Antitumor effect of Chelated Endostatin

• Project/Area Number: 22591572
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nagoya City University
• Principal Investigator: YANO Motoki 名古屋市立大学, 大学院・医学研究科, 准教授 (40315883)
• Co-Investigator(Kenkyū-buntansha): SASAKI Hidefumi 名古屋市立大学, 大学院・医学研究科, 准教授 (00336695) ; YOKOTA Keisuke 名古屋市立大学, 大学院・医学研究科, 臨床研究医 (30569257)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エンドスタチン / 呼吸器外科学

Research Abstract

We have assessed antitumor effect of chelated endostatin generating vector which secret chelated endostatin. We injected Lewis lung cancer cells (1×10^6) at the lateral abdominal wall of the BALBC mice. When the produced cancer nodule became 100mm^3in a volume, we started chemotherapy using endostatin transfection and/or anticancer agents (DTX, 5-FU). Then antitumor effect was assessed. Endostatin tranfection was performed by direct injection with plasmids surrounding the cancer nodule. Unfortunately we could not find any advantages of endostatin transfection accumulating anticancer agents.