelerated in emphysematous lungs

• Project/Area Number: 22591568
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Yamaguchi University
• Principal Investigator: HAYASHI Masataro 山口大学, 医学部附属病院, 助教 (00554057)
• Co-Investigator(Kenkyū-buntansha): UEDA Kazuhiro 山口大学, 医学部附属病院, 講師 (90420520) ; 田中 俊樹 山口大学, 医学部附属病院, 助教 (50457305)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 肺気腫 / 肺癌 / 癌細胞増殖 / 癌血管新生 / MMP-2 / MT1-MMP / 肺腫瘍 / MMP / MMP2 / 免疫染色

Research Abstract

A mouse model of pulmonary emphysema was induced by intratracheal nebulization of elastase. Intravenous administration of Lewis lung carcinoma (3LL) cells in the mice resulted in three-fold increase in metastatic foci with a diameter of no less than 2mm on the surface of the lungs. According to an in vitro study, proliferation of 3LL cells was promoted by incubation with the conditioned medium containing pneumocytes of the model mice for 48 hours. The latent form of MMP-2 was overexpressed in monocultures of the pneumocytes from the model mice. The active form of MMP-2 and MT1-MMP were synchronously increased in the conditioned medium with the incubation of 3LL cells. The PCNA proliferation index and MVD-CD105+was higher in cancer tissue from patients with emphysema than in those from patients without. The MMP-2 expression intensity was also stronger in cancer cells and cancer stromal cells from patients with emphysema than in those from patients without.

Research Products

• [Presentation] 肺気腫は肺腫瘍増大に好適な微小環境である:MT1-MMP/MMP-2 axis の関与 (2013)
  • Author(s): 村上順一、林雅太郎、上田和弘、李桃生、濱野公一
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場(福岡)
• [Presentation] Pulmonary emphysema as a tumor-friendly microenvironment: Role of the MT1-MMP/MMP-2 axis (2013)
  • Author(s): Junichi Murakami, Kazuhiro Ueda, Arata Nishimoto, Masataro Hayashi, and Kimikazu Hamano
  • Organizer: American Association for Cancer research (AACR) ANNUAL MEETING 2013
  • Place of Presentation: WashingtonD.C.(USA)
• [Presentation] Pulmonary emphysema as a tumor-friendly microenvironment: Role of the MT1-MMP/MMP-2 axis (2013)
  • Author(s): Junichi Murakami
  • Organizer: AACR Annual Meeting 2013
  • Place of Presentation: Washington (USA)
• [Presentation] 肺気腫は肺腫瘍増大に好適な微小環境である:MT1-MMP/MMP-2 axis の関与 (2012)
  • Author(s): 村上順一、林雅太郎、上田和弘、濱野公一
  • Organizer: 第65回日本胸部外科学会定期学術大会
  • Place of Presentation: 福岡国際会議場(福岡)
• [Presentation] Tumor growth is accelerated in emphysematous lungs via the MT1-MMP/MMP-2 axis (2012)
  • Author(s): 村上順一、西本新、上田和弘
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌)
• [Presentation] Tumor growth is accelerated in emphysematous lungs via the MT1-MMP/MMP-2 axis（肺気腫ではMT1-MMP/MMP-2 axisを介して肺腫瘍の進展が促進する） (2012)
  • Author(s): 村上順一
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（札幌）
• [Remarks] ホームページ:山口大学大学院器官病態外科
  • URL: http://ds.cc.yamaguchi-u.ac.jp/~surg-1/